|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 122, Issue 10 3141-3150, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
ST Smith and JB Jaynes
Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107, USA.
The engrailed homeoprotein is a dominantly acting or 'active' transcriptional repressor both in cultured cells and in vivo. When retargeted via a homeodomain swap to the endogenous fushi tarazu gene (ftz), it actively represses it, resulting in a ftz mutant phenocopy. We have mapped functional regions of engrailed using this in vivo repression assay. In addition to a region containing an active repression domain identified in cell culture assays (K. Han and J. L. Manley (1993) EMBO J. 12, 2723-2733), we find that two evolutionarily conserved regions contribute to activity. The one of these that does not flank the HD is particularly crucial to repression activity in vivo. We find that this domain is present not only in all engrailed-class homeoproteins but also in all known members of several other classes, including goosecoid, Nk1, Nk2 and msh. Thus engrailed's active repression function in vivo is dependent on a highly conserved interaction that was established early in the evolution of the homeobox gene superfamily. We further show using rescue transgenes that the widely conserved in vivo repression domain is required for the normal function of engrailed in the embryo.
This article has been cited by other articles:
|
|
 |
|
  E. Peden, E. Kimberly, K. Gengyo-Ando, S. Mitani, and D. Xue Control of sex-specific apoptosis in C. elegans by the BarH homeodomain protein CEH-30 and the transcriptional repressor UNC-37/Groucho Genes & Dev., December 1, 2007; 21(23): 3195 - 3207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. F. Farin, M. Bussen, M. K. Schmidt, M. K. Singh, K. Schuster-Gossler, and A. Kispert Transcriptional Repression by the T-box Proteins Tbx18 and Tbx15 Depends on Groucho Corepressors J. Biol. Chem., August 31, 2007; 282(35): 25748 - 25759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Soustelle, F. Trousse, C. Jacques, J. Ceron, P. Cochard, C. Soula, and A. Giangrande Neurogenic role of Gcm transcription factors is conserved in chicken spinal cord Development, February 1, 2007; 134(3): 625 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yaklichkin, A. B. Steiner, Q. Lu, and D. S. Kessler FoxD3 and Grg4 Physically Interact to Repress Transcription and Induce Mesoderm in Xenopus J. Biol. Chem., January 26, 2007; 282(4): 2548 - 2557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Heimbucher, C. Murko, B. Bajoghli, N. Aghaallaei, A. Huber, R. Stebegg, D. Eberhard, M. Fink, A. Simeone, and T. Czerny Gbx2 and Otx2 Interact with the WD40 Domain of Groucho/Tle Corepressors Mol. Cell. Biol., January 1, 2007; 27(1): 340 - 351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bergsland, M. Werme, M. Malewicz, T. Perlmann, and J. Muhr The establishment of neuronal properties is controlled by Sox4 and Sox11 Genes & Dev., December 15, 2006; 20(24): 3475 - 3486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Ratnaparkhi, S. Jia, and A. J. Courey Uncoupling Dorsal-mediated activation from Dorsal-mediated repression in the Drosophila embryo Development, November 15, 2006; 133(22): 4409 - 4414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Nakada, S. Satoh, Y. Tabata, K.-i. Arai, and S. Watanabe Transcriptional Repressor foxl1 Regulates Central Nervous System Development by Suppressing shh Expression in Zebra Fish. Mol. Cell. Biol., October 1, 2006; 26(19): 7246 - 7257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Goldstein, O. Cook, T. Dinur, A. Pisante, U. C. Karandikar, A. Bidwai, and Z. Paroush An eh1-Like Motif in Odd-skipped Mediates Recruitment of Groucho and Repression In Vivo Mol. Cell. Biol., December 15, 2005; 25(24): 10711 - 10720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Hittinger, D. L. Stern, and S. B. Carroll Pleiotropic functions of a conserved insect-specific Hox peptide motif Development, December 1, 2005; 132(23): 5261 - 5270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Chen, L. R. Schaevitz, and S. K. McConnell Fezl regulates the differentiation and axon targeting of layer 5 subcortical projection neurons in cerebral cortex PNAS, November 22, 2005; 102(47): 17184 - 17189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Saba, J. E. Johnson, and T. Saito Commissural neuron identity