Development, Vol 125, Issue 19 3789-3799, Copyright © 1998 by Company of Biologists

JOURNAL ARTICLES

The Arabidopsis thaliana gametophytic mutation gemini pollen1 disrupts microspore polarity, division asymmetry and pollen cell fate

SK Park, R Howden and D Twell
Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK.

Pollen development and male gametogenesis are critically dependent upon cell polarization leading to a highly asymmetric cell division termed pollen mitosis I. A mutational approach was adopted in Arabidopsis thaliana to identify genes involved these processes. Four independent gemini pollen mutants were isolated which produce divided or twin-celled pollen. The gemini pollen1 mutant was characterized in detail and shown to act gametophytically resulting in reduced transmission through both sexes. gemini pollen1 showed an incompletely penetrant phenotype resulting in equal, unequal and partial divisions at pollen mitosis I. The division planes in gemini pollen1 were shown to be aligned with the polar axis (as in wild type) and evidence was obtained for incomplete nuclear migration, which could account for altered division symmetry. gemini pollen1 also showed division phenotypes consistent with spatial uncoupling of karyokinesis and cytokinesis suggesting that GEMINI POLLEN1 may be required for the localization of phragmoplast activity. Cell fate studies showed that in both equal and unequal divisions a vegetative cell marker gene was activated in both daughter cells. Daughter cells with a range of intermediate or hybrid vegetative/generative cell fates suggests that cell fate is quantitatively related to cell size. The potential mode of action of GEMINI POLLEN1 and its effects on cell fate are discussed in relation to proposed models of microspore polarity and cell fate determination.

This article has been cited by other articles:

				S.-A. Oh, V. Bourdon, M. Das 'Pal, H. Dickinson, and D. Twell Arabidopsis Kinesins HINKEL and TETRASPORE Act Redundantly to Control Cell Plate Expansion during Cytokinesis in the Male Gametophyte Mol Plant, September 1, 2008; 1(5): 794 - 799. [Abstract] [Full Text] [PDF]

				Y. Lee, E.-S. Kim, Y. Choi, I. Hwang, C. J. Staiger, Y.-Y. Chung, and Y. Lee The Arabidopsis Phosphatidylinositol 3-Kinase Is Important for Pollen Development Plant Physiology, August 1, 2008; 147(4): 1886 - 1897. [Abstract] [Full Text] [PDF]

				J. Liu and L.-J. Qu Meiotic and Mitotic Cell Cycle Mutants Involved in Gametophyte Development in Arabidopsis Mol Plant, July 1, 2008; 1(4): 564 - 574. [Abstract] [Full Text] [PDF]

				J. Liu, Y. Zhang, G. Qin, T. Tsuge, N. Sakaguchi, G. Luo, K. Sun, D. Shi, S. Aki, N. Zheng, et al. Targeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis Gametogenesis PLANT CELL, June 1, 2008; 20(6): 1538 - 1554. [Abstract] [Full Text] [PDF]

				C. Teng, H. Dong, L. Shi, Y. Deng, J. Mu, J. Zhang, X. Yang, and J. Zuo Serine Palmitoyltransferase, a Key Enzyme for de Novo Synthesis of Sphingolipids, Is Essential for Male Gametophyte Development in Arabidopsis Plant Physiology, March 1, 2008; 146(3): 1322 - 1332. [Abstract] [Full Text] [PDF]

				Y.-R. J. Lee, Y. Li, and B. Liu Two Arabidopsis Phragmoplast-Associated Kinesins Play a Critical Role in Cytokinesis during Male Gametogenesis PLANT CELL, August 1, 2007; 19(8): 2595 - 2605. [Abstract] [Full Text] [PDF]

				S. Footitt, D. Dietrich, A. Fait, A. R. Fernie, M. J. Holdsworth, A. Baker, and F. L. Theodoulou The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis Plant Physiology, July 1, 2007; 144(3): 1467 - 1480. [Abstract] [Full Text] [PDF]

				G. Leon, L. Holuigue, and X. Jordana Mitochondrial Complex II Is Essential for Gametophyte Development in Arabidopsis Plant Physiology, April 1, 2007; 143(4): 1534 - 1546. [Abstract] [Full Text] [PDF]

				K. von Besser, A. C. Frank, M. A. Johnson, and D. Preuss Arabidopsis HAP2 (GCS1) is a sperm-specific gene required for pollen tube guidance and fertilization Development, December 1, 2006; 133(23): 4761 - 4769. [Abstract] [Full Text] [PDF]

				G. Drakakaki, O. Zabotina, I. Delgado, S. Robert, K. Keegstra, and N. Raikhel Arabidopsis Reversibly Glycosylated Polypeptides 1 and 2 Are Essential for Pollen Development Plant Physiology, December 1, 2006; 142(4): 1480 - 1492. [Abstract] [Full Text] [PDF]

				M.-J. Han, K.-H. Jung, G. Yi, D.-Y. Lee, and G. An Rice Immature Pollen 1 (RIP1) is a Regulator of Late Pollen Development Plant Cell Physiol., November 1, 2006; 47(11): 1457 - 1472. [Abstract] [Full Text] [PDF]

