spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kofron, M.
Right arrow Articles by Heasman, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kofron, M.
Right arrow Articles by Heasman, J.

Development, Vol 126, Issue 24 5759-5770, Copyright © 1999 by Company of Biologists

JOURNAL ARTICLES

Mesoderm induction in Xenopus is a zygotic event regulated by maternal VegT via TGFbeta growth factors

M Kofron, T Demel, J Xanthos, J Lohr, B Sun, H Sive, S Osada, C Wright, C Wylie and J Heasman
Department of Genetics, Cell Biology and Development, University of Minnesota, 321 Church Street SE, Minneapolis, MN 55455, USA.

The maternal transcription factor VegT is important for establishing the primary germ layers in Xenopus. In previous work, we showed that the vegetal masses of embryos lacking maternal VegT do not produce mesoderm-inducing signals and that mesoderm formation in these embryos occurred ectopically, from the vegetal area rather than the equatorial zone of the blastula. Here we have increased the efficiency of the depletion of maternal VegT mRNA and have studied the effects on mesoderm formation. We find that maternal VegT is required for the formation of 90% of mesodermal tissue, as measured by the expression of mesodermal markers MyoD, cardiac actin, Xbra, Xwnt8 and alphaT4 globin. Furthermore, the transcription of FGFs and TGFbetas, Xnr1, Xnr2, Xnr4 and derriere does not occur in VegT-depleted embryos. We test whether these growth factors may be endogenous factors in mesoderm induction, by studying their ability to rescue the phenotype of VegT-depleted embryos, when their expression is restricted to the vegetal mass. We find that Xnr1, Xnr2, Xnr4 and derriere mRNA all rescue mesoderm formation, as well as the formation of blastopores and the wild-type body axis. Derriere rescues trunk and tail while nr1, nr2 and nr4 rescue head, trunk and tail. We conclude that mesoderm induction in Xenopus depends on a maternal transcription factor regulating these zygotic growth factors.


This article has been cited by other articles:


