|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 1 97-107, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
JD Thatcher, C Haun and PG Okkema
Department of Biological Sciences (M/C567), University of Illinois at Chicago, Chicago, IL 60607, USA.
Gene expression in the pharyngeal muscles of Caenorhabditis elegans is controlled in part by organ-specific signals, which in the myo-2 gene target a short DNA sequence termed the C subelement. To identify genes contributing to these signals, we performed a yeast one-hybrid screen for cDNAs encoding factors that bind the C subelement. One clone recovered was from daf-3, which encodes a Smad most closely related to vertebrate Smad4. We demonstrated that DAF-3 binds C subelement DNA directly and specifically using gel mobility shift and DNase1 protection assays. Mutation of any base in the sequence GTCTG interfered with binding in the gel mobility shift assay, demonstrating that this pentanucleotide is a core recognition sequence for DAF-3 binding. daf-3 is known to promote formation of dauer larvae and this activity is negatively regulated by TGFbeta-like signaling. To determine how daf-3 affects C subelement enhancer activity in vivo, we examined expression a gfp reporter controlled by a concatenated C subelement oligonucleotide in daf-3 mutants and other mutants affecting the TGFbeta-like signaling pathway controlling dauer formation. Our results demonstrate that wild-type daf-3 can repress C subelement enhancer activity during larval development and, like its dauer-promoting activity, daf-3's repressor activity is negatively regulated by TGFbeta-like signaling. We have examined expression of this gfp reporter in dauer larvae and have observed no daf-3-dependent repression of C activity. These results suggest daf-3 directly regulates pharyngeal gene expression during non-dauer development.
This article has been cited by other articles:
|
|
 |
|
  H. Lans and G. Jansen Noncell- and Cell-Autonomous G-Protein-Signaling Converges With Ca2+/Mitogen-Activated Protein Kinase Signaling to Regulate str-2 Receptor Gene Expression in Caenorhabditis elegans Genetics, July 1, 2006; 173(3): 1287 - 1299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Bates, M. Victor, A. K. Jones, Y. Shi, and A. C. Hart Differential contributions of Caenorhabditis elegans histone deacetylases to huntingtin polyglutamine toxicity. J. Neurosci., March 8, 2006; 26(10): 2830 - 2838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. T. Takaesu, E. Herbig, D. Zhitomersky, M. B. O'Connor, and S. J. Newfeld DNA-binding domain mutations in SMAD genes yield dominant-negative proteins or a neomorphic protein that can activate WG target genes in Drosophila Development, November 1, 2005; 132(21): 4883 - 4894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Deplancke, D. Dupuy, M. Vidal, and A. J.M. Walhout A Gateway-Compatible Yeast One-Hybrid System Genome Res., October 1, 2004; 14(10b): 2093 - 2101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Ao, J. Gaudet, W. J. Kent, S. Muttumu, and S. E. Mango Environmentally Induced Foregut Remodeling by PHA-4/FoxA and DAF-12/NHR Science, September 17, 2004; 305(5691): 1743 - 1746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. da Graca, K. K. Zimmerman, M. C. Mitchell, M. Kozhan-Gorodetska, K. Sekiewicz, Y. Morales, and G. I. Patterson,, DAF-5 is a Ski oncoprotein homolog that functions in a neuronal TGF{beta} pathway to regulate C. elegans dauer development Development, January 15, 2004; 131(2): 435 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. Peckol, E. R. Troemel, and C. I. Bargmann Sensory experience and sensory activity regulate chemosensory receptor gene expression in Caenorhabditis elegans PNAS, September 25, 2001; 98(20): 11032 - 11038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Praitis, E. Casey, D. Collar, and J. Austin Creation of Low-Copy Integrated Transgenic Lines in Caenorhabditis elegans Genetics, March 1, 2001; 157(3): 1217 - 1226. [Abstract] [Full Text]
|
|
|
|
|
|
T. Inoue and J. H. Thomas Suppressors of Transforming Growth Factor-{beta} Pathway Mutants in the Caenorhabditis elegans Dauer Formation Pathway Genetics, November 1, 2000; 156(3): 1035 - 1046. [Abstract] [Full Text]
|
|
|
|
|
|
M. Ailion and J. H. Thomas Dauer Formation Induced by High Temperatures in Caenorhabditis elegans Genetics, November 1, 2000; 156(3): 1047 - 1067. [Abstract] [Full Text]
|
|
|
|
|
|
S. A. Daniels, M. Ailion, J. H. Thomas, and P. Sengupta egl-4 Acts Through a Transforming Growth Factor-{beta}/SMAD Pathway in Caenorhabditis elegans to Regulate Multiple Neuronal Circuits in Response to Sensory Cues Genetics, September 1, 2000; 156(1): 123 - 141. [Abstract] [Full Text]
|
|
|
|
|
|
S. J. Newfeld, R. G. Wisotzkey, and S. Kumar Molecular Evolution of a Developmental Pathway: Phylogenetic Analyses of Transforming Growth Factor-ß Family Ligands, Receptors and Smad Signal Transducers Genetics, June 1, 1999; 152(2): 783 - 795. [Abstract] [Full Text]
|
|
|
|
|
|
A Jazwinska, C Rushlow, and S Roth The role of brinker in mediating the graded response to Dpp in early Drosophila embryos Development, January 8, 1999; 126(15): 3323 - 3334. [Abstract] [PDF]
|
|
|
|
 |
|
 K. A. Henningfeld, S. Rastegar, G. Adler, and W. Knochel Smad1 and Smad4 Are Components of the Bone Morphogenetic Protein-4 (BMP-4)-induced Transcription Complex of the Xvent-2B Promoter J. Biol. Chem., July 14, 2000; 275(29): 21827 - 21835. [Abstract] [Full Text] [PDF]
|
|