				M. Pastuglia, J. Azimzadeh, M. Goussot, C. Camilleri, K. Belcram, J.-L. Evrard, A.-C. Schmit, P. Guerche, and D. Bouchez {gamma}-Tubulin Is Essential for Microtubule Organization and Development in Arabidopsis PLANT CELL, June 1, 2006; 18(6): 1412 - 1425. [Abstract] [Full Text] [PDF]

				E. Kawamura, R. Himmelspach, M. C. Rashbrooke, A. T. Whittington, K. R. Gale, D. A. Collings, and G. O. Wasteneys MICROTUBULE ORGANIZATION 1 Regulates Structure and Function of Microtubule Arrays during Mitosis and Cytokinesis in the Arabidopsis Root Plant Physiology, January 1, 2006; 140(1): 102 - 114. [Abstract] [Full Text] [PDF]

				M. K. Jakobsen,, L. R. Poulsen,, A. Schulz,, P. Fleurat-Lessard,, A. Moller,, S. Husted,, M. Schiott,, A. Amtmann,, and M. G. Palmgren, Pollen development and fertilization in Arabidopsis is dependent on the MALE GAMETOGENESIS IMPAIRED ANTHERS gene encoding a Type V P-type ATPase Genes & Dev., November 15, 2005; 19(22): 2757 - 2769. [Abstract] [Full Text] [PDF]

				R. A. Cole, L. Synek, V. Zarsky, and J. E. Fowler SEC8, a Subunit of the Putative Arabidopsis Exocyst Complex, Facilitates Pollen Germination and Competitive Pollen Tube Growth Plant Physiology, August 1, 2005; 138(4): 2005 - 2018. [Abstract] [Full Text] [PDF]

				A. Baldwin, A. Wardle, R. Patel, P. Dudley, S. K. Park, D. Twell, K. Inoue, and P. Jarvis A Molecular-Genetic Study of the Arabidopsis Toc75 Gene Family Plant Physiology, June 1, 2005; 138(2): 715 - 733. [Abstract] [Full Text] [PDF]

				E. P. Eleftheriou, T. I. Baskin, and P. K. Hepler Aberrant Cell Plate Formation in the Arabidopsis thaliana microtubule organization 1 Mutant Plant Cell Physiol., April 1, 2005; 46(4): 671 - 675. [Abstract] [Full Text] [PDF]

				P. Niewiadomski, S. Knappe, S. Geimer, K. Fischer, B. Schulz, U. S. Unte, M. G. Rosso, P. Ache, U.-I. Flugge, and A. Schneider The Arabidopsis Plastidic Glucose 6-Phosphate/Phosphate Translocator GPT1 Is Essential for Pollen Maturation and Embryo Sac Development PLANT CELL, March 1, 2005; 17(3): 760 - 775. [Abstract] [Full Text] [PDF]

				A. Durbarry, I. Vizir, and D. Twell Male Germ Line Development in Arabidopsis. duo pollen Mutants Reveal Gametophytic Regulators of Generative Cell Cycle Progression Plant Physiology, January 1, 2005; 137(1): 297 - 307. [Abstract] [Full Text] [PDF]

				H. Tanaka, M. Ishikawa, S. Kitamura, Y. Takahashi, T. Soyano, C. Machida, and Y. Machida The AtNACK1/HINKEL and STUD/TETRASPORE/AtNACK2 genes, which encode functionally redundant kinesins, are essential for cytokinesis in Arabidopsis Genes Cells, December 1, 2004; 9(12): 1199 - 1211. [Abstract] [Full Text] [PDF]

				Z. A Wilson and C. Yang Plant gametogenesis: conservation and contrasts in development Reproduction, November 1, 2004; 128(5): 483 - 492. [Abstract] [Full Text] [PDF]

				M. A. Johnson, K. von Besser, Q. Zhou, E. Smith, G. Aux, D. Patton, J. Z. Levin, and D. Preuss Arabidopsis hapless Mutations Define Essential Gametophytic Functions Genetics, October 1, 2004; 168(2): 971 - 982. [Abstract] [Full Text] [PDF]

				H. Sze, S. Padmanaban, F. Cellier, D. Honys, N.-H. Cheng, K. W. Bock, G. Conejero, X. Li, D. Twell, J. M. Ward, et al. Expression Patterns of a Novel AtCHX Gene Family Highlight Potential Roles in Osmotic Adjustment and K+ Homeostasis in Pollen Development Plant Physiology, September 1, 2004; 136(1): 2532 - 2547. [Abstract] [Full Text] [PDF]

				H. Visscher, C. V. Looy, M. E. Collinson, H. Brinkhuis, J. H. A. van Konijnenburg-van Cittert, W. M. Kurschner, and M. A. Sephton From the Cover: Environmental mutagenesis during the end-Permian ecological crisis PNAS, August 31, 2004; 101(35): 12952 - 12956. [Abstract] [Full Text] [PDF]

