|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 122, Issue 3 771-781, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
ME Mangan and JB Olmsted
Department of Biology, University of Rochester, NY 14627, USA.
Microtubule-associated protein 4 (MAP4) transcripts vary in different mouse tissues, with striated muscle (skeletal and cardiac) expressing 8- and 9-kb transcripts preferentially to the more widely distributed 5.5- and 6.5-kb transcripts (West, R. W., Tenbarge, K. M. and Olmsted, J. B. (1991). J. Biol. Chem. 266, 21886-21896). Cloning of the sequence unique to the muscle transcripts demonstrated that these mRNAs vary from the more ubiquitous ones by a single 3.2-kb coding region insertion within the projection domain of MAP4. During differentiation of the myogenic cell line, C2C12, muscle-specific MAP4 transcripts appear within 24 hours of growth in differentiation medium, and a larger MAP4 isotype (350 X 10(3) Mr) accumulates to high levels by 48 hours of differentiation. In situ hybridization analyses of transcript distribution in mouse embryos demonstrated that muscle-specific transcripts appear early in myogenesis. To block the expression of the muscle-specific MAP4, stable lines of C2C12 were generated bearing an antisense construct with the muscle-specific MAP4 sequence. Myoblast growth was unaffected whereas myotube formation was severely perturbed. Fusion occurred in the absence of the muscle MAP4 isotype, but the multinucleate syncytia were short and apolar, microtubules were disorganized and normal anisotropic myofibrils were absent. The patterns of expression of the muscle-specific transcripts and the antisense experiments indicated that this unique structural form of MAP4 plays a critical role in the formation and maintenance of muscle.
This article has been cited by other articles:
|
|
 |
|
  A. S. McElhinny, C. N. Perry, C. C. Witt, S. Labeit, and C. C. Gregorio Muscle-specific RING finger-2 (MURF-2) is important for microtubule, intermediate filament and sarcomeric M-line maintenance in striated muscle development J. Cell Sci., July 1, 2004; 117(15): 3175 - 3188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Takahashi, H. Shiraishi, Y. Ishibashi, K. L. Blade, P. J. McDermott, D. R. Menick, D. Kuppuswamy, and G. Cooper IV Phenotypic consequences of {beta}1-tubulin expression and MAP4 decoration of microtubules in adult cardiocytes Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H2072 - H2083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Volonte, A. J. Peoples, and F. Galbiati Modulation of Myoblast Fusion by Caveolin-3 in Dystrophic Skeletal Muscle Cells: Implications for Duchenne Muscular Dystrophy and Limb-Girdle Muscular Dystrophy-1C Mol. Biol. Cell, October 1, 2003; 14(10): 4075 - 4088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Ginkel and L. Wordeman Expression and Partial Characterization of Kinesin-related Proteins in Differentiating and Adult Skeletal Muscle Mol. Biol. Cell, December 1, 2000; 11(12): 4143 - 4158. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. A. Spencer, S. Eliazer, R. L. Ilaria , Jr., J. A. Richardson, and E. N. Olson Regulation of Microtubule Dynamics and Myogenic Differentiation by MURF, a Striated Muscle RING-Finger Protein J. Cell Biol., August 21, 2000; 150(4): 771 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ralston, Z. Lu, and T. Ploug The Organization of the Golgi Complex and Microtubules in Skeletal Muscle Is Fiber Type-Dependent J. Neurosci., December 15, 1999; 19(24): 10694 - 10705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Narishige, K. L. Blade, Y. Ishibashi, T. Nagai, M. Hamawaki, D. R. Menick, D. Kuppuswamy, and G. Cooper IV Cardiac Hypertrophic and Developmental Regulation of the beta -Tubulin Multigene Family J. Biol. Chem., April 2, 1999; 274(14): 9692 - 9697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nguyen, D Gruber, and J. Bulinski Microtubule-associated protein 4 (MAP4) regulates assembly, protomer-polymer partitioning and synthesis of tubulin in cultured cells J. Cell Sci., January 6, 1999; 112(12): 1813 - 1824. [Abstract] [PDF]
|
|
|
|
|
|
U Kaufmann, J Kirsch, A Irintchev, A Wernig, and A Starzinski-Powitz The M-cadherin catenin complex interacts with microtubules in skeletal muscle cells: implications for the fusion of myoblasts J. Cell Sci., January 1, 1999; 112(1): 55 - 68. [Abstract] [PDF]
|
|
|
|
|
|
H. Sato, T. Nagai, D. Kuppuswamy, T. Narishige, M. Koide, D. R. Menick, and G. C. IV Microtubule Stabilization in Pressure Overload Cardiac Hypertrophy J. Cell Biol., November 17, 1997; 139(4): 963 - 973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S.L. Andersen and E. Karsenti XMAP310: A Xenopus Rescue-promoting Factor Localized to the Mitotic Spindle J. Cell Biol., November 17, 1997; 139(4): 975 - 983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nguyen, S Chari, D Gruber, C. Lue, S. Chapin, and J. Bulinski Overexpression of full- or partial-length MAP4 stabilizes microtubules and alters cell growth J. Cell Sci., January 1, 1997; 110(2): 281 - 294. [Abstract] [PDF]
|
|
