|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 124, Issue 19 3909-3917, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
PT Martin and JR Sanes
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, MO 63110, USA.
Agrin, a basal lamina-associated proteoglycan, is a crucial nerve-derived organizer of postsynaptic differentiation at the skeletal neuromuscular junction. Because integrins serve as cellular receptors for many basal lamina components, we asked whether agrin interacts with integrins. Agrin-induced aggregation of acetylcholine receptors on cultured myotubes was completely blocked by antibodies to the beta1 integrin subunit and partially blocked by antibodies to the alpha(v) integrin subunit. Agrin-induced clustering was also inhibited by antisense oligonucleotides to alpha(v) and a peptide that blocks the alpha(v) binding site. Non-muscle cells that expressed alpha(v) and beta1 integrin subunits adhered to immobilized agrin, and this adhesion was blocked by anti-alpha(v) and anti-beta1 antibodies. Integrin alpha(v)-negative cells that did not adhere to agrin were rendered adherent by introduction of alpha(v). Together, these results implicate integrins, including alpha(v)beta1, as components or modulators of agrin's signal transduction pathway.
This article has been cited by other articles:
|
|
 |
|
  S. J. Harvey, G. Jarad, J. Cunningham, A. L. Rops, J. van der Vlag, J. H. Berden, M. J. Moeller, L. B. Holzman, R. W. Burgess, and J. H. Miner Disruption of Glomerular Basement Membrane Charge through Podocyte-Specific Mutation of Agrin Does Not Alter Glomerular Permselectivity Am. J. Pathol., July 1, 2007; 171(1): 139 - 152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Huang, K. Shimazu, N. H. Woo, K. Zang, U. Muller, B. Lu, and L. F. Reichardt Distinct Roles of the beta1-Class Integrins at the Developing and the Mature Hippocampal Excitatory Synapse J. Neurosci., October 25, 2006; 26(43): 11208 - 11219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Alfsen, H. Yu, A. Magerus-Chatinet, A. Schmitt, and M. Bomsel HIV-1-infected Blood Mononuclear Cells Form an Integrin- and Agrin-dependent Viral Synapse to Induce Efficient HIV-1 Transcytosis across Epithelial Cell Monolayer Mol. Biol. Cell, September 1, 2005; 16(9): 4267 - 4279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schwander, R. Shirasaki, S. L. Pfaff, and U. Muller {beta}1 Integrins in Muscle, But Not in Motor Neurons, Are Required for Skeletal Muscle Innervation J. Neurosci., September 15, 2004; 24(37): 8181 - 8191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Shiao, A. Fond, B. Deng, M. Wehling-Henricks, M. E. Adams, S. C. Froehner, and J. G. Tidball Defects in neuromuscular junction structure in dystrophic muscle are corrected by expression of a NOS transgene in dystrophin-deficient muscles, but not in muscles lacking {alpha}- and {beta}1-syntrophins Hum. Mol. Genet., September 1, 2004; 13(17): 1873 - 1884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. T. Martin Dystroglycan glycosylation and its role in matrix binding in skeletal muscle Glycobiology, August 1, 2003; 13(8): 55R - 66R. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gingras, S. Rassadi, E. Cooper, and M. Ferns Agrin plays an organizing role in the formation of sympathetic synapses J. Cell Biol., September 16, 2002; 158(6): 1109 - 1118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Marangi, S. T. Wieland, and C. Fuhrer Laminin-1 redistributes postsynaptic proteins and requires rapsyn, tyrosine phosphorylation, and Src and Fyn to stably cluster acetylcholine receptors J. Cell Biol., May 28, 2002; 157(5): 883 - 895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-S. Uhm, B. Neuhuber, B. Lowe, V. Crocker, and M. P. Daniels Synapse-Forming Axons and Recombinant Agrin Induce