|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 122, Issue 8 2485-2496, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
JJ Stachecki and DR Armant
Department of Obstetrics and Gynecology, C.S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Inositol 1,4,5-trisphosphate can regulate growth and differentiation by modulating the release of intracellular Ca2+ in a variety of cellular systems, and it is involved in oocyte activation. Recent studies suggest that mammalian preimplantation development may also be regulated by the release of Ca2+ from intracellular stores. The rate of cavitation and cell division was accelerated after a transient elevation of intracellular Ca2+ levels was induced in morulae by exposure to ethanol or ionomycin. Embryos exposed to BAPTA-AM, a chelator of intracellular Ca2+, exhibited a brief dose-dependent reduction in basal Ca2+ levels, a temporal inhibition of ionophore-induced Ca2+ signalling and a subsequent delay in blastocoel formation. BAPTA-AM at 0.5 microM did not significantly alter the basal intracellular calcium level, but chelated Ca2+ that was released after ethanol exposure and thereby attenuated the ethanol-induced acceleration of cavitation. BAPTA-AM also inhibited cell division to the 16-cell stage in a dose-dependent manner, which correlated with the inhibition of cavitation. Thimerosal and inositol 1,4,5-trisphosphate significantly elevated the intracellular Ca2+ concentration in mouse morula-stage embryos, providing evidence for the existence of inositol 1,4,5-trisphosphate-sensitive Ca2+ stores. Although caffeine failed to release intracellular Ca2+, ryanodine induced a small biphasic release of Ca2+, suggesting that ryanodine-sensitive Ca2+ stores may also exist in mouse embryos. Morulae exposed to the calmodulin inhibitor W-7 exhibited a dose-dependent delay in blastocoel formation. A 4 hour exposure to 10 microM W-7 did not significantly alter cavitation, but attenuated the ionophore-induced stimulation of blastocoel formation. This finding suggests that the developmental effects produced through Ca2+ signalling are mediated by calmodulin. Our results demonstrate that Ca2+ release in mouse morulae occurs predominantly through the inositol 1,4,5-trisphosphate receptor, and that alteration of intracellular Ca2+ levels can accelerate or delay embryonic growth and differentiation, providing a mechanistic link between the regulation of oocyte and embryonic development.
This article has been cited by other articles:
|
|
 |
|
  T. Hamatani, T. Daikoku, H. Wang, H. Matsumoto, M. G. Carter, M. S. H. Ko, and S. K. Dey Global gene expression analysis identifies molecular pathways distinguishing blastocyst dormancy and activation PNAS, July 13, 2004; 101(28): 10326 - 10331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J Eckert, A. McCallum, A. Mears, M. G Rumsby, I. T Cameron, and T. P Fleming PKC signalling regulates tight junction membrane assembly in the pre-implantation mouse embryo Reproduction, June 1, 2004; 127(6): 653 - 667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. T.Y. Chiu, M. S. Rogers, E. L.K. Law, C. M. Briton-Jones, L.P. Cheung, and C. J. Haines Follicular fluid and serum concentrations of myo-inositol in patients undergoing IVF: relationship with oocyte quality Hum. Reprod., June 1, 2002; 17(6): 1591 - 1596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Biggers and L. K. McGinnis Evidence that glucose is not always an inhibitor of mouse preimplantation development in vitro Hum. Reprod., January 1, 2001; 16(1): 153 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Wang, L Mayernik, J. Schultz, and D. Armant Acceleration of trophoblast differentiation by heparin-binding EGF-like growth factor is dependent on the stage-specific activation of calcium influx by ErbB receptors in developing mouse blastocysts Development, January 1, 2000; 127(1): 33 - 44. [Abstract] [PDF]
|
|
|
|
 |
|
 P.T. Goud, A.P. Goud, P. Van Oostveldt, and M. Dhont Presence and dynamic redistribution of type I inositol 1,4,5-trisphosphate receptors in human oocytes and embryos during in-vitro maturation, fertilization and early cleavage divisions Mol. Hum. Reprod., May 1, 1999; 5(5): 441 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang, B. C. Paria, S. K. Dey, and D. R. Armant Stage-Specific Excitation of Cannabinoid Receptor Exhibits Differential Effects on Mouse Embryonic Development Biol Reprod, April 1, 1999; 60(4): 839 - 844. [Abstract] [Full Text]
|
|
|
|
|
|
T. Stojanov and C. O'Neill Ontogeny of Expression of a Receptor for Platelet-Activating Factor in Mouse Preimplantation Embryos and the Effects of Fertilization and Culture In Vitro on Its Expression Biol Reprod, March 1, 1999; 60(3): 674 - 682. [Abstract] [Full Text]
|
|
|
|
|
|
R. Pey, C. Vial, G. Schatten, and M. Hafner Increase of intracellular Ca2+ and relocation of E-cadherin during experimental decompaction of mouse embryos PNAS, October 27, 1998; 95(22): 12977 - 12982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Wang, U. Rout, I. Bagchi, and D. Armant Expression of calcitonin receptors in mouse preimplantation embryos and their function in the regulation of blastocyst differentiation by calcitonin Development, January 11, 1998; 125(21): 4293 - 4302. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Emerson, A. R. Travis, R. Bathgate, T. Stojanov, D. I. Cook, E. Harding, D. P. Lu, and C. O'Neill Characterization and Functional Significance of Calcium Transients in the 2-Cell Mouse Embryo Induced by an Autocrine Growth Factor J. Biol. Chem., July 14, 2000; 275(29): 21905 - 21913. [Abstract] [Full Text] [PDF]
|
|
