|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 14 3057-3065, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
J Zhou, H Ashe, C Burks and M Levine
Dept Mol. Cell Biol., Division of Genetics, University of California, Berkeley, CA 94720, USA. mlevine@uclink4.berkeley.edu
Genetic studies have identified an unusual transvection process in the Abdominal-B (Abd-B) locus of Drosophila. In some cases distal infraabdominal (iab) regulatory domains continue to activate the Abd-B promoter even when translocated onto different chromosomes. Transvection depends on an approx. 10 kb genomic DNA sequence, termed the transvection mediating region (tmr), located immediately downstream of the Abd-B transcription unit. Here we report a detailed analysis of this region. Different DNA fragments from the tmr were inserted into a variety of P-transformation vectors. Analyses of reporter gene expression in transgenic embryos and adults identify at least three cis-regulatory elements, including two enhancers (IAB7 and IAB8) and a new insulator DNA (Frontabdominal-8, Fab-8). Evidence is also presented for a Polycomb Response Element (PRE) linked to the IAB8 enhancer, and an internal promoter in the iab-8 domain, which transcribes the iab-7 and iab-8 cis-regulatory DNA, including the Fab-8 insulator. We discuss the significance of these findings with regard to Abd-B transvection and long-range enhancer-promoter interactions in mammalian globin loci.
This article has been cited by other articles:
|
|
 |
|
  O. Kyrchanova, S. Toshchakov, Y. Podstreshnaya, A. Parshikov, and P. Georgiev Functional Interaction between the Fab-7 and Fab-8 Boundaries and the Upstream Promoter Region in the Drosophila Abd-B Gene Mol. Cell. Biol., June 15, 2008; 28(12): 4188 - 4195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Bender MicroRNAs in the Drosophila bithorax complex Genes & Dev., January 1, 2008; 22(1): 14 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. S. Akbari, E. Bae, H. Johnsen, A. Villaluz, D. Wong, and R. A. Drewell A novel promoter-tethering element regulates enhancer-driven gene expression at the bithorax complex in the Drosophila embryo Development, January 1, 2008; 135(1): 123 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Schneider and R. Grosschedl Dynamics and interplay of nuclear architecture, genome organization, and gene expression Genes & Dev., December 1, 2007; 21(23): 3027 - 3043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Rodin, O. Kyrchanova, E. Pomerantseva, A. Parshikov, and P. Georgiev New Properties of Drosophila Fab-7 Insulator Genetics, September 1, 2007; 177(1): 113 - 121. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Chen, L. Lin, S. Smith, J. Huang, S. L. Berger, and J. Zhou CTCF-Dependent Chromatin Boundary Element between the Latency-Associated Transcript and ICP0 Promoters in the Herpes Simplex Virus Type 1 Genome J. Virol., May 15, 2007; 81(10): 5192 - 5201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Kyrchanova, S. Toshchakov, A. Parshikov, and P. Georgiev Study of the Functional Interaction between Mcp Insulators from the Drosophila bithorax Complex: Effects of Insulator Pairing on Enhancer-Promoter Communication Mol. Cell. Biol., April 15, 2007; 27(8): 3035 - 3043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Lin, Q. Chen, L. Lin, S. Smith, and J. Zhou Promoter targeting sequence mediates enhancer interference in the Drosophila embryo PNAS, February 27, 2007; 104(9): 3237 - 3242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mihaly, S. Barges, L. Sipos, R. Maeda, F. Cleard, I. Hogga, W. Bender, H. Gyurkovics, and F. Karch Dissecting the regulatory landscape of the Abd-B gene of the bithorax complex Development, August 1, 2006; 133(15): 2983 - 2993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Maeda and F. Karch The ABC of the BX-C: the bithorax complex explained. Development, April 1, 2006; 133(8): 1413 - 1422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Golovnin, E. Melnick, A. Mazur, and P. Georgiev Drosophila Su(Hw) Insulator Can Stimulate Transcription of a Weakened yellow Promoter Over a Distance Genetics, July 1, 2005; 170(3): 1133 - 1142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gruzdeva, O. Kyrchanova, A. Parshikov, A. Kullyev, and P. Georgiev The Mcp Element from the bithorax Complex Contains an Insulator That Is Capable of Pairwise Interactions and Can Facilitate Enhancer-Promoter Communication Mol. Cell. Biol., May 1, 2005; 25(9): 3682 - 3689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Lin, Q. Chen, L. Lin, and J. Zhou The Promoter Targeting Sequence mediates epigenetically heritable transcription memory Genes & Dev., November 1, 2004; 18(21): 2639 - 2651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. C. Calhoun and M. Levine Long-range enhancer-promoter interactions in the Scr-Antp interval of the