Development, Vol 121, Issue 6 1875-1884, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

Suppressor of Hairless is required for signal reception during lateral inhibition in the Drosophila pupal notum

F Schweisguth
Institut Jacques Monod, CNRS-Universite Paris VII, France.

Suppressor of Hairless (Su(H)) activity is zygotically required in larval imaginal discs for the singling out of adult sense organ precursor (SOP) cells: loss of Su(H) function results in too many proneural cluster cells adopting the SOP fate, while overexpression of the Su(H) protein prevents SOP specification. Su(H) null mutant alleles are recessive lethal at the late larval and early pupal stages. The development of Su(H) mutant cells in pupae was therefore studied in somatic clones. Clonal analysis first showed that Su(H) is required for the regular spacing of microchaete precursor cells, as clusters of mutant SOPs were detected at positions where singled out sense organ cells are normally found. Second, Su(H) mutant SOPs produced neuron-like cells, consistent with a late defect in Notch (N) signalling. Third, a careful cell-by-cell analysis of clone borders showed that Su(H) mutant cells may adopt the SOP fate even when directly adjacent to wild-type cells. Finally, quantitative clone border analysis indicates that the relative level of Su(H) gene dosage appears to bias the selection of the future SOP: cells with a higher level of Su(H) activity are more likely to adopt the epidermal fate. These results show that notum cells strictly require Su(H) activity for receiving the lateral inhibitory signal. Thus, the DNA-binding protein encoded by the Su(H) gene may act downstream of the N receptor to implement the epidermal, non-SOP fate.

This article has been cited by other articles:

				B. Castro, S. Barolo, A. M. Bailey, and J. W. Posakony Lateral inhibition in proneural clusters: cis-regulatory logic and default repression by Suppressor of Hairless Development, August 1, 2005; 132(15): 3333 - 3344. [Abstract] [Full Text] [PDF]

				C. Sanchez-Irizarry, A. C. Carpenter, A. P. Weng, W. S. Pear, J. C. Aster, and S. C. Blacklow Notch Subunit Heterodimerization and Prevention of Ligand-Independent Proteolytic Activation Depend, Respectively, on a Novel Domain and the LNR Repeats Mol. Cell. Biol., November 1, 2004; 24(21): 9265 - 9273. [Abstract] [Full Text] [PDF]

				S. Koelzer and T. Klein A Notch-independent function of Suppressor of Hairless during the development of the bristle sensory organ precursor cell of Drosophila Development, May 1, 2003; 130(9): 1973 - 1988. [Abstract] [Full Text] [PDF]

				B Kerber, I Monge, M Mueller, P. Mitchell, and S. Cohen The AP-2 transcription factor is required for joint formation and cell survival in Drosophila leg development Development, January 4, 2001; 128(8): 1231 - 1238. [Abstract] [PDF]

				F. Chanut, A. Luk, and U. Heberlein A Screen for Dominant Modifiers of roDom, a Mutation That Disrupts Morphogenetic Furrow Progression in Drosophila, Identifies Groucho and Hairless as Regulators of atonal Expression Genetics, November 1, 2000; 156(3): 1203 - 1217. [Abstract] [Full Text]

				C. S. Wesley and L. Saez Analysis of Notch Lacking the Carboxyl Terminus Identified in Drosophila Embryos J. Cell Biol., May 1, 2000; 149(3): 683 - 696. [Abstract] [Full Text] [PDF]

				F. Schweisguth Dominant-negative mutation in the beta 2 and beta 6 proteasome subunit genes affect alternative cell fate decisions in the Drosophila sense organ lineage PNAS, September 28, 1999; 96(20): 11382 - 11386. [Abstract] [Full Text] [PDF]

				N. Hawkins and G. Garriga Asymmetric cell division: from A to Z Genes & Dev., December 1, 1998; 12(23): 3625 - 3638. [Full Text]

				I. Greenwald LIN-12/Notch signaling: lessons from worms and flies Genes & Dev., June 15, 1998; 12(12): 1751 - 1762. [Full Text]

				P Ligoxygakis, S. Yu, C Delidakis, and N. Baker A subset of notch functions during Drosophila eye development require Su(H) and the E(spl) gene complex Development, January 8, 1998; 125(15): 2893 - 2900. [Abstract] [PDF]

				S Sotillos, F Roch, and S Campuzano The metalloprotease-disintegrin Kuzbanian participates in Notch activation during growth and patterning of Drosophila imaginal discs Development, January 12, 1997; 124(23): 4769 - 4779. [Abstract] [PDF]

				S Wang, S Younger-Shepherd, L. Jan, and Y. Jan Only a subset of the binary cell fate decisions mediated by Numb/Notch signaling in Drosophila sensory organ lineage requires Suppressor of Hairless Development, January 11, 1997; 124(22): 4435 - 4446. [Abstract] [PDF]

				M. Leviten, E. Lai, and J. Posakony The Drosophila gene Bearded encodes a novel small protein and shares 3' UTR sequence motifs with multiple Enhancer of split complex genes Development, January 10, 1997; 124(20): 4039 - 4051. [Abstract] [PDF]

				C. Neumann and S. Cohen A hierarchy of cross-regulation involving Notch, wingless, vestigial and cut organizes the dorsal/ventral axis of the Drosophila wing Development, January 11, 1996; 122(11): 3477 - 3485. [Abstract] [PDF]

				J. de Celis, J de Celis, P Ligoxygakis, A Preiss, C Delidakis, and S Bray Functional relationships between Notch, Su(H) and the bHLH genes of the E(spl) complex: the E(spl) genes mediate only a subset of Notch activities during imaginal development Development, January 9, 1996; 122(9): 2719 - 2728. [Abstract] [PDF]

				M Gho, M Lecourtois, G Geraud, J. Posakony, and F Schweisguth Subcellular localization of Suppressor of Hairless in Drosophila sense organ cells during Notch signalling Development, January 6, 1996; 122(6): 1673 - 1682. [Abstract] [PDF]

				P Heitzler, M Bourouis, L Ruel, C Carteret, and P Simpson Genes of the Enhancer of split and achaete-scute complexes are required for a regulatory loop between Notch and Delta during lateral signalling in Drosophila Development, January 1, 1996; 122(1): 161 - 171. [Abstract] [PDF]

				M Lecourtois and F Schweisguth The neurogenic suppressor of hairless DNA-binding protein mediates the transcriptional activation of the enhancer of split complex genes triggered by Notch signaling. Genes & Dev., November 1, 1995; 9(21): 2598 - 2608. [Abstract] [PDF]

				A M Bailey and J W Posakony Suppressor of hairless directly activates transcription of enhancer of split complex genes in response to Notch receptor activity. Genes & Dev., November 1, 1995; 9(21): 2609 - 2622. [Abstract] [PDF]

				F. Diaz-Benjumea and S. Cohen Serrate signals through Notch to establish a Wingless-dependent organizer at the dorsal/ventral compartment boundary of the Drosophila wing Development, January 12, 1995; 121(12): 4215 - 4225. [Abstract] [PDF]