|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 125, Issue 20 3955-3966, Copyright © 1998 by Company of Biologists
JOURNAL ARTICLES |
O Papoulas, SJ Beek, SL Moseley, CM McCallum, M Sarte, A Shearn and JW Tamkun
Department of Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA. tamkun@biology.ucsc.edu.
The trithorax group gene brahma (brm) encodes an activator of Drosophila homeotic genes that functions as the ATPase subunit of a large protein complex. To determine if BRM physically interacts with other trithorax group proteins, we purified the BRM complex from Drosophila embryos and analyzed its subunit composition. The BRM complex contains at least seven major polypeptides. Surprisingly, the majority of the subunits of the BRM complex are not encoded by trithorax group genes. Furthermore, a screen for enhancers of a dominant-negative brm mutation identified only one trithorax group gene, moira (mor), that appears to be essential for brm function in vivo. Four of the subunits of the BRM complex are related to subunits of the yeast chromatin remodeling complexes SWI/SNF and RSC. The BRM complex is even more highly related to the human BRG1 and hBRM complexes, but lacks the subunit heterogeneity characteristic of these complexes. We present biochemical evidence for the existence of two additional complexes containing trithorax group proteins: a 2 MDa ASH1 complex and a 500 kDa ASH2 complex. These findings suggest that BRM plays a role in chromatin remodeling that is distinct from the function of most other trithorax group proteins.
This article has been cited by other articles:
|
|
 |
|
  S. Petruk, S. T. Smith, Y. Sedkov, and A. Mazo Association of trxG and PcG proteins with the bxd maintenance element depends on transcriptional activity Development, July 15, 2008; 135(14): 2383 - 2390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nakamura, H. Ida, and M. Yamaguchi Transcriptional regulation of the Drosophila moira and osa genes by the DREF pathway Nucleic Acids Res., July 1, 2008; 36(12): 3905 - 3915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Chalkley, Y. M. Moshkin, K. Langenberg, K. Bezstarosti, A. Blastyak, H. Gyurkovics, J. A. A. Demmers, and C. P. Verrijzer The Transcriptional Coactivator SAYP Is a Trithorax Group Signature Subunit of the PBAP Chromatin Remodeling Complex Mol. Cell. Biol., May 1, 2008; 28(9): 2920 - 2929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Gregory, C. R. Vakoc, T. Rozovskaia, X. Zheng, S. Patel, T. Nakamura, E. Canaani, and G. A. Blobel Mammalian ASH1L Is a Histone Methyltransferase That Occupies the Transcribed Region of Active Genes Mol. Cell. Biol., December 15, 2007; 27(24): 8466 - 8479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Beisel, A. Buness, I. M. Roustan-Espinosa, B. Koch, S. Schmitt, S. A. Haas, M. Hild, T. Katsuyama, and R. Paro Comparing active and repressed expression states of genes controlled by the Polycomb/Trithorax group proteins PNAS, October 16, 2007; 104(42): 16615 - 16620. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Secombe, L. Li, L. Carlos, and R. N. Eisenman The Trithorax group protein Lid is a trimethyl histone H3K4 demethylase required for dMyc-induced cell growth Genes & Dev., March 1, 2007; 21(5): 537 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Moshkin, L. Mohrmann, W. F. J. van Ijcken, and C. P. Verrijzer Functional Differentiation of SWI/SNF Remodelers in Transcription and Cell Cycle Control Mol. Cell. Biol., January 15, 2007; 27(2): 651 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. B. Zraly, F. A. Middleton, and A. K. Dingwall Hormone-response Genes Are Direct in Vivo Regulatory Targets of Brahma (SWI/SNF) Complex