|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 121, Issue 9 2937-2947, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
D McKearin and B Ohlstein
Department of Biochemistry, University of Texas-Southwestern Medical Center, Dallas 75235-9038, USA.
Cell differentiation commonly dictates a change in the cell cycle of mitotic daughters. Previous investigations have suggested that the Drosophila bag of marbles (bam) gene is required for the differentiation of germline stem cell daughters (cystoblasts) from the mother stem cells, perhaps by altering the cell cycle. In this paper, we report the preparation of antibodies to the Bam protein and the use of those reagents to investigate how Bam is required for germ cell development. We find that Bam exists as both a fusome component and as cytoplasmic protein and that cytoplasmic and fusome Bam might have separable activities. We also show that bam mutant germ cells are blocked in differentiation and are trapped as mitotically active cells like stem cells. A model for how Bam might regulate cystocyte differentiation is presented.
This article has been cited by other articles:
|
|
 |
|
  T. Xie, X. Song, Z. Jin, L. Pan, C. Weng, S. Chen, and N. Zhang Interactions between Stem Cells and Their Niche in the Drosophila Ovary Cold Spring Harb Symp Quant Biol, November 6, 2008; (2008) sqb.2008.73.014v1. [Abstract] [PDF]
|
|
|
|
|
|
A.C. Spradling, T. Nystul, D. Lighthouse, L. Morris, D. Fox, R. Cox, T. Tootle, R. Frederick, and A. Skora Stem Cells and Their Niches: Integrated Units That Maintain Drosophila Tissues Cold Spring Harb Symp Quant Biol, November 6, 2008; (2008) sqb.2008.73.023v1. [Abstract] [PDF]
|
|
|
|
 |
|
 D. A. Dansereau and P. Lasko RanBPM regulates cell shape, arrangement, and capacity of the female germline stem cell niche in Drosophila melanogaster J. Cell Biol., September 8, 2008; 182(5): 963 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Flatt, K.-J. Min, C. D'Alterio, E. Villa-Cuesta, J. Cumbers, R. Lehmann, D. L. Jones, and M. Tatar Drosophila germ-line modulation of insulin signaling and lifespan PNAS, April 29, 2008; 105(17): 6368 - 6373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Narbonne-Reveau, S. Senger, M. Pal, A. Herr, H. E. Richardson, M. Asano, P. Deak, and M. A. Lilly APC/CFzr/Cdh1 promotes cell cycle progression during the Drosophila endocycle Development, April 15, 2008; 135(8): 1451 - 1461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, Z. Li, and Y. Cai The JAK/STAT pathway positively regulates DPP signaling in the Drosophila germline stem cell niche J. Cell Biol., February 25, 2008; 180(4): 721 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yang, D. Chen, R. Duan, L. Xia, J. Wang, A. Qurashi, P. Jin, and D. Chen Argonaute 1 regulates the fate of germline stem cells in Drosophila Development, December 1, 2007; 134(23): 4265 - 4272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Bogard, L. Lan, J. Xu, and R. S. Cohen Rab11 maintains connections between germline stem cells and niche cells in the Drosophila ovary Development, October 1, 2007; 134(19): 3413 - 3418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Z. Maines, J. K. Park, M. Williams, and D. M. McKearin Stonewalling Drosophila stem cell differentiation by epigenetic controls Development, April 15, 2007; 134(8): 1471 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Roper Rtnl1 is enriched in a specialized germline ER that associates with ribonucleoprotein granule components J. Cell Sci., March 15, 2007; 120(6): 1081 - 1092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Niki, T. Yamaguchi, and A. P. Mahowald Establishment of stable cell lines of Drosophila germ-line stem cells PNAS, October 31, 2006; 103(44): 16325 - 16330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Casper and M. Van Doren The control of sexual identity in