|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 13 3005-3014, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
C Takke and JA Campos-Ortega
Institut fur Entwicklungsbiologie, Universitat zu Koln, Germany.
During vertebrate embryonic development, the paraxial mesoderm becomes subdivided into metameric units known as somites. In the zebrafish embryo, genes encoding homologues of the proteins of the Drosophila Notch signalling pathway are expressed in the presomitic mesoderm and expression is maintained in a segmental pattern during somitogenesis. This expression pattern suggests a role for these genes during somite development. We misexpressed various zebrafish genes of this group by injecting mRNA into early embryos. RNA encoding a constitutively active form of notch1a (notch1a-intra) and a truncated variant of deltaD [deltaD(Pst)], as well as transcripts of deltaC and deltaD, the hairy-E(spl) homologues her1 and her4, and groucho2 were tested for their effects on somite formation, myogenesis and on the pattern of transcription of putative downstream genes. In embryos injected with any of these RNAs, with the exception of groucho2 RNA, the paraxial mesoderm differentiated normally into somitic tissue, but failed to segment correctly. Activation of notch results in ectopic activation of her1 and her4. This misregulation of the expression of her genes might be causally related to the observed mesodermal defects, as her1 and her4 mRNA injections led to effects similar to those seen with notch1a-intra. deltaC and deltaD seem to function after subdivision of the presomitic mesoderm, since the her gene transcription pattern in the presomitic mesoderm remains essentially normal after misexpression of delta genes. Whereas notch signalling alone apparently does not affect myogenesis, zebrafish groucho2 is involved in differentiation of mesodermal derivatives.
This article has been cited by other articles:
|
|
 |
|
  V. Bolos, J. Grego-Bessa, and J. L. de la Pompa Notch Signaling in Development and Cancer Endocr. Rev., May 1, 2007; 28(3): 339 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Schuster-Gossler, R. Cordes, and A. Gossler Premature myogenic differentiation and depletion of progenitor cells cause severe muscle hypotrophy in Delta1 mutants PNAS, January 9, 2007; 104(2): 537 - 542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Holley Anterior-posterior differences in vertebrate segments: specification of trunk and tail somites in the zebrafish blastula Genes & Dev., July 15, 2006; 20(14): 1831 - 1837. [Full Text] [PDF]
|
|
|
|
|
|
A. Kawamura, S. Koshida, H. Hijikata, T. Sakaguchi, H. Kondoh, and S. Takada Zebrafish Hairy/Enhancer of split protein links FGF signaling to cyclic gene expression in the periodic segmentation of somites Genes & Dev., May 15, 2005; 19(10): 1156 - 1161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-K. Bae, T. Shimizu, and M. Hibi Patterning of proneuronal and inter-proneuronal domains by hairy- and enhancer of split-related genes in zebrafish neuroectoderm Development, March 15, 2005; 132(6): 1375 - 1385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Aerne and D. Ish-Horowicz receptor tyrosine phosphatase {psi} is required for Delta/Notch signalling and cyclic gene expression in the presomitic mesoderm Development, July 15, 2004; 131(14): 3391 - 3399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hans, N. Scheer, I. Riedl, E. v. Weizsacker, P. Blader, and J. A. Campos-Ortega her3, a zebrafish member of the hairy-E(spl) family, is repressed by Notch signalling Development, June 15, 2004; 131(12): 2957 - 2969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pasini, Y.