|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 10 2215-2225, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
O Pabst, R Zweigerdt and HH Arnold
Department of Cell and Molecular Biology, University of Braunschweig, Spielmannstrasse 7, Germany.
The homeodomain transcription factor Nkx2-3 is expressed in gut mesenchyme and spleen of embryonic and adult mice. Targeted inactivation of the Nkx2-3 gene results in severe morphological alterations of both organs and early postnatal lethality in the majority of homozygous mutants. Villus formation in the small intestine appears considerably delayed in Nkx2-3(-)/- foetuses due to reduced proliferation of the epithelium, while massively increased growth of crypt cells ensues in surviving adult mutants. Interestingly, differentiated cell types of the intestinal epithelium are present in homozygous mutants, suggesting that Nkx2-3 is not required for their cell lineage allocation or migration-dependent differentiation. Hyperproliferation of the gut epithelium in adult mutants is associated with markedly reduced expression of BMP-2 and BMP-4, suggesting that these signalling molecules may be involved in mediating non-cell-autonomous control of intestinal cell growth. Spleens of Nkx2-3 mutants are generally smaller and contain drastically reduced numbers of lymphatic cells. The white pulp appears anatomically disorganized, possibly owing to a homing defect in the spleen parenchyme. Moreover, some of the Nkx2-3 mutants exhibit asplenia. Taken together these observations indicate that Nkx2-3 is essential for normal development and functions of the small intestine and spleen.
This article has been cited by other articles:
|
|
 |
|
  M. J. Geske, X. Zhang, K. K. Patel, D. M. Ornitz, and T. S. Stappenbeck Fgf9 signaling regulates small intestinal elongation and mesenchymal development Development, September 1, 2008; 135(17): 2959 - 2968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Y. Choi, A. I. Romer, Y. Wang, M. P. Wu, S. Ito, A. B. Leiter, and R. A. Shivdasani Requirement of the Tissue-Restricted Homeodomain Transcription Factor Nkx6.3 in Differentiation of Gastrin-Producing G Cells in the Stomach Antrum Mol. Cell. Biol., May 15, 2008; 28(10): 3208 - 3218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, B. B. Madison, W. Zacharias, A. Kolterud, D. States, and D. L. Gumucio Deconvoluting the intestine: molecular evidence for a major role of the mesenchyme in the modulation of signaling cross talk Physiol Genomics, May 11, 2007; 29(3): 290 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Asayesh, J. Sharpe, R. P. Watson, J. Hecksher-Sorensen, N. D. Hastie, R. E. Hill, and U. Ahlgren Spleen versus pancreas: strict control of organ interrelationship revealed by analyses of Bapx1-/- mice. Genes & Dev., August 15, 2006; 20(16): 2208 - 2213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Seymour, J. P. Sundberg, and H. HogenEsch Abnormal lymphoid organ development in immunodeficient mutant mice. Vet. Pathol., July 1, 2006; 43(4): 401 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Brendolan, E. Ferretti, V. Salsi, K. Moses, S. Quaggin, F. Blasi, M. L. Cleary, and L. Selleri A Pbx1-dependent genetic and transcriptional network regulates spleen ontogeny Development, July 1, 2005; 132(13): 3113 - 3126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gregorieff and H. Clevers Wnt signaling in the intestinal epithelium: from endoderm to cancer Genes & Dev., April 15, 2005; 19(8): 877 - 890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Z. Rizvi and M. H. Wong Epithelial Stem Cells and Their Niche: There's No Place Like Home Stem Cells, February 1, 2005; 23(2): 150 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. V. O. Hansen, N. A. Hammer, J. Nielsen, M. Madsen, C. Dalbaeck, U. M. Wewer, J. Christiansen, and F. C. Nielsen Dwarfism and Impaired Gut Development in Insulin-Like Growth Factor II mRNA-Binding Protein 1-Deficient Mice Mol. Cell. Biol., May 15, 2004; 24(10): 4448 - 4464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Tarlinton, A. Light, D. Metcalf, R. P. Harvey, and L. Robb Architectural Defects in the Spleens of Nkx2-3-Deficient Mice Are Intrinsic and Associated with Defects in Both B Cell Maturation and T Cell-Dependent Immune Responses J. Immunol., April 15, 2003; 170(8): 4002 - 4010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F Beck Homeobox genes in gut development Gut, September 1, 2002; 51(3): 450 - 454. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. S. Solorzano-Vargas, J. Wang, L. Jiang, H. V. Tsai, L. O. Ontiveros, M. A. Vazir, R. J. Aguilera, and M. G. Martin Multiple transcription factors in 5'-flanking region of human polymeric Ig receptor control its basal expression Am J Physiol Gastrointest Liver Physiol, August 1, 2002; 283(2): G415 - G425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Yang, N. A. Bermingham, M. J. Finegold, and H. Y. Zoghbi Requirement of Math1 for Secretory Cell Lineage Commitment in the Mouse Intestine Science, December 7, 2001; 294(5549): 2155 - 2158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Perreault, J. P. Katz, S. D. Sackett, and K. H. Kaestner Foxl1 Controls the Wnt/beta -Catenin Pathway by Modulating the Expression of Proteoglycans in the Gut J. Biol. Chem., November 9, 2001; 276(46): 43328 - 43333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Mills and J. I. Gordon The intestinal stem cell niche: There grows the neighborhood PNAS, October 23, 2001; 98(22): 12334 - 12336. [Full Text] [PDF]
|
|
|
|
|
|
F. Calabi, R. Pannell, and G. Pavloska Gene Targeting Reveals a Crucial Role for MTG8 in the Gut Mol. Cell. Biol., August 15, 2001; 21(16): 5658 - 5666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lu, P. Chang, J. A. Richardson, L. Gan, H. Weiler, and E. N. Olson Inaugural Article: The basic helix-loop-helix transcription factor capsulin controls spleen organogenesis PNAS, August 15, 2000; 97(17): 9525 - 9530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tanaka, N. Yamasaki, and S. Izumo Phenotypic Characterization of the Murine Nkx2.6 Homeobox Gene by Gene Targeting Mol. Cell. Biol., April 15, 2000; 20(8): 2874 - 2879. [Abstract] [Full Text]
|
|
|
|
|
|
L Karlsson, P Lindahl, J. Heath, and C Betsholtz Abnormal gastrointestinal development in PDGF-A and PDGFR-(alpha) deficient mice implicates a novel mesenchymal structure with putative instructive properties in villus morphogenesis Development, January 8, 2000; 127(16): 3457 - 3466. [Abstract] [PDF]
|
|
|
|
|
|
S. J. Baek, J. M. Horowitz, and T. E. Eling Molecular Cloning and Characterization of Human Nonsteroidal Anti-inflammatory Drug-activated Gene Promoter. BASAL TRANSCRIPTION IS MEDIATED BY Sp1 AND Sp3 J. Biol. Chem., August 31, 2001; 276(36): 33384 - 33392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Phiel, V. Gabbeta, L. M. Parsons, D. Rothblat, R. P. Harvey, and K. M. McHugh Differential Binding of an SRF/NK-2/MEF2 Transcription Factor Complex in Normal Versus Neoplastic Smooth Muscle Tissues J. Biol. Chem., September 7, 2001; 276(37): 34637 - 34650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Wong, J. R. Saam, T. S. Stappenbeck, C. H. Rexer, and J. I. Gordon Genetic mosaic analysis based on Cre recombinase and navigated laser capture microdissection PNAS, November 7, 2000; 97(23): 12601 - 12606. [Abstract] [Full Text] [PDF]
|
|