Drosophila Antennapedia complex PNAS, August 19, 2003; 100(17): 9878 - 9883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Golovnin, I. Biryukova, O. Romanova, M. Silicheva, A. Parshikov, E. Savitskaya, V. Pirrotta, and P. Georgiev An endogenous Su(Hw) insulator separates the yellow gene from the Achaete-scute gene complex in Drosophila Development, July 15, 2003; 130(14): 3249 - 3258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nowling, C. Bernadt, L. Johnson, M. Desler, and A. Rizzino The Co-activator p300 Associates Physically with and Can Mediate the Action of the Distal Enhancer of the FGF-4 Gene J. Biol. Chem., April 11, 2003; 278(16): 13696 - 13705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Savitsky, T. Kahn, E. Pomerantseva, and P. Georgiev Transvection at the End of the Truncated Chromosome in Drosophila melanogaster Genetics, April 1, 2003; 163(4): 1375 - 1387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Estrada, F. Casares, A. Busturia, and E. Sanchez-Herrero Genetic and molecular characterization of a novel iab-8 regulatory domain in the Abdominal-B gene of Drosophila melanogaster Development, March 13, 2003; 129(22): 5195 - 5204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Lin, D. Wu, and J. Zhou The promoter targeting sequence facilitates and restricts a distant enhancer to a single promoter in the Drosophila embryo Development, February 1, 2003; 130(3): 519 - 526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Bae, V. C. Calhoun, M. Levine, E. B. Lewis, and R. A. Drewell Characterization of the intergenic RNA profile at abdominal-A and Abdominal-B in the Drosophila bithorax complex PNAS, December 24, 2002; 99(26): 16847 - 16852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Drewell, E. Bae, J. Burr, and E. B. Lewis Transcription defines the embryonic domains of cis-regulatory activity at the Drosophila bithorax complex PNAS, December 24, 2002; 99(26): 16853 - 16858. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Rank, M. Prestel, and R. Paro Transcription through Intergenic Chromosomal Memory Elements of the Drosophila Bithorax Complex Correlates with an Epigenetic Switch Mol. Cell. Biol., November 15, 2002; 22(22): 8026 - 8034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Melnikova, M. Gause, and P. Georgiev The gypsy Insulators Flanking yellow Enhancers Do Not Form a Separate Transcriptional Domain in Drosophila melanogaster: The Enhancers Can Activate an Isolated yellow Promoter Genetics, April 1, 2002; 160(4): 1549 - 1560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. West, M. Gaszner, and G. Felsenfeld Insulators: many functions, many mechanisms Genes & Dev., February 1, 2002; 16(3): 271 - 288. [Full Text] [PDF]
|
|
|
|
|
|
M. Kmita, B. Tarchini, D. Duboule, and Y. Herault Evolutionary conserved sequences are required for the insulation of the vertebrate Hoxd complex in neural cells Development, January 12, 2002; 129(23): 5521 - 5528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Hogga and F. Karch Transcription through the iab-7 cis-regulatory domain of the bithorax complex interferes with maintenance of Polycomb-mediated silencing Development, January 11, 2002; 129(21): 4915 - 4922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Bender and D. P. Fitzgerald Transcription activates repressed domains in the Drosophila bithorax complex Development, January 11, 2002; 129(21): 4923 - 4930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Drewell, K. L. Arney, T. Arima, S. C. Barton, J. D. Brenton, and M. A. Surani Novel conserved elements upstream of the H19 gene are transcribed and act as mesodermal enhancers Development, January 3, 2002; 129(5): 1205 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-K. Sang and D. F. Ready Eyes closed, a Drosophila p47 homolog, is essential for photoreceptor morphogenesis Development, January 1, 2002; 129(1): 143 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. N. Cai, Z. Zhang, J. R. Adams, and P. Shen Genomic context modulates insulator activity through promoter competition Development, November 1, 2001; 128(21): 4339 - 4347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. N. Cai and P. Shen Effects of cis Arrangement of Chromatin Insulators on Enhancer-Blocking Activity Science, January 19, 2001; 291(5503): 493 - 495. [Abstract] [Full Text]
|
|
|
|
|
|
D Beuchle, G Struhl, and J Muller Polycomb group proteins and heritable silencing of Drosophila Hox genes Development, January 3, 2001; 128(6): 993 - 1004. [Abstract] [PDF]
|
|
|
|
|
|
S Barges, J Mihaly, M Galloni, K Hagstrom, M Muller, G Shanower, P Schedl, H Gyurkovics, and F Karch The Fab-8 boundary defines the distal limit of the bithorax complex iab-7 domain and insulates iab-7 from initiation elements and a PRE in the adjacent iab-8 domain Development, January 2, 2000; 127(4): 779 - 790. [Abstract] [PDF]
|
|