Function J. Biol. Chem., November 17, 2006; 281(46): 35305 - 35315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Papp and J. Muller Histone trimethylation and the maintenance of transcriptional ONand OFF states by trxG and PcG proteins Genes & Dev., August 1, 2006; 20(15): 2041 - 2054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Muller, Y. Oma, L. Vallar, E. Friederich, O. Poch, and B. Winsor Sequence and Comparative Genomic Analysis of Actin-related Proteins Mol. Biol. Cell, December 1, 2005; 16(12): 5736 - 5748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Muller, S. J. Kugler, A. Preiss, D. Maier, and A. C. Nagel Genetic Modifier Screens on Hairless Gain-of-Function Phenotypes Reveal Genes Involved in Cell Differentiation, Cell Growth and Apoptosis in Drosophila melanogaster Genetics, November 1, 2005; 171(3): 1137 - 1152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Armstrong, A. S. Sperling, R. Deuring, L. Manning, S. L. Moseley, O. Papoulas, C. I. Piatek, C. Q. Doe, and J. W. Tamkun Genetic Screens for Enhancers of brahma Reveal Functional Interactions Between the BRM Chromatin-Remodeling Complex and the Delta-Notch Signal Transduction Pathway in Drosophila Genetics, August 1, 2005; 170(4): 1761 - 1774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yan, K. Cui, D. M. Murray, C. Ling, Y. Xue, A. Gerstein, R. Parsons, K. Zhao, and W. Wang PBAF chromatin-remodeling complex requires a novel specificity subunit, BAF200, to regulate expression of selective interferon-responsive genes Genes & Dev., July 15, 2005; 19(14): 1662 - 1667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Srinivasan, J. A. Armstrong, R. Deuring, I. K. Dahlsveen, H. McNeill, and J. W. Tamkun The Drosophila trithorax group protein Kismet facilitates an early step in transcriptional elongation by RNA Polymerase II Development, April 1, 2005; 132(7): 1623 - 1635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Farrona, L. Hurtado, J. L. Bowman, and J. C. Reyes The Arabidopsis thaliana SNF2 homolog AtBRM controls shoot development and flowering Development, October 15, 2004; 131(20): 4965 - 4975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Angulo, M. Corominas, and F. Serras Activation and repression activities of ash2 in Drosophila wing imaginal discs Development, October 15, 2004; 131(20): 4943 - 4953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Kankel, D. M. Duncan, and I. Duncan A Screen for Genes That Interact With the Drosophila Pair-Rule Segmentation Gene fushi tarazu Genetics, September 1, 2004; 168(1): 161 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Zraly, D. R. Marenda, and A. K. Dingwall SNR1 (INI1/SNF5) Mediates Important Cell Growth Functions of the Drosophila Brahma (SWI/SNF) Chromatin Remodeling Complex Genetics, September 1, 2004; 168(1): 199 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Brumby, J. Secombe, J. Horsfield, M. Coombe, N. Amin, D. Coates, R. Saint, and H. Richardson A Genetic Screen for Dominant Modifiers of a cyclin E Hypomorphic Mutation Identifies Novel Regulators of S-Phase Entry in Drosophila Genetics, September 1, 2004; 168(1): 227 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kimura, M. Tada, N. Nakatsuji, and T. Tada Histone Code Modifications on Pluripotential Nuclei of Reprogrammed Somatic Cells Mol. Cell. Biol., July 1, 2004; 24(13): 5710 - 5720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Cheng and A. Shearn The Direct Interaction Between ASH2, a Drosophila Trithorax Group Protein, and SKTL, a Nuclear Phosphatidylinositol 4-Phosphate 5-Kinase, Implies a Role for Phosphatidylinositol 4,5-Bisphosphate in Maintaining Transcriptionally Active Chromatin