is specified by a homeodomain protein, Mbh1, that is directly downstream of Math1 Development, May 1, 2005; 132(9): 2147 - 2155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. Schubert and A. Lumsden Transcriptional control of early tract formation in the embryonic chick midbrain Development, April 15, 2005; 132(8): 1785 - 1793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Offner, N. Duval, M. Jamrich, and B. Durand The pro-apoptotic activity of a vertebrate Bar-like homeobox gene plays a key role in patterning the Xenopus neural plate by limiting the number of chordin- and shh-expressing cells Development, April 15, 2005; 132(8): 1807 - 1818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Muller, K. Anlag, H. Wildner, S. Britsch, M. Treier, and C. Birchmeier The bHLH factor Olig3 coordinates the specification of dorsal neurons in the spinal cord Genes & Dev., March 15, 2005; 19(6): 733 - 743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Olson, J. Zhang, H. Taylor, D. W. Rose, and M. G. Rosenfeld Barx2 functions through distinct corepressor classes to regulate hair follicle remodeling PNAS, March 8, 2005; 102(10): 3708 - 3713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Yu, L.-J. Syu, and D. M. Mellerick Contextual interactions determine whether the Drosophila homeodomain protein, Vnd, acts as a repressor or activator Nucleic Acids Res., January 7, 2005; 33(1): 1 - 12. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Stepchenko and M. Nirenberg Mapping activation and repression domains of the vnd/NK-2 homeodomain protein PNAS, September 7, 2004; 101(36): 13180 - 13185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Swingler, K. L. Bess, J. Yao, S. Stifani, and P.-S. Jayaraman The Proline-rich Homeodomain Protein Recruits Members of the Groucho/Transducin-like Enhancer of Split Protein Family to Co-repress Transcription in Hematopoietic Cells J. Biol. Chem., August 13, 2004; 279(33): 34938 - 34947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ben Abdelkhalek, A. Beckers, K. Schuster-Gossler, M. N. Pavlova, H. Burkhardt, H. Lickert, J. Rossant, R. Reinhardt, L. C. Schalkwyk, I. Muller, et al. The mouse homeobox gene Not is required for caudal notochord development and affected by the truncate mutation Genes & Dev., July 15, 2004; 18(14): 1725 - 1736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Petropoulos, P. J. Gianakopoulos, A. G. Ridgeway, and I. S. Skerjanc Disruption of Meox or Gli Activity Ablates Skeletal Myogenesis in P19 Cells J. Biol. Chem., June 4, 2004; 279(23): 23874 - 23881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nakada, T. L. Hunsaker, R. M. Henke, and J. E. Johnson Distinct domains within Mash1 and Math1 are required for function in neuronal differentiation versus neuronal cell-type specification Development, March 15, 2004; 131(6): 1319 - 1330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Simon and T. Lufkin Postnatal Lethality in Mice Lacking the Sax2 Homeobox Gene Homologous to Drosophila S59/slouch: Evidence for Positive and Negative Autoregulation Mol. Cell. Biol., December 15, 2003; 23(24): 9046 - 9060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lim and K.-W. Choi Bar homeodomain proteins are anti-proneural in the Drosophila eye: transcriptional repression of atonal by Bar prevents ectopic retinal neurogenesis Development, December 15, 2003; 130(24): 5965 - 5974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Brinkmeier, M. A. Potok, K. B. Cha, T. Gridley, S. Stifani, J. Meeldijk, H. Clevers, and S. A. Camper TCF and Groucho-Related Genes Influence Pituitary Growth and Development Mol. Endocrinol., November 1, 2003; 17(11): 2152 - 2161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Leung, J. Bischof, I. Soll, D. Niessing, D. Zhang, J. Ma, H. Jackle, and W. Driever bozozok directly represses bmp2b transcription and mediates the earliest dorsoventral asymmetry of bmp2b expression in zebrafish Development, August 15, 2003; 130(16): 3639 - 3649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Klinedinst and R. Bodmer Gata factor Pannier is required to establish competence for heart progenitor formation Development, July 1, 2003; 130(13): 3027 - 3038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-K. Bae, T. Shimizu, T. Yabe, C.