|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 125, Issue 17 3343-3352, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
RS Ginger, L Drury, C Baader, NV Zhukovskaya and JG Williams
MRC Laboratory of Molecular Cell Biology and Department of Biology, University College London, Gower Street, London WC1E 6BT, UK.
A mutant of Dictyostelium that is aberrant in the process of tip formation (dtfA-: defective in tip formation A) has been isolated by gene tagging. The dtfA gene is predicted to encode a protein of 163 kDa. There are no extensive sequence homologies between DTFA and previously identified proteins, but four short N-terminal sequence motifs show partial homology to repeats found in mammalian mucins. Immunofluorescence reveals a lattice-like arrangement of DTFA protein at the cell surface. When developing on a bacterial lawn, cells of the mutant strain (dtfA- cells) aggregate to form tight mounds, but development then becomes arrested. When developed in the absence of nutrients, a fraction of dtfA- cells complete development, but there is a long delay at the tight mound stage and the culminants that eventually form are aberrant. In such dtfA- mounds the prestalk cells fail to move to the apex on cue and so tip formation is delayed. dtfA- cells also show a conditional defect in early development, in that they are unable to aggregate when plated at low density. In addition dtfA- cells do not agglomerate efficiently when shaken in suspension. In combination, these results suggest that DTFA may form part of a cell-cell adhesion system that is needed both for optimal aggregation and for efficient cell sorting during multicellular development. The DTFA protein also appears to be important during cell growth, because cytokinesis is defective and the actin cytoskeleton aberrant in growing dtfA- cells.
This article has been cited by other articles:
|
|
 |
|
  E. Wong, C. Yang, J. Wang, D. Fuller, W. F. Loomis, and C.-H. Siu Disruption of the gene encoding the cell adhesion molecule DdCAD-1 leads to aberrant cell sorting and cell-type proportioning during Dictyostelium development Development, March 10, 2003; 129(16): 3839 - 3850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Friedl, S. Borgmann, and E.-B. Brocker Amoeboid leukocyte crawling through extracellular matrix: lessons from the Dictyostelium paradigm of cell movement J. Leukoc. Biol., October 1, 2001; 70(4): 491 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yoshida and K. Inouye Myosin II-dependent cylindrical protrusions induced by quinine in Dictyostelium: antagonizing effects of actin polymerization at the leading edge J. Cell Sci., January 6, 2001; 114(11): 2155 - 2165. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chien, C. Y. Chung, S. Sukumaran, N. Osborne, S. Lee, C. Ellsworth, J. G. McNally, and R. A. Firtel The Dictyostelium LIM Domain-containing Protein LIM2 Is Essential for Proper Chemotaxis and Morphogenesis Mol. Biol. Cell, April 1, 2000; 11(4): 1275 - 1291. [Abstract] [Full Text]
|
|
