|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 124, Issue 16 3157-3165, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
C Yoon, K Kawakami and N Hopkins
Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA.
Identification and manipulation of the germ line are important to the study of model organisms. Although zebrafish has recently emerged as a model for vertebrate development, the primordial germ cells (PGCs) in this organism have not been previously described. To identify a molecular marker for the zebrafish PGCs, we cloned the zebrafish homologue of the Drosophila vasa gene, which, in the fly, encodes a germ-cell-specific protein. Northern blotting revealed that zebrafish vasa homologue (vas) transcript is present in embryos just after fertilization, and hence it is probably maternally supplied. Using whole-mount in situ hybridization, we investigated the expression pattern of vas RNA in zebrafish embryos from the 1-cell stage to 10 days of development. Here we present evidence that vas RNA is a germ-cell-specific marker, allowing a description of the zebrafish PGCs for the first time. Furthermore, vas transcript was detected in a novel pattern, localized to the cleavage planes in 2- and 4-cell-stage embryos. During subsequent cleavages, the RNA is segregated as subcellular clumps to a small number of cells that may be the future germ cells. These results suggest new ways in which one might develop techniques for the genetic manipulation of zebrafish. Furthermore, they provide the basis for further studies on this novel RNA localization pattern and on germ-line development in general.
This article has been cited by other articles:
|
|
 |
|
  J. Rodriguez-Leon, C. R. Esteban, M. Marti, B. Santiago-Josefat, I. Dubova, X. Rubiralta, and J. C. I. Belmonte Pitx2 regulates gonad morphogenesis PNAS, August 12, 2008; 105(32): 11242 - 11247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yano, K. Suzuki, and G. Yoshizaki Flow-Cytometric Isolation of Testicular Germ Cells from Rainbow Trout (Oncorhynchus mykiss) Carrying the Green Fluorescent Protein Gene Driven by Trout vasa Regulatory Regions Biol Reprod, January 1, 2008; 78(1): 151 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. M. Extavour Evolution of the bilaterian germ line: lineage origin and modulation of specification mechanisms Integr. Comp. Biol., November 1, 2007; 47(5): 770 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Nakamura, Y. Yamamoto, F. Usui, T. Mushika, T. Ono, A. R. Setioko, K. Takeda, K. Nirasawa, H. Kagami, and T. Tagami Migration and Proliferation of Primordial Germ Cells in the Early Chicken Embryo Poult. Sci., October 1, 2007; 86(10): 2182 - 2193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Urven, T. Yabe, and F. Pelegri A role for non-muscle myosin II function in furrow maturation in the early zebrafish embryo J. Cell Sci., October 15, 2006; 119(20): 4342 - 4352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Thermes, E. Candal, A. Alunni, G. Serin, F. Bourrat, and J.-S. Joly Medaka simplet (FAM53B) belongs to a family of novel vertebrate genes controlling cell proliferation Development, May 15, 2006; 133(10): 1881 - 1890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Blaser, S. Eisenbeiss, M. Neumann, M. Reichman-Fried, B. Thisse, C. Thisse, and E. Raz Transition from non-motile behaviour to directed migration during early PGC development in zebrafish J. Cell Sci., September 1, 2005; 118(17): 4027 - 4038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Yoshizaki, Y. Tago, Y. Takeuchi, E. Sawatari, T. Kobayashi, and T. Takeuchi Green Fluorescent Protein Labeling of Primordial Germ Cells Using a Nontransgenic Method and Its Application for Germ Cell Transplantation in Salmonidae Biol Reprod, July 1, 2005; 73(1): 88 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-T. Wong, Y. Gothilf, N. Zmora, K. E. Kight, I. Meiri, A. Elizur, and Y. Zohar Developmental Expression of Three Forms of Gonadotropin-Releasing Hormone and Ontogeny of the Hypothalamic-Pituitary-Gonadal Axis in Gilthead Seabream (Sparus aurata) Biol Reprod, September 1, 2004; 71(3): 1026 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cardinali, G. Gioacchini, S. Candiani, M. Pestarino, G. Yoshizaki, and O. Carnevali Hormonal Regulation of Vasa-Like Messenger RNA Expression in the Ovary of the Marine Teleost Sparus aurata Biol Reprod, March 1, 2004; 70(3): 737 - 743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kuramochi-Miyagawa, T. Kimura, T. W. Ijiri, T. Isobe, N. Asada, Y. Fujita, M. Ikawa, N. Iwai, M. Okabe, W. Deng, et al. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis Development, February 15, 2004; 131(4): 839 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bakkers, C. Kramer, J. Pothof, N. E. M. Quaedvlieg, H. P. Spaink, and M. Hammerschmidt Has2 is required upstream of Rac1 to govern dorsal migration of lateral cells during zebrafish gastrulation Development, February 1, 2004; 131(3): 525 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Extavour and M. Akam Mechanisms of germ cell specification across the metazoans: epigenesis and preformation Development, December 15, 2003; 130(24): 5869 - 5884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. E. Bruce, C. Howley, Y. Zhou, S. L. Vickers, L. M. Silver, M. L. King, and R. K. Ho The maternally expressed zebrafish T-box gene eomesodermin regulates organizer