-H. Kim, T. Hirata, H. Nojima, O. Muraoka, T. Hirano, and M. Hibi A homeobox gene, pnx, is involved in the formation of posterior neurons in zebrafish Development, May 1, 2003; 130(9): 1853 - 1865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. William, Y. Tanabe, and T. M. Jessell Regulation of motor neuron subtype identity by repressor activity of Mnx class homeodomain proteins Development, April 15, 2003; 130(8): 1523 - 1536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Tepera, P. D. McCrea, and J. M. Rosen A {beta}-catenin survival signal is required for normal lobular development in the mammary gland J. Cell Sci., March 15, 2003; 116(6): 1137 - 1149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Zhu, M. A. Dyer, M. Uchikawa, H. Kondoh, O. V. Lagutin, and G. Oliver Six3-mediated auto repression and eye development requires its interaction with members of the Groucho-related family of co-repressors Development, March 8, 2003; 129(12): 2835 - 2849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Alexandre and J.-P. Vincent Requirements for transcriptional repression and activation by Engrailed in Drosophila embryos Development, February 15, 2003; 130(4): 729 - 739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kobayashi, M. Fujioka, E. N. Tolkunova, D. Deka, M. Abu-Shaar, R. S. Mann, and J. B. Jaynes Engrailed cooperates with extradenticle and homothorax to repress target genes in Drosophila Development, February 15, 2003; 130(4): 741 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Olson, J. S. Dasen, B. Gun Ju, J. Tollkuhn, and M. G. Rosenfeld Paired-like Repression/Activation in Pituitary Development Recent Prog. Horm. Res., January 1, 2003; 58(1): 249 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Barolo, T. Stone, A. G. Bang, and J. W. Posakony Default repression and Notch signaling: Hairless acts as an adaptor to recruit the corepressors Groucho and dCtBP to Suppressor of Hairless Genes & Dev., August 1, 2002; 16(15): 1964 - 1976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Petropoulos and I. S. Skerjanc beta -Catenin Is Essential and Sufficient for Skeletal Myogenesis in P19 Cells J. Biol. Chem., May 3, 2002; 277(18): 15393 - 15399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.C. Lidral and B.C. Reising The Role of MSX1 in Human Tooth Agenesis J. Dent. Res., April 1, 2002; 81(4): 274 - 278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Scully and M. G. Rosenfeld Pituitary Development: Regulatory Codes in Mammalian Organogenesis Science, March 22, 2002; 295(5563): 2231 - 2235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fujioka, G. L. Yusibova, N. H. Patel, S. J. Brown, and J. B. Jaynes The repressor activity of Even-skipped is highly conserved, and is sufficient to activate engrailed and to regulate both the spacing and stability of parasegment boundaries Development, January 10, 2002; 129(19): 4411 - 4421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Brickman, M. Clements, R. Tyrell, D. McNay, K. Woods, J. Warner, A. Stewart, R. S. P. Beddington, and M. Dattani Molecular effects of novel mutations in Hesx1/HESX1 associated with human pituitary disorders Development, December 15, 2001; 128(24): 5189 - 5199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Dasen, J.-P. M. Barbera, T. S. Herman, S. O' Connell, L. Olson, B. Ju, J. Tollkuhn, S. H. Baek, D. W. Rose, and M. G. Rosenfeld Temporal regulation of a paired-like homeodomain repressor/TLE corepressor complex and a related activator is required for pituitary organogenesis Genes & Dev., December 1, 2001; 15(23): 3193 - 3207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yasuo and P. Lemaire Role of Goosecoid, Xnot and Wnt antagonists in the maintenance of the notochord genetic programme in Xenopus gastrulae Development, October 1, 2001; 128(19): 3783 - 3793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Kobayashi, R. Goldstein, M Fujioka, Z Paroush, and J. Jaynes Groucho augments the repression of multiple Even skipped target genes in establishing parasegment boundaries Development, January 5, 2001; 128(10): 1805 - 1815. [Abstract] [PDF]
|
|
|
|
|
|
R. Flores-Saaib, S Jia, and A. Courey Activation and repression by the C-terminal domain of Dorsal Development, January 5, 2001; 128(10): 1869 - 1879. [Abstract] [PDF]
|
|
|
|
|
|
M. S. Lechner, I. Levitan, and G. R. Dressler PTIP, a novel BRCT domain-containing protein interacts with Pax2 and is associated with active chromatin Nucleic Acids Res., July 15, 2000; 28(14): 2741 - 2751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Montross, H Ji, and P. McCrea A beta-catenin/engrailed chimera selectively suppresses Wnt signaling J. Cell Sci., May 1, 2000; 113(10): 1759 - 1770. [Abstract] [PDF]