Genetics, July 1, 2004; 167(3): 1213 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Sengupta, A. Kuhrs, and J. Muller General transcriptional silencing by a Polycomb response element in Drosophila Development, May 1, 2004; 131(9): 1959 - 1965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Mohrmann, K. Langenberg, J. Krijgsveld, A. J. Kal, A. J. R. Heck, and C. P. Verrijzer Differential Targeting of Two Distinct SWI/SNF-Related Drosophila Chromatin-Remodeling Complexes Mol. Cell. Biol., April 15, 2004; 24(8): 3077 - 3088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Janody, J. D. Lee, N. Jahren, D. J. Hazelett, A. Benlali, G. I. Miura, I. Draskovic, and J. E. Treisman A Mosaic Genetic Screen Reveals Distinct Roles for trithorax and Polycomb Group Genes in Drosophila Eye Development Genetics, January 1, 2004; 166(1): 187 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhang, Y. Jin, Y. Ji, J. Girton, J. Johansen, and K. M. Johansen Genetic and Phenotypic Analysis of Alleles of the Drosophila Chromosomal JIL-1 Kinase Reveals a Functional Requirement at Multiple Developmental Stages Genetics, November 1, 2003; 165(3): 1341 - 1354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. N. Byrd and A. Shearn ASH1, a Drosophila trithorax group protein, is required for methylation of lysine 4 residues on histone H3 PNAS, September 30, 2003; 100(20): 11535 - 11540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shimojima, M. Okada, T. Nakayama, H. Ueda, K. Okawa, A. Iwamatsu, H. Handa, and S. Hirose Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor Genes & Dev., July 1, 2003; 17(13): 1605 - 1616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Williams, Z. Li, S. Liu, E. V. Williams, G. Leung, T. J. Yen, and M. L. Goldberg Zwilch, a New Component of the ZW10/ROD Complex Required for Kinetochore Functions Mol. Biol. Cell, April 1, 2003; 14(4): 1379 - 1391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Beltran, E. Blanco, F. Serras, B. Perez-Villamil, R. Guigo, S. Artavanis-Tsakonas, and M. Corominas Transcriptional network controlled by the trithorax-group gene ash2 in Drosophila melanogaster PNAS, March 18, 2003; 100(6): 3293 - 3298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Poux, B. Horard, C. J. A. Sigrist, and V. Pirrotta The Drosophila Trithorax protein is a coactivator required to prevent re-establishment of Polycomb silencing Development, March 7, 2003; 129(10): 2483 - 2493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gutierrez, M. Zurita, J. A. Kennison, and M. Vazquez The Drosophila trithorax group gene tonalli(tna) interacts genetically with the Brahma remodeling complex and encodes an SP-RING finger protein Development, March 2, 2003; 130(2): 343 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Faucheux, J.-Y. Roignant, S. Netter, J. Charollais, C. Antoniewski, and L. Theodore batman Interacts with Polycomb and trithorax Group Genes and Encodes a BTB/POZ Protein That Is Included in a Complex Containing GAGA Factor Mol. Cell. Biol., February 15, 2003; 23(4): 1181 - 1195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Perrin, S. Bloyer, C. Ferraz, N. Agrawal, P. Sinha, and J. M. Dura The Leucine Zipper Motif of the Drosophila AF10 Homologue Can Inhibit PRE-Mediated Repression: Implications for Leukemogenic Activity of Human MLL-AF10 Fusions Mol. Cell. Biol., January 1, 2003; 23(1): 119 - 130. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-H. Goo, Y. C. Sohn, D.-H. Kim, S.-W. Kim, M.-J. Kang, D.-J. Jung, E. Kwak, N. A. Barlev, S. L. Berger, V. T. Chow, et al. Activating Signal Cointegrator 2 Belongs to a Novel Steady-State Complex That Contains a Subset of Trithorax Group Proteins Mol. Cell. Biol., January 1, 2003; 23(1): 140 - 149. [Abstract] [Full Text]
