|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 124, Issue 14 2769-2780, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
KM Downs and C Harmann
Department of Anatomy, University of Wisconsin-Madison Medical School 53706, USA. kdowns@facstaff.wisc.edu
The murine allantois is the future umbilical component of the placenta. The base of the allantois is also thought to contain the future germ line. We have examined the fate and developmental potency of cells within the murine allantois during gastrulation. lacZ-expressing headfold-stage allantoises (approximately 8.0 days postcoitum; dpc) were subdivided into three proximodistal regions and transplanted into three sites in synchronous non-transgenic host embryos: the primitive streak at the level of prospective paraxial mesoderm, the primitive streak at the level of lateral plate mesoderm, and the base of the allantois. After 23 hours in culture, operated conceptuses were examined histologically for contribution of donor allantoic cells to the conceptus. None of the allantoic regions contributed to paraxial mesoderm when placed into the fetus, but all three colonized the endothelium and adjacent mesenchyme of the dorsal aorta. The mid-region was most efficient at colonizing endothelium, whereas the base was the only allantoic region to exhibit relative pluripotency, colonizing several derivatives of all three primary germ layers. Differences in the state of differentiation along the proximodistal axis of the allantois were further borne out when the three allantoic regions were placed into the base of the allantois of host conceptuses. Striking differences were observed in final position along the proximodistal axis of the host allantois. Most grafted cells translocated distally from the base; however, basal donor allantoic cells translocated typically only as far as the host's mid-region, whereas donor allantoic tip cells typically returned to the tip, often colonizing the chorioallantoic fusion junction. Together, our data reveal that the headfold-stage allantois may contain a proximodistal gradient of differentiation, and raise intriguing questions about how this gradient was established and the role it plays in umbilical vasculogenesis.
This article has been cited by other articles:
|
|
 |
|
  B. M. Zeigler, D. Sugiyama, M. Chen, Y. Guo, K. M. Downs, and N. A. Speck The allantois and chorion, when isolated before circulation or chorio-allantoic fusion, have hematopoietic potential Development, November 1, 2006; 133(21): 4183 - 4192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. K. A. Mikkola and S. H. Orkin The journey of developing hematopoietic stem cells. Development, October 1, 2006; 133(19): 3733 - 3744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Inman and K. M. Downs Brachyury is required for elongation and vasculogenesis in the murine allantois Development, August 1, 2006; 133(15): 2947 - 2959. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.E. Ferguson III, R. W. Kelley, and C. Patterson Mechanisms of Endothelial Differentiation in Embryonic Vasculogenesis Arterioscler. Thromb. Vasc. Biol., November 1, 2005; 25(11): 2246 - 2254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Downs, E. R. Hellman, J. McHugh, K. Barrickman, and K. E. Inman Investigation into a role for the primitive streak in development of the murine allantois Development, January 1, 2004; 131(1): 37 - 55. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Alvarez-Silva, P. Belo-Diabangouaya, J. Salaun, and F. Dieterlen-Lievre Mouse placenta is a major hematopoietic organ Development, November 15, 2003; 130(22): 5437 - 5444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Uy, K. M. Downs, and R. L. Gardner Inhibition of trophoblast stem cell potential in chorionic ectoderm coincides with occlusion of the ectoplacental cavity in the mouse Development, March 10, 2003; 129(16): 3913 - 3924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Fujiwara, N. R. Dunn, and B. L. M. Hogan Bone morphogenetic protein 4 in the extraembryonic mesoderm is required for allantois development and the localization and survival of primordial germ cells in the mouse PNAS, November 9, 2001; (2001) 241508898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Redick and V. L. Bautch Developmental Platelet Endothelial Cell Adhesion Molecule Expression Suggests Multiple Roles for a Vascular Adhesion Molecule Am. J. Pathol., April 1, 1999; 154(4): 1137 - 1147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kinder, T. Tsang, G. Quinlan, A. Hadjantonakis, A Nagy, and P. Tam The orderly allocation of mesodermal cells to the extraembryonic structures and the anteroposterior axis during gastrulation of the mouse embryo Development, January 11, 1999; 126(21): 4691 - 4701. [Abstract] [PDF]
|
|
|
|
|
|
S Gory-Faure, M. Prandini, H Pointu, V Roullot, I Pignot-Paintrand, M Vernet, and P Huber Role of vascular endothelial-cadherin in vascular morphogenesis Development, January 5, 1999; 126(10): 2093 - 2102. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Gory, M. Vernet, M. Laurent, E. Dejana, J. Dalmon, and P. Huber The Vascular Endothelial-Cadherin Promoter Directs Endothelial-Specific Expression in Transgenic Mice Blood, January 1, 1999; 93(1): 184 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Downs, S Gifford, M Blahnik, and R. Gardner Vascularization in the murine allantois occurs by vasculogenesis without accompanying erythropoiesis Development, January 11, 1998; 125(22): 4507 - 4520. [Abstract] [PDF]
|
|
|
|
|
|
T. Fujiwara, N. R. Dunn, and B. L. M. Hogan Bone morphogenetic protein 4 in the extraembryonic mesoderm is required for allantois development and the localization and survival of primordial germ cells in the mouse PNAS, November 20, 2001; 98(24): 13739 - 13744. [Abstract] [Full Text] [PDF]
|
|