|
|
|
|
|
|
H. Lee and M Frasch Wingless effects mesoderm patterning and ectoderm segmentation events via induction of its downstream target sloppy paired Development, January 12, 2000; 127(24): 5497 - 5508. [Abstract] [PDF]
|
|
|
|
|
|
K Sawada, Y Konishi, M Tominaga, Y Watanabe, J Hirano, S Inoue, R Kageyama, M Blum, and A Tominaga Goosecoid suppresses cell growth and enhances neuronal differentiation of PC12 cells J. Cell Sci., January 8, 2000; 113(15): 2705 - 2713. [Abstract] [PDF]
|
|
|
|
|
|
A. Gershon, J Rudnick, L Kalam, and K Zimmerman The homeodomain-containing gene Xdbx inhibits neuronal differentiation in the developing embryo Development, January 7, 2000; 127(13): 2945 - 2954. [Abstract] [PDF]
|
|
|
|
|
|
J. Brickman, C. Jones, M Clements, J. Smith, and R. Beddington Hex is a transcriptional repressor that contributes to anterior identity and suppresses Spemann organiser function Development, January 6, 2000; 127(11): 2303 - 2315. [Abstract] [PDF]
|
|
|
|
|
|
E Matsunaga, I Araki, and H Nakamura Pax6 defines the di-mesencephalic boundary by repressing En1 and Pax2 Development, January 6, 2000; 127(11): 2357 - 2365. [Abstract] [PDF]
|
|
|
|
|
|
S. C. Tucker and R. Wisdom Site-specific Heterodimerization by Paired Class Homeodomain Proteins Mediates Selective Transcriptional Responses J. Biol. Chem., November 5, 1999; 274(45): 32325 - 32332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Winnier, J. Y.-J. Meir, J. M. Ross, N. Tavernarakis, M. Driscoll, T. Ishihara, I. Katsura, and D. M. Miller III UNC-4/UNC-37-dependent repression of motor neuron-specific genes controls synaptic choice in Caenorhabditis elegans Genes & Dev., November 1, 1999; 13(21): 2774 - 2786. [Abstract] [Full Text]
|
|
|
|
|
|
G. Jimenez, C. P. Verrijzer, and D. Ish-Horowicz A Conserved Motif in Goosecoid Mediates Groucho-Dependent Repression in Drosophila Embryos Mol. Cell. Biol., March 1, 1999; 19(3): 2080 - 2087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I Araki and H Nakamura Engrailed defines the position of dorsal di-mesencephalic boundary by repressing diencephalic fate Development, January 11, 1999; 126(22): 5127 - 5135. [Abstract] [PDF]
|
|
|
|
|
|
G. Ermakova, E. Alexandrova, O. Kazanskaya, O. Vasiliev, M. Smith, and A. Zaraisky The homeobox gene, Xanf-1, can control both neural differentiation and patterning in the presumptive anterior neurectoderm of the Xenopus laevis embryo Development, January 10, 1999; 126(20): 4513 - 4523. [Abstract] [PDF]
|
|
|
|
|
|
P. T. Schwartz and M. Vallejo Differential Regulation of Basal and Cyclic Adenosine 3',5'-Monophosphate-Induced Somatostatin Gene Transcription in Neural Cells by DNA Control Elements That Bind Homeodomain Proteins Mol. Endocrinol., September 1, 1998; 12(9): 1280 - 1293. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Logan, R. J. T. Wingate, I. J. McKay, and A. Lumsden Tlx-1 and Tlx-3 Homeobox Gene Expression in Cranial Sensory Ganglia and Hindbrain of the Chick Embryo: Markers of Patterned Connectivity J. Neurosci., July 15, 1998; 18(14): 5389 - 5402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Fisher and M. Caudy Groucho proteins: transcriptional corepressors for specific subsets of DNA-binding transcription factors in vertebrates and invertebrates Genes & Dev., July 1, 1998; 12(13): 1931 - 1940. [Full Text]
|
|
|
|
|
|
E. N. Tolkunova, M. Fujioka, M. Kobayashi, D. Deka, and J. B. Jaynes Two Distinct Types of Repression Domain in Engrailed: One Interacts with the Groucho Corepressor and Is Preferentially Active on Integrated Target Genes Mol. Cell. Biol., May 1, 1998; 18(5): 2804 - 2814. [Abstract] [Full Text]
|
|
|
|
|
|
M. Hanks, C. Loomis, E Harris, C. Tong, L Anson-Cartwright, A Auerbach, and A Joyner Drosophila engrailed can substitute for mouse Engrailed1 function in mid-hindbrain, but not limb development Development, January 11, 1998; 125(22): 4521 - 4530. [Abstract] [PDF]
|
|
|
|
|
|
G Ranganayakulu, D. Elliott, R. Harvey, and E. Olson Divergent roles for NK-2 class homeobox genes in cardiogenesis in flies and mice Development, January 8, 1998; 125(16): 3037 - 3048. [Abstract] [PDF]
|
|
|
|
|
|
S Dietrich, F. Schubert, C Healy, P. Sharpe, and A Lumsden Specification of the hypaxial muscula |