				E. Lalanne, C. Michaelidis, J. M. Moore, W. Gagliano, A. Johnson, R. Patel, R. Howden, J.-P. Vielle-Calzada, U. Grossniklaus, and D. Twell Analysis of Transposon Insertion Mutants Highlights the Diversity of Mechanisms Underlying Male Progamic Development in Arabidopsis Genetics, August 1, 2004; 167(4): 1975 - 1986. [Abstract] [Full Text] [PDF]

				S. McCormick Control of Male Gametophyte Development PLANT CELL, June 1, 2004; 16(suppl_1): S142 - S153. [Full Text] [PDF]

				G. R. Hicks, E. Rojo, S. Hong, D. G. Carter, and N. V. Raikhel Geminating Pollen Has Tubular Vacuoles, Displays Highly Dynamic Vacuole Biogenesis, and Requires VACUOLESS1 for Proper Function Plant Physiology, March 1, 2004; 134(3): 1227 - 1239. [Abstract] [Full Text] [PDF]

				E. Lalanne, D. Honys, A. Johnson, G. H. H. Borner, K. S. Lilley, P. Dupree, U. Grossniklaus, and D. Twell SETH1 and SETH2, Two Components of the Glycosylphosphatidylinositol Anchor Biosynthetic Pathway, Are Required for Pollen Germination and Tube Growth in Arabidopsis PLANT CELL, January 1, 2004; 16(1): 229 - 240. [Abstract] [Full Text] [PDF]

				K. M. Arthur, Z. Vejlupkova, R. B. Meeley, and J. E. Fowler Maize ROP2 GTPase Provides a Competitive Advantage to the Male Gametophyte Genetics, December 1, 2003; 165(4): 2137 - 2151. [Abstract] [Full Text] [PDF]

				M. Golovkin and A. S. N. Reddy A calmodulin-binding protein from Arabidopsis has an essential role in pollen germination PNAS, September 2, 2003; 100(18): 10558 - 10563. [Abstract] [Full Text] [PDF]

				D. Honys and D. Twell Comparative Analysis of the Arabidopsis Pollen Transcriptome Plant Physiology, June 1, 2003; 132(2): 640 - 652. [Abstract] [Full Text] [PDF]

				F. Goubet, A. Misrahi, S. K. Park, Z. Zhang, D. Twell, and P. Dupree AtCSLA7, a Cellulose Synthase-Like Putative Glycosyltransferase, Is Important for Pollen Tube Growth and Embryogenesis in Arabidopsis Plant Physiology, February 1, 2003; 131(2): 547 - 557. [Abstract] [Full Text] [PDF]

				S. Muller, E. Fuchs, M. Ovecka, J. Wysocka-Diller, P. N. Benfey, and M.-T. Hauser Two New Loci, PLEIADE and HYADE, Implicate Organ-Specific Regulation of Cytokinesis in Arabidopsis Plant Physiology, September 1, 2002; 130(1): 312 - 324. [Abstract] [Full Text] [PDF]

				E. Lalanne and D. Twell Genetic Control of Male Germ Unit Organization in Arabidopsis Plant Physiology, June 1, 2002; 129(2): 865 - 875. [Abstract] [Full Text] [PDF]

				G. O. Wasteneys Microtubule organization in the green kingdom: chaos or self-order? J. Cell Sci., January 4, 2002; 115(7): 1345 - 1354. [Abstract] [Full Text] [PDF]

				N. A. Eckardt, T. Araki, C. Benning, P. Cubas, J. Goodrich, S. E. Jacobsen, P. Masson, E. Nambara, R. Simon, S. Somerville, et al. Arabidopsis Research 2001 PLANT CELL, September 1, 2001; 13(9): 1973 - 1982. [Full Text] [PDF]

				A. Procissi, S. de Laissardiere, M. Ferault, D. Vezon, G. Pelletier, and S. Bonhomme Five Gametophytic Mutations Affecting Pollen Development and Pollen Tube Growth in Arabidopsis thaliana Genetics, August 1, 2001; 158(4): 1773 - 1783. [Abstract] [Full Text] [PDF]

				S. A. Johnson and S. McCormick Pollen Germinates Precociously in the Anthers of raring-to-go, an Arabidopsis Gametophytic Mutant Plant Physiology, June 1, 2001; 126(2): 685 - 695. [Abstract] [Full Text] [PDF]

				S. K. Park and D. Twell Novel Patterns of Ectopic Cell Plate Growth and Lipid Body Distribution in the Arabidopsis gemini pollen1 Mutant Plant Physiology, June 1, 2001; 126(2): 899 - 909. [Abstract] [Full Text] [PDF]

				M. Geisler, J. Nadeau, and F. D. Sack Oriented Asymmetric Divisions That Generate the Stomatal Spacing Pattern in Arabidopsis Are Disrupted by the too many mouths Mutation PLANT CELL, November 1, 2000; 12(11): 2075 - 2086. [Abstract] [Full Text]

				G. P. Copenhaver, K. C. Keith, and D. Preuss Tetrad Analysis in Higher Plants. A Budding Technology Plant Physiology, September 1, 2000; 124(1): 7 - 16. [Abstract] [Full Text]