Home page
 Genes Dev.Home page
J. Souopgui, B. Rust,, J. Vanhomwegen, J. Heasman, K. A. Henningfeld, E. Bellefroid, and T. Pieler
The RNA-binding protein XSeb4R: a positive regulator of VegT mRNA stability and translation that is required for germ layer formation in Xenopus
Genes & Dev., September 1, 2008; 22(17): 2347 - 2352.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Range, F. Lapraz, M. Quirin, S. Marro, L. Besnardeau, and T. Lepage
Cis-regulatory analysis of nodal and maternal control of dorsal-ventral axis formation by Univin, a TGF-{beta} related to Vg1
Development, October 15, 2007; 134(20): 3649 - 3664.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. B. Steiner, M. J. Engleka, Q. Lu, E. C. Piwarzyk, S. Yaklichkin, J. L. Lefebvre, J. W. Walters, L. Pineda-Salgado, P. A. Labosky, and D. S. Kessler
FoxD3 regulation of Nodal in the Spemann organizer is essential for Xenopus dorsal mesoderm development
Development, December 15, 2006; 133(24): 4827 - 4838.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
J.-S. Wang, Y.-L. Yang, C.-J. Wu, K. J. Ouyang, K.-Y. Tseng, C.-G. Chen, H. Wang, and H.-J. Lo
The DNA-binding domain of CaNdt80p is required to activate CDR1 involved in drug resistance in Candida albicans.
J. Med. Microbiol., October 1, 2006; 55(Pt 10): 1403 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. L. Zamparini, T. Watts, C. E. Gardner, S. R. Tomlinson, G. I. Johnston, and J. M. Brickman
Hex acts with {beta}-catenin to regulate anteroposterior patterning via a Groucho-related co-repressor and Nodal
Development, September 15, 2006; 133(18): 3709 - 3722.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. Sinner, P. Kirilenko, S. Rankin, E. Wei, L. Howard, M. Kofron, J. Heasman, H. R. Woodland, and A. M. Zorn
Global analysis of the transcriptional network controlling Xenopus endoderm formation.
Development, May 1, 2006; 133(10): 1955 - 1966.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Heasman
Patterning the early Xenopus embryo.
Development, April 1, 2006; 133(7): 1205 - 1217.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. Birsoy, M. Kofron, K. Schaible, C. Wylie, and J. Heasman
Vg1 is an essential signaling molecule in Xenopus development
Development, January 1, 2006; 133(1): 15 - 20.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. W. Houston and C. Wylie
Maternal Xenopus Zic2 negatively regulates Nodal-related gene expression during anteroposterior patterning
Development, November 1, 2005; 132(21): 4845 - 4855.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Collart, K. Verschueren, A. Rana, J. C. Smith, and D. Huylebroeck
The novel Smad-interacting protein Smicl regulates Chordin expression in the Xenopus embryo
Development, October 15, 2005; 132(20): 4575 - 4586.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Q. Tao, B. Lloyd, S. Lang, D. Houston, A. Zorn, and C. Wylie
A novel G protein-coupled receptor, related to GPR4, is required for assembly of the cortical actin skeleton in early Xenopus embryos
Development, June 15, 2005; 132(12): 2825 - 2836.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. A. Afouda, A. Ciau-Uitz, and R. Patient
GATA4, 5 and 6 mediate TGF{beta} maintenance of endodermal gene expression in Xenopus embryos
Development, February 15, 2005; 132(4): 763 - 774.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. Birsoy, L. Berg, P. H. Williams, J. C. Smith, C. C. Wylie, J. L. Christian, and J. Heasman
XPACE4 is a localized pro-protein convertase required for mesoderm induction and the cleavage of specific TGF{beta} proteins in Xenopus development
Development, February 1, 2005; 132(3): 591 - 602.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
C.-G. Chen, Y.-L. Yang, H.-I Shih, C.-L. Su, and H.-J. Lo
CaNdt80 Is Involved in Drug Resistance in Candida albicans by Regulating CDR1
Antimicrob. Agents Chemother., December 1, 2004; 48(12): 4505 - 4512.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Daniels, K. Shimizu, A. M. Zorn, and S.-i. Ohnuma
Negative regulation of Smad2 by PIASy is required for proper Xenopus mesoderm formation
Development, November 15, 2004; 131(22): 5613 - 5626.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Kofron, H. Puck, H. Standley, C. Wylie, R. Old, M. Whitman, and J. Heasman
New roles for FoxH1 in patterning the early embryo
Development, October 15, 2004; 131(20): 5065 - 5078.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
O. Piepenburg, D. Grimmer, P. H. Williams, and J. C. Smith
Activin redux: specification of mesodermal pattern in Xenopus by graded concentrations of endogenous activin B
Development, October 15, 2004; 131(20): 4977 - 4986.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
F. C. Wardle and J. C. Smith
Refinement of gene expression patterns in the early Xenopus embryo