|
|
|
|
|
|
D. R. Marenda, C. B. Zraly, Y. Feng, S. Egan, and A. K. Dingwall The Drosophila SNR1 (SNF5/INI1) Subunit Directs Essential Developmental Functions of the Brahma Chromatin Remodeling Complex Mol. Cell. Biol., January 1, 2003; 23(1): 289 - 305. [Abstract] [Full Text]
|
|
|
|
|
|
I. F. G. King, N. J. Francis, and R. E. Kingston Native and Recombinant Polycomb Group Complexes Establish a Selective Block to Template Accessibility To Repress Transcription In Vitro Mol. Cell. Biol., November 15, 2002; 22(22): 7919 - 7928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Moshkin, J. A. Armstrong, R. K. Maeda, J. W. Tamkun, P. Verrijzer, J. A. Kennison, and F. Karch Histone chaperone ASF1 cooperates with the Brahma chromatin-remodelling machinery Genes & Dev., October 15, 2002; 16(20): 2621 - 2626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Maurange and R. Paro A cellular memory module conveys epigenetic inheritance of hedgehog expression during Drosophila wing imaginal disc development Genes & Dev., October 15, 2002; 16(20): 2672 - 2683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Olave, W. Wang, Y. Xue, A. Kuo, and G. R. Crabtree Identification of a polymorphic, neuron-specific chromatin remodeling complex Genes & Dev., October 1, 2002; 16(19): 2509 - 2517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Chai, J.-m. Hsu, J. Du, and B. C. Laurent Yeast RSC Function Is Required for Organization of the Cellular Cytoskeleton via an Alternative PKC1 Pathway Genetics, June 1, 2002; 161(2): 575 - 584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. D. Martini, S. Keeney, and M. A. Osley A Role for Histone H2B During Repair of UV-Induced DNA Damage in Saccharomyces cerevisiae Genetics, April 1, 2002; 160(4): 1375 - 1387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. J. Rando, K. Zhao, P. Janmey, and G. R. Crabtree Phosphatidylinositol-dependent actin filament binding by the SWI/SNF-like BAF chromatin remodeling complex PNAS, March 5, 2002; 99(5): 2824 - 2829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Pena-Rangel, I. Rodriguez, and J. R. Riesgo-Escovar A Misexpression Study Examining Dorsal Thorax Formation in Drosophila melanogaster Genetics, March 1, 2002; 160(3): 1035 - 1050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Zraly, Y. Feng, and A. K. Dingwall Genetic and Molecular Analysis of Region 88E9;88F2 in Drosophila melanogaster, Including the ear Gene Related to Human Factors Involved in Lineage-Specific Leukemias Genetics, March 1, 2002; 160(3): 1051 - 1065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kato, A. Tjernberg, W. Zhang, A. N. Krutchinsky, W. An, T. Takeuchi, Y. Ohtsuki, S. Sugano, D. R. de Bruijn, B. T. Chait, et al. SYT Associates with Human SNF/SWI Complexes and the C-terminal Region of Its Fusion Partner SSX1 Targets Histones J. Biol. Chem., February 8, 2002; 277(7): 5498 - 5505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Calgaro, M. Boube, D. L. Cribbs, and H.-M. Bourbon The Drosophila Gene taranis Encodes a Novel Trithorax Group Member Potentially Linked to the Cell Cycle Regulatory Apparatus Genetics, February 1, 2002; 160(2): 547 - 560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Nagy, J. Griesenbeck, R. D. Kornberg, and M. L. Cleary A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3 PNAS, December 13, 2001; (2001) 221596698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Kim, S.-O. Huh, H. Choi, K.-S. Lee, D. Shin, C. Lee, J.