the Drosophila germline Development, August 1, 2006; 133(15): 2783 - 2791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Siddall, E. A. McLaughlin, N. L. Marriner, and G. R. Hime The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity PNAS, May 30, 2006; 103(22): 8402 - 8407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Civetta, S. A. Rajakumar, B. Brouwers, and J. P. Bacik Rapid Evolution and Gene-Specific Patterns of Selection for Three Genes of Spermatogenesis in Drosophila Mol. Biol. Evol., March 1, 2006; 23(3): 655 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Xi, C. Doan, D. Liu, and T. Xie Pelota controls self-renewal of germline stem cells by repressing a Bam-independent differentiation pathway Development, December 15, 2005; 132(24): 5365 - 5374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Jin, E. M. Homola, P. Goldbach, Y. Choi, J. A. Brill, and S. D. Campbell Drosophila Myt1 is a Cdk1 inhibitory kinase that regulates multiple aspects of cell cycle behavior during gametogenesis Development, September 15, 2005; 132(18): 4075 - 4085. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Jiang, X. Ye, X. Liu, L. Fincher, D. McKearin, and Q. Liu Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila Genes & Dev., July 15, 2005; 19(14): 1674 - 1679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Z. Morris, A. Hong, M. A. Lilly, and R. Lehmann twin, a CCR4 homolog, regulates cyclin poly(A) tail length to permit Drosophila oogenesis Development, March 15, 2005; 132(6): 1165 - 1174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gilboa and R. Lehmann How different is Venus from Mars? The genetics of germ-line stem cells in Drosophila females and males Development, October 15, 2004; 131(20): 4895 - 4905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. Snapp, T. Iida, D. Frescas, J. Lippincott-Schwartz, and M. A. Lilly The Fusome Mediates Intercellular Endoplasmic Reticulum Connectivity in Drosophila Ovarian Cysts Mol. Biol. Cell, October 1, 2004; 15(10): 4512 - 4521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Schulz, A. A. Kiger, S. I. Tazuke, Y. M. Yamashita, L. C. Pantalena-Filho, D. L. Jones, C. G. Wood, and M. T. Fuller A Misexpression Screen Reveals Effects of bag-of-marbles and TGF{beta} Class Signaling on the Drosophila Male Germ-Line Stem Cell Lineage Genetics, June 1, 2004; 167(2): 707 - 723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. O. Casanueva and E. L. Ferguson Germline stem cell number in the Drosophila ovary is regulated by redundant mechanisms that control Dpp signaling Development, May 1, 2004; 131(9): 1881 - 1890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Song, M. D. Wong, E. Kawase, R. Xi, B. C. Ding, J. J. McCarthy, and T. Xie Bmp signals from niche cells directly repress transcription of a differentiation-promoting gene, bag of marbles, in germline stem cells in the Drosophila ovary Development, March 15, 2004; 131(6): 1353 - 1364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gilboa, A. Forbes, S. I. Tazuke, M. T. Fuller, and R. Lehmann Germ line stem cell differentiation in Drosophila requires gap junctions and proceeds via an intermediate state Development, December 29, 2003; 130(26): 6625 - 6634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Ohlmeyer and T. Schupbach Encore facilitates SCF-Ubiquitin-proteasome-dependent proteolysis during Drosophila oogenesis Development, December 22, 2003; 130(25): 6339 - 6349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. K. Smulders-Srinivasan and H. Lin Screens for piwi Suppressors in Drosophila Identify Dosage-Dependent Regulators of Germline Stem Cell Division Genetics, December 1, 2003; 165(4): 1971 - 1991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Niki and A. P. Mahowald Ovarian cystocytes can repopulate the embryonic germ line and produce functional gametes PNAS, November 25, 2003; 100(24): 14042 - 14045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Mulligan Robert C. King: An Appreciation of His Work Genetics, November 1, 2003; 165(3): 939 - 943. [Full Text] [PDF]
|
|
|
|
|
|
J. Z. Morris, C. Navarro, and R. Lehmann Identification and Analysis of Mutations in bob, Doa and Eight New Genes Required for Oocyte Specification and Development in Drosophila melanogaster Genetics, August 1, 2003; 164(4): 1435 - 1446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Zhu and T. Xie Clonal expansion of ovarian germline stem cells during niche formation in Drosophila Development, June 15, 2003; 130(12): 2579 - 2588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Chen and D. M. McKearin A discrete transcriptional silencer in the bam gene determines asymmetric division of the Drosophila germline stem cell Development, March 15, 2003; 130(6): 1159 - 1170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mathe, Y. H. Inoue, W. Palframan, G. Brown, and D. M. Glover Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis Development, March 1, 2003; 130(5): 901 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Besse and A.-M. Pret Apoptosis-mediated cell death within the ovarian polar cell lineage of Drosophila melanogaster Development, March 1, 2003; 130(5): 1017 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Besse, D. Busson, and A.-M. Pret Fused-dependent Hedgehog signal transduction is required for somatic cell differentiation during Drosophila egg chamber formation Development, September 1, 2002; 129(17): 4111 - 4124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Tulina and E. Matunis Control of Stem Cell Self-Renewal in Drosophila Spermatogenesis by JAK-STAT Signaling Science, December 21, 2001; 294(5551): 2546 - 2549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Munn and R. Steward The shut-down Gene of Drosophila melanogaster Encodes a Novel FK506-Binding Protein Essential for the Formation of Germline Cysts During Oogenesis Genetics, September 1, 2000; 156(1): 245 - 256. [Abstract] [Full Text]
|
|
|
|
|
|
S. L. Page, K. S. McKim, B. Deneen, T. L. Van Hook, and R. S. Hawley Genetic Studies of mei-P26 Reveal a Link Between the Processes That Control Germ Cell Proliferation in Both Sexes and Those That Control Meiotic Exchange in Drosophila Genetics, August 1, 2000; 155(4): 1757 - 1772. [Abstract] [Full Text]
|
|
|
|
|
|
B. Ohlstein, C. A. Lavoie, O. Vef, E. Gateff, and D. M. McKearin The Drosophila Cystoblast Differentiation Factor, benign gonial cell neoplasm, Is Related to DExH-box Proteins and Interacts Genetically With bag-of-marbles Genetics, August 1, 2000; 155(4): 1809 - 1819. [Abstract] [Full Text]
|
|
|
|
|
|
J Huynh and D St Johnston The role of BicD, egl, orb and the microtubules in the restriction of meiosis to the Drosophila oocyte Development, January 7, 2000; 127(13): 2785 - 2794. [Abstract] [PDF]
|
|
|
|
|
|
C Pare and B Suter Subcellular localization of Bic-D::GFP is linked to an asymmetric oocyte nucleus J. Cell Sci., January 6, 2000; 113(12): 2119 - 2127. [Abstract] [PDF]
|
|
|
|
|
|
D. Cox, A Chao, and H Lin piwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells Development, January 2, 2000; 127(3): 503 - 514. [Abstract] [PDF]
|
|
|
|
|
|
A. León and D. McKearin Identification of TER94, an AAA ATPase Protein, as a Bam-dependent Component of the Drosophila Fusome Mol. Biol. Cell, November 1, 1999; 10(11): 3825 - 3834. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Kim-Ha, J. Kim, and Y.-J. Kim Requirement of RBP9, a Drosophila Hu Homolog, for Regulation of Cystocyte Differentiation and Oocyte Determination during Oogenesis Mol. Cell. Biol., April 1, 1999; 19(4): 2505 - 2514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z Liu, T Xie, and R Steward Lis1, the Drosophila homolog of a human lissencephaly disease gene, is required for germline cell division and oocyte differentiation Development, January 10, 1999; 126(20): 4477 - 4488. [Abstract] [PDF]
|
|
|
|
|
|
M. Giansanti, S Bonaccorsi, and M Gatti The role of anillin in meiotic cytokinesis of Drosophila males J. Cell Sci., January 7, 1999; 112(14): 2323 - 2334. [Abstract] [PDF]
|
|
|
|
|
|
F. King and H Lin Somatic signaling mediated by fs(1)Yb is essential for germline stem cell maintenance during Drosophila oogenesis Development, January 5, 1999; 126(9): 1833 - 1844. [Abstract] [PDF]
|
|
|
|
|
|
D. N. Cox, A. Chao, J. Baker, L. Chang, D. Qiao, and H. Lin A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal Genes & Dev., December 1, 1998; 12(23): 3715 - 3727. [Abstract] [Full Text]
|
|
|
|
|
|
M. Pepling and A. Spradling Female mouse germ cells form synchronously dividing cysts Development, January 9, 1998; 125(17): 3323 - 3328. [Abstract] [PDF]
|
|
|
|
|
|
M de Cuevas and A. Spradling Morphogenesis of the Drosophila fusome and its implications for oocyte specification Development, January 8, 1998; 125(15): 2781 - 2789. [Abstract] [PDF]
|
|
|
|
|
|
L. Kadyk and J Kimble Genetic regulation of entry into meiosis in Caenorhabditis elegans Development, January 5, 1998; 125(10): 1803 - 1813. [Abstract] [PDF]
|
|
|
|
|
|
A Gonzalez-Reyes, H Elliott, and D St Johnston Oocyte determination and the origin of polarity in Drosophila: the role of the spindle genes Development, January 12, 1997; 124(24): 4927 - 4937. [Abstract] [PDF]
|
|
|
|
|
|
P Gonczy, E Matunis, and S DiNardo bag-of-marbles and benign gonial cell neoplasm act in the germline to restrict proliferation during Drosophila spermatogenesis Development, January 11, 1997; 124(21): 4361 - 4371. [Abstract] [PDF]
|
|
|
|
|
|
E Matunis, J Tran, P Gonczy, K Caldwell, and S DiNardo punt and schnurri regulate a somatically derived signal that restricts proliferation of committed progenitors in the germline Development, January 11, 1997; 124(21): 4383 - 4391. [Abstract] [PDF]
|
|
|
|
|
|
B Ohlstein and D McKearin Ectopic expression of the Drosophila Bam protein eliminates oogenic germline stem cells Development, January 9, 1997; 124(18): 3651 - 3662. [Abstract] [PDF]
|
|
|
|
|
|
H Lin and A. Spradling A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary Development, January 6, 1997; 124(12): 2463 - 2476. [Abstract] [PDF]
|
|
|
|
|
|
C. Lehner and M. Lane Cell cycle regulators in Drosophila: downstream and part of developmental decisions J. Cell Sci., January 3, 1997; 110(5): 523 - 528. [Abstract] [PDF]
|
|
|
|
|
|
A.C. Spradling, M. de Cuevas, D. Drummond-Barbosa, L. Keyes, M. Lilly, M. Pepling, and T. Xie The Drosophila Germarium: Stem Cells, Germ Line Cysts, and Oocytes Cold Spring Harb Symp Quant Biol, January 1, 1997; 62(0): 25 - 34. [Abstract] [PDF]
|
|
|
|
|
|
G. Hime, J. Brill, and M. Fuller Assembly of ring canals in the male germ line from structural components of the contractile ring J. Cell Sci., January 12, 1996; 109(12): 2779 - 2788. [Abstract] [PDF]
|
|
|
|
|
|
M de Cuevas, J. Lee, and A. Spradling alpha-spectrin is required for germline cell division and differentiation in the Drosophila ovary Development, January 12, 1996; 122(12): 3959 - 3968. [Abstract] [PDF]
|
|
|
|
|
|
S Staab, A Heller, and M Steinmann-Zwicky Somatic sex-determining signals act on XX germ cells in Drosophila embryos Development, January 12, 1996; 122(12): 4065 - 4071. [Abstract] [PDF]
|
|
|
|
|
|
K. Clark and D. McKearin The Drosophila stonewall gene encodes a putative transcription factor essential for germ cell development Development, January 3, 1996; 122(3): 937 - 950. [Abstract] [PDF]
|
|
|
|
|
|
N. Hawkins, J Thorpe, and T Schupbach Encore, a gene required for the regulation of germ line mitosis and oocyte differentiation during Drosophila oogenesis Development, January 1, 1996; 122(1): 281 - 290. [Abstract] [PDF]
|
|