Development, October 1, 2004; 131(19): 4687 - 4696.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. Sinner, S. Rankin, M. Lee, and A. M. Zorn
Sox17 and {beta}-catenin cooperate to regulate the transcription of endodermal genes
Development, July 1, 2004; 131(13): 3069 - 3080.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Kofron, C. Wylie, and J. Heasman
The role of Mixer in patterning the early Xenopus embryo
Development, May 15, 2004; 131(10): 2431 - 2441.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Pocock, J. Ahringer, M. Mitsch, S. Maxwell, and A. Woollard
A regulatory network of T-box genes and the even-skipped homologue vab-7 controls patterning and morphogenesis in C. elegans
Development, May 15, 2004; 131(10): 2373 - 2385.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. Katsumoto, T. Arikawa, J.-y. Doi, H. Fujii, S.-i. Nishimatsu, and M. Sakai
Cytoplasmic and molecular reconstruction of Xenopus embryos: synergy of dorsalizing and endo-mesodermalizing determinants drives early axial patterning
Development, March 1, 2004; 131(5): 1135 - 1144.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Mathieu, K. Griffin, P. Herbomel, T. Dickmeis, U. Strahle, D. Kimelman, F. M. Rosa, and N. Peyrieras
Nodal and Fgf pathways interact through a positive regulatory loop and synergize to maintain mesodermal cell populations
Development, February 1, 2004; 131(3): 629 - 641.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Z. A. Khan, M. Cukiernik, J. R. Gonder, and S. Chakrabarti
Oncofetal Fibronectin in Diabetic Retinopathy
Invest. Ophthalmol. Vis. Sci., January 1, 2004; 45(1): 287 - 295.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. E. E. Bruce, C. Howley, Y. Zhou, S. L. Vickers, L. M. Silver, M. L. King, and R. K. Ho
The maternally expressed zebrafish T-box gene eomesodermin regulates organizer formation
Development, November 15, 2003; 130(22): 5503 - 5517.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Trindade, N. Messenger, C. Papin, D. Grimmer, L. Fairclough, M. Tada, and J. C. Smith
Regulation of apoptosis in theXenopus embryo by Bix3
Development, October 1, 2003; 130(19): 4611 - 4622.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. W. Houston and C. Wylie
The Xenopus LIM-homeodomain protein Xlim5 regulates the differential adhesion properties of early ectoderm cells
Development, June 15, 2003; 130(12): 2695 - 2704.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Yokota, M. Kofron, M. Zuck, D. W. Houston, H. Isaacs, M. Asashima, C. C. Wylie, and J. Heasman
A novel role for a nodal-related protein; Xnr3 regulates convergent extension movements via the FGF receptor
Development, May 15, 2003; 130(10): 2199 - 2212.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. L. Amacher, B. W. Draper, B. R. Summers, and C. B. Kimmel
The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate
Development, March 9, 2003; 129(14): 3311 - 3323.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. E. Fisher, H. V. Isaacs, and M. E. Pownall
eFGF is required for activation of XmyoD expression in the myogenic cell lineage of Xenopus laevis
Development, March 5, 2003; 129(6): 1307 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. W. Houston, M. Kofron, E. Resnik, R. Langland, O. Destree, C. Wylie, and J. Heasman
Repression of organizer genes in dorsal and ventral Xenopus cells mediated by maternal XTcf3
Development, September 1, 2002; 129(17): 4015 - 4025.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. B. Xanthos, M. Kofron, Q. Tao, K. Schaible, C. Wylie, and J. Heasman
The roles of three signaling pathways in the formation and function of the Spemann Organizer
Development, September 1, 2002; 129(17): 4027 - 4043.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Y. Belenkaya, C. Han, H. J. Standley, X. Lin, D. W. Houston, J. Heasman, and X. Lin
pygopus encodes a nuclear protein essential for Wingless/Wnt signaling
Development, September 1, 2002; 129(17): 4089 - 4101.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. Kofron, J. Heasman, S. A. Lang, and C. C. Wylie
Plakoglobin is required for maintenance of the cortical actin skeleton in early Xenopus embryos and for cdc42-mediated wound healing
J. Cell Biol., August 19, 2002; 158(4): 695 - 708.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
D. Y.R. Stainier
A glimpse into the molecular entrails of endoderm formation
Genes & Dev., April 15, 2002; 16(8): 893 - 907.
[Full Text] [PDF]

Home page
 Genes Dev.Home page
C. Ring, S. Ogata, L. Meek, J. Song, T. Ohta, K. Miyazono, and K. W.Y. Cho
The role of a Williams-Beuren syndrome-associated helix-loop-helix domain-containing transcription factor in activin/nodal signaling
Genes & Dev., April 1, 2002; 16(7): 820 - 835.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Miyanaga, I. Torregroza, and T. Evans
A Maternal Smad Protein Regulates Early Embryonic Apoptosis in Xenopus laevis
Mol. