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Development, Vol 121, Issue 9 2961-2972, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
S Strome, P Martin, E Schierenberg and J Paulsen
Department of Biology, Indiana University, Bloomington 47405, USA.
Mutations in the maternal-effect sterile gene mes-1 cause the offspring of homozygous mutant mothers to develop into sterile adults. Lineage analysis revealed that mutant offspring are sterile because they fail to form primordial germ cells during embryogenesis. In wild-type embryos, the primordial germ cell P4 is generated via a series of four unequal stem-cell divisions of the zygote. mes-1 embryos display a premature and progressive loss of polarity in these divisions: P0 and P1 undergo apparently normal unequal divisions and cytoplasmic partitioning, but P2 (in some embryos) and P3 (in most embryos) display defects in cleavage asymmetry and fail to partition lineage-specific components to only one daughter cell. As an apparent consequence of these defects, P4 is transformed into a muscle precursor, like its somatic sister cell D, and generates up to 20 body muscle cells instead of germ cells. Our results show that the wild-type mes-1 gene participates in promoting unequal germ-line divisions and asymmetric partitioning events and thus the determination of cell fate in early C. elegans embryos.
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G Seydoux and S Strome Launching the germline in Caenorhabditis elegans: regulation of gene expression in early germ cells Development, January 8, 1999; 126(15): 3275 - 3283. [Abstract] [PDF]
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F. Pickett, M. Champagne, and D. Meeks-Wagner Temperature-sensitive mutations that arrest Arabidopsis shoot development Development, January 12, 1996; 122(12): 3799 - 3807. [Abstract] [PDF]
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S. Hird, J. Paulsen, and S Strome Segregation of germ granules in living Caenorhabditis elegans embryos: cell-type-specific mechanisms for cytoplasmic localisation Development, January 4, 1996; 122(4): 1303 - 1312. [Abstract] [PDF]
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