|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 124, Issue 18 3535-3541, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
L Gabay, R Seger and BZ Shilo
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Receptor tyrosine kinases (RTKs) and the signaling cascades that they trigger play central roles in diverse developmental processes. We describe the capacity to follow the active state of these signaling pathways in situ. This is achieved by monitoring, with a specific monoclonal antibody, the distribution of the active, dual phosphorylated form of MAP kinase (ERK). A dynamic pattern is observed during embryonic and larval phases of Drosophila development, which can be attributed, to a large extent, to the known RTKs. This specific detection has enabled us to determine the time of receptor activation, visualize gradients and boundaries of activation, and postulate the distribution of active ligands. Since the antibody was raised against the phosphorylated form of a conserved ERK peptide containing the TEY motif, this approach is applicable to a wide spectrum of multicellular organisms.
This article has been cited by other articles:
|
|
 |
|
  K. Oishi, H. Zhang, W. J. Gault, C. J. Wang, C. C. Tan, I.-K. Kim, H. Ying, T. Rahman, N. Pica, M. Tartaglia, et al. Phosphatase-defective LEOPARD syndrome mutations in PTPN11 gene have gain-of-function effects during Drosophila development Hum. Mol. Genet., January 1, 2009; 18(1): 193 - 201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. I. Miura, J.-Y. Roignant, M. Wassef, and J. E. Treisman Myopic acts in the endocytic pathway to enhance signaling by the Drosophila EGF receptor Development, June 1, 2008; 135(11): 1913 - 1922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Cinnamon, A. Helman, R. Ben-Haroush Schyr, A. Orian, G. Jimenez, and Z. Paroush Multiple RTK pathways downregulate Groucho-mediated repression in Drosophila embryogenesis Development, March 1, 2008; 135(5): 829 - 837. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Hellman, J. Spector, J. Robinson, X. Zuo, S. Saunier, C. Antignac, J. W. Tobias, and J. H. Lipschutz Matrix Metalloproteinase 13 (MMP13) and Tissue Inhibitor of Matrix Metalloproteinase 1 (TIMP1), Regulated by the MAPK Pathway, Are Both Necessary for Madin-Darby Canine Kidney Tubulogenesis J. Biol. Chem., February 15, 2008; 283(7): 4272 - 4282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Estes, E. Fulkerson, and Y. Zhang Identification of Motifs That Are Conserved in 12 Drosophila Species and Regulate Midline Glia vs. Neuron Expression Genetics, February 1, 2008; 178(2): 787 - 799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nishimura, Y. Inoue, and S. Hayashi A wave of EGFR signaling determines cell alignment and intercalation in the Drosophila tracheal placode Development, December 1, 2007; 134(23): 4273 - 4282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Toledano-Katchalski, R. Nir, G. Volohonsky, and T. Volk Post-transcriptional repression of the Drosophila midkine and pleiotrophin homolog miple by HOW is essential for correct mesoderm spreading Development, October 1, 2007; 134(19): 3473 - 3481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Harris and S. K. Beckendorf Different Wnt signals act through the Frizzled and RYK receptors during Drosophila salivary gland migration Development, June 1, 2007; 134(11): 2017 - 2025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Picco, C. Hudson, and H. Yasuo Ephrin-Eph signalling drives the asymmetric division of notochord/neural precursors in Ciona embryos Development, April 15, 2007; 134(8): 1491 - 1497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Kuslak, J. L. Thielen, and P. C. Marker The mouse seminal vesicle shape mutation is allelic with Fgfr2 Development, February 1, 2007; 134(3): 557 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kamimura, T. Koyama, H. Habuchi, R. Ueda, M. Masu, K. Kimata, and H. Nakato Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling J. Cell Biol., September 11, 2006; 174(6): 773 - 778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cela and M. Llimargas Egfr is essential for maintaining epithelial integrity during tracheal remodelling in Drosophila Development, August 15, 2006; 133(16): 3115 - 3125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Oishi, K. Gaengel, S. Krishnamoorthy, K. Kamiya, I.-K. Kim, H. Ying, U. Weber, L. A. Perkins, M. Tartaglia, M. Mlodzik, et al. Transgenic Drosophila models of Noonan syndrome causing PTPN11 gain-of-function mutations Hum. Mol. Genet., February 15, 2006; 15(4): 543 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Werz, T. V. Lee, P. L. Lee, M. Lackey, C. Bolduc, D. S. Stein, and A. Bergmann Mis-specified cells die by an active gene-directed process, and inhibition of this death results in cell fate transformation in Drosophila Development, December 15, 2005; 132(24): 5343 - 5352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Hellman, A. J. Greco, K. K. Rogers, C. Kanchagar, D. F. Balkovetz, and J. H. Lipschutz Activated extracellular signal-regulated kinases are necessary and sufficient to initiate tubulogenesis in renal tubular MDCK strain I cell cysts Am J Physiol Renal Physiol, October 1, 2005; 289(4): F777 - F785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ebisuya, K. Kondoh, and E. Nishida The duration, magnitude and compartmentalization of ERK MAP kinase activity: mechanisms for providing signaling specificity J. Cell Sci., July 15, 2005; 118(14): 2997 - 3002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Zhu, R. Wilson, and M. Leptin A Screen for Genes That Influence Fibroblast Growth Factor Signal Transduction in Drosophila Genetics, June 1, 2005; 170(2): 767 - 777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sano, H. Akimaru, T. Okamura, T. Nagao, M. Okada, and S. Ishii Drosophila Activating Transcription Factor-2 Is Involved in Stress Response via Activation by p38, but Not c-Jun NH2-Terminal Kinase Mol. Biol. Cell, June 1, 2005; 16(6): 2934 - 2946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wilson, E. Vogelsang, and M. Leptin FGF signalling and the mechanism of mesoderm spreading in Drosophila embryos Development, February 1, 2005; 132(3): 491 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kato, T. Chihara, and S. Hayashi Hedgehog and Decapentaplegic instruct polarized growth of cell extensions in the Drosophila trachea Development, November 1, 2004; 131(21): 5253 - 5261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schumacher, T. Gryzik, S. Tannebaum, and H.-A. J. Muller The RhoGEF Pebble is required for cell shape changes during cell migration triggered by the Drosophila FGF receptor Heartless Development, June 1, 2004; 131(11): 2631 - 2640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Smallhorn, M. J. Murray, and R. Saint The epithelial-mesenchymal transition of the Drosophila mesoderm requires the Rho GTP exchange factor Pebble Development, June 1, 2004; 131(11): 2641 - 2651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Petit, U. Nussbaumer, C. Dossenbach, and M. Affolter Downstream-of-FGFR Is a Fibroblast Growth Factor-Specific Scaffolding Protein and Recruits Corkscrew upon Receptor Activation Mol. Cell. Biol., May 1, 2004; 24(9): 3769 - 3781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Stathopoulos, B. Tam, M. Ronshaugen, M. Frasch, and M. Levine pyramus and thisbe: FGF genes that pattern the mesoderm of Drosophila embryos Genes & Dev., March 15, 2004; 18(6): 687 - 699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Deshpande, G. Calhoun, and P. Schedl Overlapping mechanisms function to establish transcriptional quiescence in the embryonic Drosophila germline Development, March 15, 2004; 131(6): 1247 - 1257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Stute, K. Schimmelpfeng, R. Renkawitz-Pohl, R. H. Palmer, and A. Holz Myoblast determination in the somatic and visceral mesoderm depends on Notch signalling as well as on milliways(miliAlk) as receptor for Jeb signalling Development, February 15, 2004; 131(4): 743 - 754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Dammai, B. Adryan, K. R. Lavenburg, and T. Hsu Drosophila awd, the homolog of human nm23, regulates FGF receptor levels and functions synergistically with shi/dynamin during tracheal development Genes & Dev., November 15, 2003; 17(22): 2812 - 2824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Corson, Y. Yamanaka, K.