-J. Jiang, and D. G. Wilkinson Two zebrafish Notch-dependent hairy/Enhancer-of-split-related genes, her6 and her4, are required to maintain the coordination of cyclic gene expression in the presomitic mesoderm Development, April 1, 2004; 131(7): 1529 - 1541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takahashi, T. Inoue, A. Gossler, and Y. Saga Feedback loops comprising Dll1, Dll3 and Mesp2, and differential involvement of Psen1 are essential for rostrocaudal patterning of somites Development, September 15, 2003; 130(18): 4259 - 4268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gajewski, D. Sieger, B. Alt, C. Leve, S. Hans, C. Wolff, K. B. Rohr, and D. Tautz Anterior and posterior waves of cyclic her1 gene expression are differentially regulated in the presomitic mesoderm of zebrafish Development, September 15, 2003; 130(18): 4269 - 4278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Winkler, M. Schafer, J. Duschl, M. Schartl, and J.-N. Volff Functional Divergence of Two Zebrafish Midkine Growth Factors Following Fish-Specific Gene Duplication Genome Res., June 1, 2003; 13(6): 1067 - 1081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-K. Bae, T. Shimizu, T. Yabe, C.-H. Kim, T. Hirata, H. Nojima, O. Muraoka, T. Hirano, and M. Hibi A homeobox gene, pnx, is involved in the formation of posterior neurons in zebrafish Development, May 1, 2003; 130(9): 1853 - 1865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Serth, K. Schuster-Gossler, R. Cordes, and A. Gossler Transcriptional oscillation of Lunatic fringe is essential for somitogenesis Genes & Dev., April 1, 2003; 17(7): 912 - 925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Oates and R. K. Ho Hairy/E(spl)-related (Her) genes are central components of the segmentation oscillator and display redundancy with the Delta/Notch signaling pathway in the formation of anterior segmental boundaries in the zebrafish Development, March 8, 2003; 129(12): 2929 - 2946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sato, K. Yasuda, and Y. Takahashi Morphological boundary forms by a novel inductive event mediated by Lunatic fringe and Notch during somitic segmentation Development, August 1, 2002; 129(15): 3633 - 3644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Henry, M. K. Urban, K. K. Dill, J. P. Merlie, M. F. Page, C. B. Kimmel, and S. L. Amacher Two linked hairy/Enhancer of split-related zebrafish genes, her1 and her7, function together to refine alternating somite boundaries Development, August 1, 2002; 129(15): 3693 - 3704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cossins, A. E. Vernon, Y. Zhang, A. Philpott, and P. H. Jones Hes6 regulates myogenic differentiation Development, January 5, 2002; 129(9): 2195 - 2207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Holley, D. Julich, G.-J. Rauch, R. Geisler, and C. Nusslein-Volhard her1 and the notch pathway function within the oscillator mechanism that regulates zebrafish somitogenesis Development, January 3, 2002; 129(5): 1175 - 1183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Topczewska, J. Topczewski, A. Shostak, T. Kume, L. Solnica-Krezel, and B. L.M. Hogan The winged helix transcription factor Foxc1a is essential for somitogenesis in zebrafish Genes & Dev., September 15, 2001; 15(18): 2483 - 2493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Bally-Cuif, C. Goutel, M. Wassef, W. Wurst, and F. Rosa Coregulation of anterior and posterior mesendodermal development by a hairy-related transcriptional repressor Genes & Dev., July 1, 2000; 14(13): 1664 - 1677. [Abstract] [Full Text]
|
|
|
|
|
|
S. A. Holley, R. Geisler, and C. Nüsslein-Volhard Control of her1 expression during zebrafish somitogenesis by a Delta-dependent oscillator and an independent wave-front activity Genes & Dev., July 1, 2000; 14(13): 1678 - 1690. [Abstract] [Full Text]
|
|
|
|
|
|
N Koyano-Nakagawa, J Kim, D Anderson, and C Kintner Hes6 acts in a positive feedback loop with the neurogenins to promote neuronal differentiation Development, January 10, 2000; 127(19): 4203 - 4216. [Abstract] [PDF]
|
|
|
|
|
|
L Durbin, P Sordino, A Barrios, M Gering, C Thisse, B Thisse, C Brennan, A Green, S Wilson, and N Holder Anteroposterior patterning is required within segments for somite boundary formation in developing zebrafish Development, January 4, 2000; 127(8): 1703 - 1713. [Abstract] [PDF]
|
|
