|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 126, Issue 14 3131-3148, Copyright © 1999 by Company of Biologists
JOURNAL ARTICLES |
MK Anderson, G Hernandez-Hoyos, RA Diamond and EV Rothenberg
Stowers Institute for Medical Research, Kansas City, MO 64110, USA. evroth@its.caltech.edu
Ets family transcription factors control the expression of a large number of genes in hematopoietic cells. Here we show strikingly precise differential expression of a subset of these genes marking critical, early stages of mouse lymphocyte cell-type specification. Initially, the Ets family member factor Erg was identified during an arrayed cDNA library screen for genes encoding transcription factors expressed specifically during T cell lineage commitment. Multiparameter fluorescence-activated cell sorting for over a dozen cell surface markers was used to isolate 18 distinct primary-cell populations representing discrete T cell and B cell developmental stages, pluripotent lymphoid precursors, immature NK-like cells and myeloid hematopoietic cells. These populations were monitored for mRNA expression of the Erg, Ets-1, Ets-2, Fli-1, Tel, Elf-1, GABPalpha, PU.1 and Spi-B genes. The earliest stages in T cell differentiation show particularly dynamic Ets family gene regulation, with sharp transitions in expression correlating with specification and commitment events. Ets, Spi-B and PU.1 are expressed in these stages but not by later T-lineage cells. Erg is induced during T-lineage specification and then silenced permanently, after commitment, at the beta-selection checkpoint. Spi-B is transiently upregulated during commitment and then silenced at the same stage as Erg. T-lineage commitment itself is marked by repression of PU.1, a factor that regulates B-cell and myeloid genes. These results show that the set of Ets factors mobilized during T-lineage specification and commitment is different from the set that maintains T cell gene expression during thymocyte repertoire selection and in all classes of mature T cells.
This article has been cited by other articles:
|
|
 |
|
  E. C. Torchia, K. Boyd, J. E. Rehg, C. Qu, and S. J. Baker EWS/FLI-1 Induces Rapid Onset of Myeloid/Erythroid Leukemia in Mice Mol. Cell. Biol., November 15, 2007; 27(22): 7918 - 7934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hoogenkamp, H. Krysinska, R. Ingram, G. Huang, R. Barlow, D. Clarke, A. Ebralidze, P. Zhang, H. Tagoh, P. N. Cockerill, et al. The Pu.1 Locus Is Differentially Regulated at the Level of Chromatin Structure and Noncoding Transcription by Alternate Mechanisms at Distinct Developmental Stages of Hematopoiesis Mol. Cell. Biol., November 1, 2007; 27(21): 7425 - 7438. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Baldus, P. Martus, T. Burmeister, S. Schwartz, N. Gokbuget, C. D. Bloomfield, D. Hoelzer, E. Thiel, and W. K. Hofmann Low ERG and BAALC Expression Identifies a New Subgroup of Adult Acute T-Lymphoblastic Leukemia With a Highly Favorable Outcome J. Clin. Oncol., August 20, 2007; 25(24): 3739 - 3745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Tydell, E.-S. David-Fung, J. E. Moore, L. Rowen, T. Taghon, and E. V. Rothenberg Molecular Dissection of Prethymic Progenitor Entry into the T Lymphocyte Developmental Pathway J. Immunol., July 1, 2007; 179(1): 421 - 438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Higuchi, F. O. Bartel, M. Masuya, T. Deguchi, K. W. Henderson, R. Li, R. C. Muise-Helmericks, M. J. Kern, D. K. Watson, and D. D. Spyropoulos Thymomegaly, Microsplenia, and Defective Homeostatic Proliferation of Peripheral Lymphocytes in p51-Ets1 Isoform-Specific Null Mice Mol. Cell. Biol., May 1, 2007; 27(9): 3353 - 3366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Baldus, T. Burmeister, P. Martus, S. Schwartz, N. Gokbuget, C. D. Bloomfield, D. Hoelzer, E. Thiel, and W. K. Hofmann High Expression of the ETS Transcription Factor ERG Predicts Adverse Outcome in Acute T-Lymphoblastic Leukemia in Adults J. Clin. Oncol., October 10, 2006; 24(29): 4714 - 4720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Pang, H.