|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 124, Issue 21 4361-4371, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
P Gonczy, E Matunis and S DiNardo
The Rockefeller University, New York City, New York 10021-6399, USA.
Stem cells divide asymmetrically, regenerating a parental stem cell and giving rise to a daughter cell with a distinct fate. In many stem cell lineages, this daughter cell undergoes several amplificatory mitoses, thus generating more cells that embark on the differentiation program specific for the given lineage. Spermatogenesis in Drosophila is a model system to identify molecules regulating stem cell lineages. Mutations at two previously identified loci, bag-of-marbles (bam) and benign gonial cell neoplasm (bgcn), prevent progression through spermatogenesis and oogenesis, resulting in the overproliferation of undifferentiated germ cells. Here we investigate how bam and bgcn regulate the male germline stem cell lineage. By generating FLP-mediated clones, we demonstrate that both bam and bgcn act autonomously in the germline to restrict proliferation during spermatogenesis. By using enhancer trap lines, we find that the overproliferating germ cells express markers specific to amplifying germ cells, while at the same time retaining the expression of some markers of stem cell and primary spermatogonial cell fate. However, we find that germ cells accumulating in bam or bgcn mutant testes most resemble amplifying germ cells, because they undergo incomplete cytokinesis and progress through the cell cycle in synchrony within a cyst, which are two characteristics of amplifying germ cells, but not of stem cells. Taken together, our results suggest that bam and bgcn regulate progression through the male germline stem cell lineage by cell-intrinsically restricting the proliferation of amplifying germ cells.
This article has been cited by other articles:
|
|
 |
|
  V. L. Bauer DuMont, H. A. Flores, M. H. Wright, and C. F. Aquadro Recurrent Positive Selection at Bgcn, a Key Determinant of Germ Line Differentiation, Does Not Appear to be Driven by Simple Coevolution with Its Partner Protein Bam Mol. Biol. Evol., January 1, 2007; 24(1): 182 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Mikhaylova, A. M. Boutanaev, and D. I. Nurminsky Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line PNAS, August 8, 2006; 103(32): 11975 - 11980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Casper and M. Van Doren The control of sexual identity in the Drosophila germline Development, August 1, 2006; 133(15): 2783 - 2791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Jin, E. M. Homola, P. Goldbach, Y. Choi, J. A. Brill, and S. D. Campbell Drosophila Myt1 is a Cdk1 inhibitory kinase that regulates multiple aspects of cell cycle behavior during gametogenesis Development, September 15, 2005; 132(18): 4075 - 4085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gilboa and R. Lehmann How different is Venus from Mars? The genetics of germ-line stem cells in Drosophila females and males Development, October 15, 2004; 131(20): 4895 - 4905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Baker, S. Adhikarakunnathu, and M. J. Kernan Mechanosensory-defective, male-sterile unc mutants identify a novel basal body protein required for ciliogenesis in Drosophila Development, July 15, 2004; 131(14): 3411 - 3422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Schulz, A. A. Kiger, S. I. Tazuke, Y. M. Yamashita, L. C. Pantalena-Filho, D. L. Jones, C. G. Wood, and M. T. Fuller A Misexpression Screen Reveals Effects of bag-of-marbles and TGF{beta} Class Signaling on the Drosophila Male Germ-Line Stem Cell Lineage Genetics, June 1, 2004; 167(2): 707 - 723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kawase, M. D. Wong, B. C. Ding, and T. Xie Gbb/Bmp signaling is essential for maintaining germline stem cells and for repressing bam transcription in the Drosophila testis Development, March 15, 2004; 131(6): 1365 - 1375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gilboa, A. Forbes, S. I. Tazuke, M. T. Fuller, and R. Lehmann Germ line stem cell differentiation in Drosophila requires gap junctions and proceeds via an intermediate state Development, December 29, 2003; 130(26): 6625 - 6634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Mason, R. J. Fleming, and D. S. Goldfarb Drosophila melanogaster Importin {alpha}1 and {alpha}3 Can Replace Importin {alpha}2 During Spermatogenesis but Not Oogenesis Genetics, May 1, 2002; 161(1): 157 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Schulz, C. G. Wood, D. L. Jones, S. I. Tazuke, and M. T. Fuller Signaling from germ cells mediated by the rhomboid homolog stet organizes encapsulation by somatic support cells Development, January 10, 2002; 129(19): 4523 - 4534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Page, K. S. McKim, B. Deneen, T. L. Van Hook, and R. S. Hawley Genetic Studies of mei-P26 Reveal a Link Between the Processes That Control Germ Cell Proliferation in Both Sexes and Those That Control Meiotic Exchange in Drosophila Genetics, August 1, 2000; 155(4): 1757 - 1772. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Kim-Ha, J. Kim, and Y.-J. Kim Requirement of RBP9, a Drosophila Hu Homolog, for Regulation of Cystocyte Differentiation and Oocyte Determination during Oogenesis Mol. Cell. Biol., April 1, 1999; 19(4): 2505 - 2514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kadyk and J Kimble Genetic regulation of entry into meiosis in Caenorhabditis elegans Development, January 5, 1998; 125(10): 1803 - 1813. [Abstract] [PDF]
|
|