formation Development, November 15, 2003; 130(22): 5503 - 5517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sakai Transmeiotic differentiation of zebrafish germ cells into functional sperm in culture Development, March 9, 2003; 129(14): 3359 - 3365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ciruna, G. Weidinger, H. Knaut, B. Thisse, C. Thisse, E. Raz, and A. F. Schier Production of maternal-zygotic mutant zebrafish by germ-line replacement PNAS, November 12, 2002; 99(23): 14919 - 14924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takeuchi, G. Yoshizaki, T. Kobayashi, and T. Takeuchi Mass Isolation of Primordial Germ Cells from Transgenic Rainbow Trout Carrying the Green Fluorescent Protein Gene Driven by the vasa Gene Promoter Biol Reprod, October 1, 2002; 67(4): 1087 - 1092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Thisse and L. I. Zon Organogenesis--Heart and Blood Formation from the Zebrafish Point of View Science, January 18, 2002; 295(5554): 457 - 462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Weidinger, U. Wolke, M. Koprunner, C. Thisse, B. Thisse, and E. Raz Regulation of zebrafish primordial germ cell migration by attraction towards an intermediate target Development, January 1, 2002; 129(1): 25 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Koprunner, C. Thisse, B. Thisse, and E. Raz A zebrafish nanos-related gene is essential for the development of primordial germ cells Genes & Dev., November 1, 2001; 15(21): 2877 - 2885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kishida and G. V. Callard Distinct Cytochrome P450 Aromatase Isoforms in Zebrafish (Danio rerio) Brain and Ovary Are Differentially Programmed and Estrogen Regulated during Early Development Endocrinology, February 1, 2001; 142(2): 740 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Alarcon and R. Elinson RNA anchoring in the vegetal cortex of the Xenopus oocyte J. Cell Sci., January 5, 2001; 114(9): 1731 - 1741. [Abstract] [PDF]
|
|
|
|
|
|
J. Schisa, J. Pitt, and J. Priess Analysis of RNA associated with P granules in germ cells of C. elegans adults Development, January 4, 2001; 128(8): 1287 - 1298. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Knaut, F. Pelegri, K. Bohmann, H. Schwarz, and C. Nusslein-Volhard Zebrafish vasa RNA but Not Its Protein Is a Component of the Germ Plasm and Segregates Asymmetrically before Germline Specification J. Cell Biol., May 15, 2000; 149(4): 875 - 888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Tanaka, Y. Toyooka, R. Akasu, Y. Katoh-Fukui, Y. Nakahara, R. Suzuki, M. Yokoyama, and T. Noce The mouse homolog of Drosophila Vasa is required for the development of male germ cells Genes & Dev., April 1, 2000; 14(7): 841 - 853. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. C. LEKVEN, K. A. HELDE, C. J. THORPE, R. ROOKE, and R. T. MOON Reverse genetics in zebrafish Physiol Genomics, March 14, 2000; 2(2): 37 - 48. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N Tsunekawa, M Naito, Y Sakai, T Nishida, and T Noce Isolation of chicken vasa homolog gene and tracing the origin of primordial germ cells Development, January 6, 2000; 127(12): 2741 - 2750. [Abstract] [PDF]
|
|
|
|
|
|
A. Amsterdam, S. Burgess, G. Golling, W. Chen, Z. Sun, K. Townsend, S. Farrington, M. Haldi, and N. Hopkins A large-scale insertional mutagenesis screen in zebrafish Genes & Dev., October 15, 1999; 13(20): 2713 - 2724. [Abstract] [Full Text]
|
|
|
|
 |
|
 R.G. Edwards and H. K. Beard Hypothesis: sex determination and germline formation are committed at the pronucleate stage in mammalian embryos Mol. Hum. Reprod., July 1, 1999; 5(7): 595 - 606. [Full Text] [PDF]
|
|
|
|
|
|
G Weidinger, U Wolke, M Koprunner, M Klinger, and E Raz Identification of tissues and patterning events required for distinct steps in early migration of zebrafish primordial germ cells Development, January 12, 1999; 126(23): 5295 - 5307. [Abstract] [PDF]
|
|
|
|
|
|
D. Parichy, J. Rawls, S. Pratt, T. Whitfield, and S. Johnson Zebrafish sparse corresponds to an orthologue of c-kit and is required for the morphogenesis of a subpopulation of melanocytes, but is not essential for hematopoiesis or primordial germ cell development Development, January 8, 1999; 126(15): 3425 - 3436. [Abstract] [PDF]
|
|
|
|
|
|
R Creton, J. Speksnijder, and L. Jaffe Patterns of free calcium in zebrafish embryos J. Cell Sci., January 6, 1998; 111(12): 1613 - 1622. [Abstract] [PDF]
|
|
|
|
|
|
P Tomancak, A Guichet, P Zavorszky, and A Ephrussi Oocyte polarity depends on regulation of gurken by Vasa Development, January 5, 1998; 125(9): 1723 - 1732. [Abstract] [PDF]
|
|
|
|
|
|
C. Moens, S. Cordes, M. Giorgianni, G. Barsh, and C. Kimmel Equivalence in the genetic control of hindbrain segmentation in fish and mouse Development, January 2, 1998; 125(3): 381 - 391. [Abstract] [PDF]
|
|
|
|
|
|
M. Tanaka, M. Kinoshita, D. Kobayashi, and Y. Nagahama Establishment of medaka (Oryzias latipes) transgenic lines with the expression of green fluorescent protein fluorescence exclusively in germ cells: A useful model to monitor germ cells in a live vertebrate PNAS, February 27, 2001; 98(5): 2544 - 2549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ma, L. Fan, R. Ganassin, N. Bols, and P. Collodi Production of zebrafish germ-line chimeras from embryo cell cultures PNAS, February 27, 2001; 98(5): 2461 - 2466. [Abstract] [Full Text] [PDF]
|
|