Microprocess Formation on Myotubes J. Neurosci., December 15, 2001; 21(24): 9678 - 9689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. P. Moiseeva Adhesion receptors of vascular smooth muscle cells and their functions Cardiovasc Res, December 1, 2001; 52(3): 372 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jacobson, P. D. Cote, S. G. Rossi, R. L. Rotundo, and S. Carbonetto The Dystroglycan Complex Is Necessary for Stabilization of Acetylcholine Receptor Clusters at Neuromuscular Junctions and Formation of the Synaptic Basement Membrane J. Cell Biol., January 29, 2001; 152(3): 435 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Bose, D. Qiu, A. Bergamaschi, B. Gravante, M. Bossi, A. Villa, F. Rupp, and A. Malgaroli Agrin Controls Synaptic Differentiation in Hippocampal Neurons J. Neurosci., December 15, 2000; 20(24): 9086 - 9095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Erickson and J. R. Couchman Still More Complexity in Mammalian Basement Membranes J. Histochem. Cytochem., October 1, 2000; 48(10): 1291 - 1306. [Abstract] [Full Text]
|
|
|
|
|
|
R. W. Burgess, W. C. Skarnes, and J. R. Sanes Agrin Isoforms with Distinct Amino Termini: Differential Expression, Localization, and Function J. Cell Biol., September 25, 2000; 151(1): 41 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Cohen, B. G. Hoffstrom, and D. W. DeSimone Active Zones on Motor Nerve Terminals Contain alpha 3beta 1 Integrin J. Neurosci., July 1, 2000; 20(13): 4912 - 4921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. I. Raats, J. van den Born, M. A. H. Bakker, B. Oppers-Walgreen, B. J. M. Pisa, H. B. P. M. Dijkman, K. J. M. Assmann, and J. H. M. Berden Expression of Agrin, Dystroglycan, and Utrophin in Normal Renal Tissue and in Experimental Glomerulopathies Am. J. Pathol., May 1, 2000; 156(5): 1749 - 1765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Burkin, J. Kim, M Gu, and S. Kaufman Laminin and alpha7beta1 integrin regulate agrin-induced clustering of acetylcholine receptors J. Cell Sci., January 8, 2000; 113(16): 2877 - 2886. [Abstract] [PDF]
|
|
|
|
 |
|
 A. J. A. Groffen, J. H. Veerkamp, L. A. H. Monnens, and L. P. W. J. van den Heuvel Recent insights into the structure and functions of heparan sulfate proteoglycans in the human glomerular basement membrane Nephrol. Dial. Transplant., September 1, 1999; 14(9): 2119 - 2129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Jones, C. Moore, S. Hashemolhosseini, and H. R. Brenner Constitutively Active MuSK Is Clustered in the Absence of Agrin and Induces Ectopic Postsynaptic-Like Membranes in Skeletal Muscle Fibers J. Neurosci., May 1, 1999; 19(9): 3376 - 3383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Cornish, J Chi, S Johnson, Y Lu, and J. Campanelli Globular domains of agrin are functional units that collaborate to induce acetylcholine receptor clustering J. Cell Sci., January 4, 1999; 112(8): 1213 - 1223. [Abstract] [PDF]
|
|
|
|
|
|
D. J. Burkin, M. Gu, B. L. Hodges, J. T. Campanelli, and S. J. Kaufman A Functional Role for Specific Spliced Variants of the alpha 7beta 1 Integrin in Acetylcholine Receptor Clustering J. Cell Biol., November 16, 1998; 143(4): 1067 - 1075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. De Deyne, A O'Neill, W. Resneck, G. Dmytrenko, D. Pumplin, and R. Bloch The vitronectin receptor associates with clathrin-coated membrane domains via the cytoplasmic domain of its beta5 subunit J. Cell Sci., January 9, 1998; 111(18): 2729 - 2740. [Abstract] [PDF]
|
|
|
|
 |
|
 P. Mittaud, P. A. Marangi, S. Erb-Vogtli, and C. Fuhrer Agrin-induced Activation of Acetylcholine Receptor-bound Src Family Kinases Requires Rapsyn and Correlates with Acetylcholine Receptor Clustering J. Biol. Chem., April 20, 2001; 276(17): 14505 - 14513. [Abstract] [Full Text] [PDF]
|
|
