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Development, Vol 124, Issue 5 1019-1031, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
TL Davis and BJ Meyer
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA.
X chromosome expression in C. elegans is controlled by a chromosome-wide regulatory process called dosage compensation that specifically reduces by half the level of transcripts made from each hermaphrodite X chromosome. This process equalizes X expression between the sexes (XX hermaphrodites and XO males), despite their two-fold difference in X chromosome dose, and thereby prevents sex-specific lethality. Dosage compensation is achieved by a protein complex that associates with X in a sex-specific fashion to modulate gene expression. SDC-3, a protein that coordinately controls both sex determination and dosage compensation, activates dosage compensation by directing the dosage compensation protein complex to the hermaphrodite X chromosomes. We show that SDC-3 coordinates this assembly through its own sex-specific association with X. SDC-3 in turn requires other members of the dosage compensation gene hierarchy for its stability and its X localization. In addition, SDC-3 requires its own zinc finger motifs and an amino-terminal region for its X association. Our experiments suggest the possible involvement of zinc finger motifs in X chromosome recognition and the amino-terminal region in interactions with other dosage compensation proteins.
This article has been cited by other articles:
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  J. M. Gladden and B. J. Meyer A ONECUT Homeodomain Protein Communicates X Chromosome Dose to Specify Caenorhabditis elegans Sexual Fate by Repressing a Sex Switch Gene Genetics, November 1, 2007; 177(3): 1621 - 1637. [Abstract] [Full Text] [PDF]
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J. M. Gladden, B. Farboud, and B. J. Meyer Revisiting the X:A Signal That Specifies Caenorhabditis elegans Sexual Fate Genetics, November 1, 2007; 177(3): 1639 - 1654. [Abstract] [Full Text] [PDF]
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D. S. Chu, H. E. Dawes, J. D. Lieb, R. C. Chan, A. F. Kuo, and B. J. Meyer A molecular link between gene-specific and chromosome-wide transcriptional repression Genes & Dev., April 1, 2002; 16(7): 796 - 805. [Abstract] [Full Text] [PDF]
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 P. L. Nagy, J. Griesenbeck, R. D. Kornberg, and M. L. Cleary A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3 PNAS, December 13, 2001; (2001) 221596698. [Abstract] [Full Text] [PDF]
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J. D. Lieb, C. O. de Solorzano, E. G. Rodriguez, A. Jones, M. Angelo, S. Lockett, and B. J. Meyer The Caenorhabditis elegans Dosage Compensation Machinery Is Recruited to X Chromosome DNA Attached to an Autosome Genetics, December 1, 2000; 156(4): 1603 - 1621. [Abstract] [Full Text]
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I. Carmi and B. J. Meyer The Primary Sex Determination Signal of Caenorhabditis elegans Genetics, July 1, 1999; 152(3): 999 - 1015. [Abstract] [Full Text]
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W. Li, A. Streit, B. Robertson, and W. B. Wood Evidence for Multiple Promoter Elements Orchestrating Male-Specific Regulation of the her-1 Gene in Caenorhabditis elegans Genetics, May 1, 1999; 152(1): 237 - 248. [Abstract] [Full Text]
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R Holdeman, S Nehrt, and S Strome MES-2, a maternal protein essential for viability of the germline in Caenorhabditis elegans, is homologous to a Drosophila Polycomb group protein Development, January 7, 1998; 125(13): 2457 - 2467. [Abstract] [PDF]
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The Phenotype of mes-2, mes-3, mes-4 and mes-6, Maternal-Effect Genes Required for Survival of the Germline in Caenorhabditis elegans, Is Sensitive to Chromosome Dosage Genetics, January 1, 1998; 148(1): 167 - 186.
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P. L. Nagy, J. Griesenbeck, R. D. Kornberg, and M. L. Cleary A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3 PNAS, January 8, 2002; 99(1): 90 - 94. [Abstract] [Full Text] [PDF]
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