-M. V. Lai, and J. Rossant Spatial and temporal patterns of ERK signaling during mouse embryogenesis Development, October 1, 2003; 130(19): 4527 - 4537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ghiglione, L. Amundadottir, M. Andresdottir, D. Bilder, J. A. Diamonti, S. Noselli, N. Perrimon, and K. L. Carraway III Mechanism of inhibition of the Drosophila and mammalian EGF receptors by the transmembrane protein Kekkon 1 Development, September 15, 2003; 130(18): 4483 - 4493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Sepp and V. J. Auld Reciprocal Interactions between Neurons and Glia Are Required for Drosophila Peripheral Nervous System Development J. Neurosci., September 10, 2003; 23(23): 8221 - 8230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Urbach, R. Schnabel, and G. M. Technau The pattern of neuroblast formation, mitotic domains and proneural gene expression during early brain development in Drosophila Development, August 15, 2003; 130(16): 3589 - 3606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Hoeffer, S. Sanyal, and M. Ramaswami Acute Induction of Conserved Synaptic Signaling Pathways in Drosophila Melanogaster J. Neurosci., July 16, 2003; 23(15): 6362 - 6372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Chihara, K. Kato, M. Taniguchi, J. Ng, and S. Hayashi Rac promotes epithelial cell rearrangement during tracheal tubulogenesis in Drosophila Development, April 1, 2003; 130(7): 1419 - 1428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. H. Alsan and T. M. Schultheiss Regulation of avian cardiogenesis by Fgf8 signaling Development, March 6, 2003; 129(8): 1935 - 1943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Dumstrei, F. Wang, D. Shy, U. Tepass, and V. Hartenstein Interaction between EGFR signaling and DE-cadherin during nervous system morphogenesis Development, September 1, 2002; 129(17): 3983 - 3994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Koh, C. Ruiz-Canada, M. Gorczyca, and V. Budnik The Ras1-Mitogen-Activated Protein Kinase Signal Transduction Pathway Regulates Synaptic Plasticity through Fasciclin II-Mediated Cell Adhesion J. Neurosci., April 1, 2002; 22(7): 2496 - 2504. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Guichard, S. Srinivasan, G. Zimm, and E. Bier A screen for dominant mutations applied to components in the Drosophila EGF-R pathway PNAS, March 19, 2002; 99(6): 3752 - 3757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Kling, H. K. Lorenzo, A. M. Trbovich, T. B. Kinane, P. K. Donahoe, and J. J. Schnitzer Pre- and Postnatal Lung Development, Maturation, and Plasticity: MEK-1/2 inhibition reduces branching morphogenesis and causes mesenchymal cell apoptosis in fetal rat lungs Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L370 - L378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ghiglione, E. A. Bach, Y. Paraiso, K. L. Carraway III, S. Noselli, and N. Perrimon Mechanism of activation of the Drosophila EGF Receptor by the TGF{alpha} ligand Gurken during oogenesis Development, January 1, 2002; 129(1): 175 - 186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shinya, S. Koshida, A. Sawada, A. Kuroiwa, and H. Takeda Fgf signalling through MAPK cascade is required for development of the subpallial telencephalon in zebrafish embryos Development, November 1, 2001; 128(21): 4153 - 4164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Fisher, L. Michael, M. W. Barnett, and J. A. Davies Erk MAP kinase regulates branching morphogenesis in the developing mouse kidney Development, November 1, 2001; 128(21): 4329 - 4338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Janody, R. Sturny, V. Schaeffer, Y. Azou, and N. Dostatni Two distinct domains of Bicoid mediate its transcriptional downregulation by the Torso pathway Development, June 15, 2001; 128(12): 2281 - 2290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ciapponi, D. B. Jackson, M. Mlodzik, and D. Bohmann Drosophila Fos mediates ERK and JNK signals via distinct phosphorylation sites Genes & Dev., June 15, 2001; 15(12): 1540 - 1553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Glazer and B. Shilo Hedgehog signaling patterns the tracheal branches Development, January 5, 2001; 128(9): 1599 - 1606. [Abstract] [PDF]
|
|
|
|
|
|
J. Lorenzen, S. Baker, F Denhez, M. Melnick, D. Brower, and L. Perkins Nuclear import of activated D-ERK by DIM-7, an importin family member encoded by the gene moleskin Development, January 4, 2001; 128(8): 1403 - 1414. [Abstract] [PDF]