-H. Xue, G. Szalai, X. Wang, Y. Wang, D. K. Watson, W. J. Leonard, G. A. Blobel, and M. Poncz Maturation stage-specific regulation of megakaryopoiesis by pointed-domain Ets proteins Blood, October 1, 2006; 108(7): 2198 - 2206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. B. Franco, D. D. Scripture-Adams, I. Proekt, T. Taghon, A. H. Weiss, M. A. Yui, S. L. Adams, R. A. Diamond, and E. V. Rothenberg Notch/Delta signaling constrains reengineering of pro-T cells by PU.1 PNAS, August 8, 2006; 103(32): 11993 - 11998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Clements, S. A. John, and L. A. Garrett-Sinha Impaired Generation of CD8+ Thymocytes in Ets-1-Deficient Mice J. Immunol., July 15, 2006; 177(2): 905 - 912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Y. Wai, Z. Mi, C. Gao, H. Guo, C. Marroquin, and P. C. Kuo Ets-1 and Runx2 Regulate Transcription of a Metastatic Gene, Osteopontin, in Murine Colorectal Cancer Cells J. Biol. Chem., July 14, 2006; 281(28): 18973 - 18982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wang, C. L. Claus, G. Vaccarelli, M. Braunstein, T. M. Schmitt, J. C. Zuniga-Pflucker, E. V. Rothenberg, and M. K. Anderson The Basic Helix-Loop-Helix Transcription Factor HEBAlt Is Expressed in Pro-T Cells and Enhances the Generation of T Cell Precursors J. Immunol., July 1, 2006; 177(1): 109 - 119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. de Pooter, T. M. Schmitt, J. L. de la Pompa, Y. Fujiwara, S. H. Orkin, and J. C. Zuniga-Pflucker Notch Signaling Requires GATA-2 to Inhibit Myelopoiesis from Embryonic Stem Cells and Primary Hemopoietic Progenitors J. Immunol., May 1, 2006; 176(9): 5267 - 5275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-d. Geng and W. V. Vedeckis c-Myb and Members of the c-Ets Family of Transcription Factors Act as Molecular Switches to Mediate Opposite Steroid Regulation of the Human Glucocorticoid Receptor 1A Promoter J. Biol. Chem., December 30, 2005; 280(52): 43264 - 43271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Garcia-Ojeda, S. Dejbakhsh-Jones, D. Chatterjea-Matthes, A. Mukhopadhyay, A. BitMansour, I. L. Weissman, J. M. Y. Brown, and S. Strober Stepwise Development of Committed Progenitors in the Bone Marrow That Generate Functional T Cells in the Absence of the Thymus J. Immunol., October 1, 2005; 175(7): 4363 - 4373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Benz and C. C. Bleul A multipotent precursor in the thymus maps to the branching point of the T versus B lineage decision J. Exp. Med., July 5, 2005; 202(1): 21 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Lefebvre, M. C. Haks, M. O. Carleton, M. Rhodes, G. Sinnathamby, M. C. Simon, L. C. Eisenlohr, L. A. Garrett-Sinha, and D. L. Wiest Enforced Expression of Spi-B Reverses T Lineage Commitment and Blocks {beta}-Selection J. Immunol., May 15, 2005; 174(10): 6184 - 6194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Ferrell, S. T. Dorsam, H. Ohta, R. K. Humphries, M. K. Derynck, C. Haqq, C. Largman, and H. J. Lawrence Activation of Stem-Cell Specific Genes by HOXA9 and HOXA10 Homeodomain Proteins in CD34+ Human Cord Blood Cells Stem Cells, May 1, 2005; 23(5): 644 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Okuno, G. Huang, F. Rosenbauer, E. K. Evans, H. S. Radomska, H. Iwasaki, K. Akashi, F. Moreau-Gachelin, Y. Li, P. Zhang, et al. Potential Autoregulation of Transcription Factor PU.1 by an Upstream Regulatory Element Mol. Cell. Biol., April 1, 2005; 25(7): 2832 - 2845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhong, A. Takeda, R. Nazari, H. Shio, G. Blobel, and N. R. Yaseen Carrier-independent Nuclear Import of the Transcription Factor PU.1 via RanGTP-stimulated Binding to Nup153 J. Biol. Chem., March 18, 2005; 280(11): 10675 - 10682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Szutorisz, C. Canzonetta, A. Georgiou, C.-M. Chow, L. Tora, and N. Dillon Formation of an Active Tissue-Specific Chromatin Domain Initiated by Epigenetic Marking at the Embryonic Stem Cell Stage Mol. Cell. Biol., March 1, 2005; 25(5): 1804 - 1820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Puthier, F. Joly, M. Irla, M. Saade, G. Victorero, B. Loriod, and C. Nguyen A General Survey of Thymocyte Differentiation by Transcriptional Analysis of Knockout Mouse Models J. Immunol., November 15, 2004; 173(10): 6109 - 6118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Yui and E. V. Rothenberg Deranged Early T Cell Development in Immunodeficient Strains of Nonobese Diabetic Mice J. Immunol., November 1, 2004; 173(9): 5381 - 5391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. E. Mick, T. K. Starr, T. M. McCaughtry, L. K. McNeil, and K. A. Hogquist The Regulated Expression of a Diverse Set of Genes during Thymocyte Positive Selection In Vivo J. Immunol., November 1, 2004; 173(9): 5434 - 5444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Tiacci, S. Pileri, A. Orleth, R. Pacini, A. Tabarrini, F. Frenguelli, A. Liso, D. Diverio, F. Lo-Coco, and B. Falini PAX5 Expression in Acute Leukemias: Higher B-Lineage Specificity Than CD79a and Selective Association with t(8;21)-Acute Myelogenous Leukemia Cancer Res., October 15, 2004; 64(20): 7399 - 7404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hoflinger, K. Kesavan, M. Fuxa, C. Hutter, B. Heavey, F. Radtke, and M. Busslinger Analysis of Notch1 Function by In Vitro T Cell Differentiation of Pax5 Mutant Lymphoid Progenitors J. Immunol., September 15, 2004; 173(6): 3935 - 3944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Schmitt, M. Ciofani, H. T. Petrie, and J. C. Zuniga-Pflucker Maintenance of T Cell Specification and Differentiation Requires Recurrent Notch Receptor-Ligand Interactions J. Exp. Med., August 16, 2004; 200(4): 469 - 479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Arman, J. Calvo, M. E. Trojanowska, P. N. Cockerill, M. Santana, M. Lopez-Cabrera, J. Vives, and F. Lozano Transcriptional Regulation of Human CD5: Important Role of Ets Transcription Factors in CD5 Expression in T Cells J. Immunol., June 15, 2004; 172(12): 7519 - 7529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Anderson, R. Pant, A. L. Miracle, X. Sun, C. A. Luer, C. J. Walsh, J. C. Telfer, G. W. Litman, and E. V. Rothenberg Evolutionary Origins of Lymphocytes: Ensembles of T Cell and B Cell Transcriptional Regulators in a Cartilaginous Fish J. Immunol., May 15, 2004; 172(10): 5851 - 5860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Back, A. Dierich, C. Bronn, P. Kastner, and S. Chan PU.1 determines the self-renewal capacity of erythroid progenitor cells Blood, May 15, 2004; 103(10): 3615 - 3623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Harrow, J. U. Amuta, S. R. Hutchinson, F. Akwaa, and B. D. Ortiz Factors Binding a Non-classical Cis-element Prevent Heterochromatin Effects on Locus Control Region Activity J. Biol. Chem., April 23, 2004; 279(17): 17842 - 17849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Telfer, E. E. Hedblom, M. K. Anderson, M. N. Laurent, and E. V. Rothenberg Localization of the Domains in Runx Transcription Factors Required for the Repression of CD4 in Thymocytes J. Immunol., April 1, 2004; 172(7): 4359 - 4370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-P. Zhong, J. S. Maltzman, E. A. Hainey, and G. A. Koretzky Transcriptional Regulation of Src Homology 2 Domain-Containing Leukocyte Phosphoprotein of 76 kDa: Dissection of Key Promoter Elements J. Immunol., December 15, 2003; 171(12): 6621 - 6629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. D. Lieto, F. Borrego, C.-h. You, and J. E. Coligan Human CD94 Gene Expression: Dual Promoters Differing in Responsiveness to IL-2 or IL-15 J. Immunol., November 15, 2003; 171(10): 5277 - 5286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Throm and M. J. Klemsz PU.1 regulates glutathione peroxidase expression in neutrophils J. Leukoc. Biol., July 1, 2003; 74(1): 111 - 117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zaldumbide, F. Carlotti, P. Pognonec, and K. E. Boulukos The Role of the Ets2 Transcription Factor in the Proliferation, Maturation, and Survival of Mouse Thymocytes J. Immunol., November 1, 2002; 169(9): 4873 - 4881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Hadri, A. Ozog, F. Soncin, and A.-M. Lompre Basal Transcription of the Mouse Sarco(endo)plasmic Reticulum Ca2+-ATPase Type 3 Gene in Endothelial Cells Is Controlled by Ets-1 and Sp1 J. Biol. Chem., September 20, 2002; 277(39): 36471 - 36478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Reisdorff, A. En-Nia, I. Stefanidis, J. Floege, D. H. Lovett, and P. R. Mertens Transcription Factor Ets-1 Regulates Gelatinase A Gene Expression in Mesangial Cells J. Am. Soc. Nephrol., June 1, 2002; 13(6): 1568 - 1578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Ortiz, F. Harrow, D. Cado, B. Santoso, and A. Winoto Function and Factor Interactions of a Locus Control Region Element in the Mouse T Cell Receptor-{alpha}/Dad1 Gene Locus J. Immunol., October 1, 2001; 167(7): 3836 - 3845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Colucci, S. I. Samson, R. P. DeKoter, O. Lantz, H. Singh, and J. P. Di Santo Differential requirement for the transcription factor PU.1 in the generation of natural killer cells versus B and T cells Blood, May 1, 2001; 97(9): 2625 - 2632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Garrett-Sinha, R. Dahl, S. Rao, K. P. Barton, and M. C. Simon PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1 Blood, May 1, 2001; 97(9): 2908 - 2912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Chen, L. Rowen, L. Hood, and E. V. Rothenberg Differential Transcriptional Regulation of Individual TCR V{{beta}} Segments Before Gene Rearrangement J. Immunol., February 1, 2001; 166(3): 1771 - 1780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Carvajal and R. Sen Functional Analysis of the Murine TCR{beta}-Chain Gene Enhancer J. Immunol., June 15, 2000; 164(12): 6332 - 6339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Goetz, T.-L. Gu, N. A. Speck, and B. J. Graves Auto-Inhibition of Ets-1 Is Counteracted by DNA Binding Cooperativity with Core-Binding Factor alpha 2 Mol. Cell. Biol., January 1, 2000; 20(1): 81 - 90. [Abstract] [Full Text]
|
|
|
|
|
|
M. K. Anderson, X. Sun, A. L. Miracle, G. W. Litman, and E. V. Rothenberg Evolution of hematopoiesis: Three members of the PU.1 transcription factor family in a cartilaginous fish, Raja eglanteria PNAS, January 16, 2001; 98(2): 553 - 558. [Abstract] [Full Text] [PDF]
|
|