-S. Nam, H. Kim, H. Chung, H. W. Lee, et al. Srg3, a Mouse Homolog of Yeast SWI3, Is Essential for Early Embryogenesis and Involved in Brain Development Mol. Cell. Biol., November 15, 2001; 21(22): 7787 - 7795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. O'Connell, L. Wang, S. Robert, C. A. Jones, R. Saint, and R. S. Jones Polycomblike PHD Fingers Mediate Conserved Interaction with Enhancer of Zeste Protein J. Biol. Chem., November 9, 2001; 276(46): 43065 - 43073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xu, J. Paulsen, Y. Yoo, E. B. Goodwin, and S. Strome Caenorhabditis elegans MES-3 Is a Target of GLD-1 and Functions Epigenetically in Germline Development Genetics, November 1, 2001; 159(3): 1007 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Bajusz, L. Sipos, Z. Gyorgypal, E. A. Carrington, R. S. Jones, J. Gausz, and H. Gyurkovics The Trithorax-mimic Allele of Enhancer of zeste Renders Active Domains of Target Genes Accessible to Polycomb-Group-Dependent Silencing in Drosophila melanogaster Genetics, November 1, 2001; 159(3): 1135 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Fitzgerald and W. Bender Polycomb Group Repression Reduces DNA Accessibility Mol. Cell. Biol., October 1, 2001; 21(19): 6585 - 6597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-L. Chang, Y.-H. Peng, I-C. Pan, D.-S. Sun, B. King, and D.-H. Huang Essential role of Drosophila Hdac1 in homeotic gene silencing PNAS, August 1, 2001; (2001) 171325498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mollaaghababa, L. Sipos, S. Y. K. Tiong, O. Papoulas, J. A. Armstrong, J. W. Tamkun, and W. Bender Mutations in Drosophila heat shock cognate 4 are enhancers of Polycomb PNAS, March 27, 2001; 98(7): 3958 - 3963. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Scaerou, D. A. Starr, F. Piano, O. Papoulas, R. E. Karess, and M. L. Goldberg The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore J. Cell Sci., January 9, 2001; 114(17): 3103 - 3114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ruhf, A Braun, O Papoulas, J. Tamkun, N Randsholt, and M Meister The domino gene of Drosophila encodes novel members of the SWI2/SNF2 family of DNA-dependent ATPases, which contribute to the silencing of homeotic genes Development, January 4, 2001; 128(8): 1429 - 1441. [Abstract] [PDF]
|
|
|
|
|
|
J Treisman Drosophila homologues of the transcriptional coactivation complex subunits TRAP240 and TRAP230 are required for identical processes in eye-antennal disc development Development, January 2, 2001; 128(4): 603 - 615. [Abstract] [PDF]
|
|
|
|
|
|
M. Decoville, E. Giacomello, M. Leng, and D. Locker DSP1, an HMG-like Protein, Is Involved in the Regulation of Homeotic Genes Genetics, January 1, 2001; 157(1): 237 - 244. [Abstract] [Full Text]
|
|
|
|
|
|
F. Bantignies, R. H. Goodman, and S. M. Smolik Functional Interaction between the Coactivator Drosophila CREB-Binding Protein and ASH1, a Member of the Trithorax Group of Chromatin Modifiers Mol. Cell. Biol., December 15, 2000; 20(24): 9317 - 9330. [Abstract] [Full Text]
|
|
|
|
|
|
R. T. Collins and J. E. Treisman Osa-containing Brahma chromatin remodeling complexes are required for the repression of Wingless target genes Genes & Dev., December 15, 2000; 14(24): 3140 - 3152. [Abstract] [Full Text]
|
|
|
|
|
|
Z. Nie, Y. Xue, D. Yang, S. Zhou, B. J. Deroo, T. K. Archer, and W. Wang A Specificity and Targeting Subunit of a Human SWI/SNF Family-Related Chromatin-Remodeling Complex Mol. Cell. Biol., December 1, 2000; 20(23): 8879 - 8888. [Abstract] [Full Text]
|
|
|
|
|
|
J. J. Gildea, R. Lopez, and A. Shearn A Screen for New Trithorax Group Genes Identified little imaginal discs, the Drosophila melanogaster Homologue of Human Retinoblastoma Binding Protein 2 Genetics, October 1, 2000; 156(2): 645 - 663. [Abstract] [Full Text]
|
|