|
|
|
|
|
|
B San Martin and M Bate Hindgut visceral mesoderm requires an ectodermal template for normal development in Drosophila Development, January 1, 2001; 128(2): 233 - 242. [Abstract] [PDF]
|
|
|
|
|
|
G. Jiménez, A. Guichet, A. Ephrussi, and J. Casanova Relief of gene repression by Torso RTK signaling: role of capicua in Drosophila terminal and dorsoventral patterning Genes & Dev., January 15, 2000; 14(2): 224 - 231. [Abstract] [Full Text]
|
|
|
|
|
|
K Kubota, S Goto, K Eto, and S Hayashi EGF receptor attenuates Dpp signaling and helps to distinguish the wing and leg cell fates in Drosophila Development, January 9, 2000; 127(17): 3769 - 3776. [Abstract] [PDF]
|
|
|
|
|
|
F Janody, R Sturny, F Catala, C Desplan, and N Dostatni Phosphorylation of bicoid on MAP-kinase sites: contribution to its interaction with the torso pathway Development, January 1, 2000; 127(2): 279 - 289. [Abstract] [PDF]
|
|
|
|
|
|
F. Imam, D. Sutherland, W. Huang, and M. A. Krasnow stumps, a Drosophila Gene Required for Fibroblast Growth Factor (FGF)-directed Migrations of Tracheal and Mesodermal Cells Genetics, May 1, 1999; 152(1): 307 - 318. [Abstract] [Full Text]
|
|
|
|
|
|
T. Adachi-Yamada, M. Nakamura, K. Irie, Y. Tomoyasu, Y. Sano, E. Mori, S. Goto, N. Ueno, Y. Nishida, and K. Matsumoto p38 Mitogen-Activated Protein Kinase Can Be Involved in Transforming Growth Factor beta Superfamily Signal Transduction in Drosophila Wing Morphogenesis Mol. Cell. Biol., March 1, 1999; 19(3): 2322 - 2329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Ikeya and S Hayashi Interplay of Notch and FGF signaling restricts cell fate and MAPK activation in the Drosophila trachea Development, January 10, 1999; 126(20): 4455 - 4463. [Abstract] [PDF]
|
|
|
|
|
|
B Condron Spatially discrete FGF-mediated signalling directs glial morphogenesis Development, January 10, 1999; 126(20): 4635 - 4641. [Abstract] [PDF]
|
|
|
|
|
|
X Lin, E. Buff, N Perrimon, and A. Michelson Heparan sulfate proteoglycans are essential for FGF receptor signaling during Drosophila embryonic development Development, January 9, 1999; 126(17): 3715 - 3723. [Abstract] [PDF]
|
|
|
|
|
|
A Reich, A Sapir, and B Shilo Sprouty is a general inhibitor of receptor tyrosine kinase signaling Development, January 9, 1999; 126(18): 4139 - 4147. [Abstract] [PDF]
|
|
|
|
|
|
A Daniel, K Dumstrei, J. Lengyel, and V Hartenstein The control of cell fate in the embryonic visual system by atonal, tailless and EGFR signaling Development, January 7, 1999; 126(13): 2945 - 2954. [Abstract] [PDF]
|
|
|
|
|
|
M Llimargas The Notch pathway helps to pattern the tips of the Drosophila tracheal branches by selecting cell fates Development, January 6, 1999; 126(11): 2355 - 2364. [Abstract] [PDF]
|
|
|
|
|
|
A Guichard, B Biehs, M. Sturtevant, L Wickline, J Chacko, K Howard, and E Bier rhomboid and Star interact synergistically to promote EGFR/MAPK signaling during Drosophila wing vein development Development, January 6, 1999; 126(12): 2663 - 2676. [Abstract] [PDF]
|
|
|
|
|
|
B Christen and J. Slack Spatial response to fibroblast growth factor signalling in Xenopus embryos Development, January 1, 1999; 126(1): 119 - 125. [Abstract] [PDF]
|
|
|
|
|
|
A. Carmena, S. Gisselbrecht, J. Harrison, F. Jiménez, and A. M. Michelson Combinatorial signaling codes for the progressive determination of cell fates in the Drosophila embryonic mesoderm Genes & Dev., December 15, 1998; 12(24): 3910 - 3922. [Abstract] [Full Text]
|
|
|
|
|
|
S. Spencer, P. Powell, D. Miller, and R. Cagan Regulation of EGF receptor signaling establishes pattern across the developing Drosophila retina Development, January 12, 1998; 125(23): 4777 - 4790. [Abstract] [PDF]
|
|
|
|
|
|
A. Michelson, S Gisselbrecht, E Buff, and J. Skeath Heartbroken is a specific downstream mediator of FGF receptor signalling in Drosophila Development, January 11, 1998; 125(22): 4379 - 4389. [Abstract] [PDF]
|
|
|
|
|
|
J. Kumar, M Tio, F Hsiung, S Akopyan, L Gabay, R Seger, B. Shilo, and K Moses Dissecting the roles of the Drosophila EGF receptor in eye development and MAP kinase activation Development, January 10, 1998; 125(19): 3875 - 3885. [Abstract] [PDF]
|
|
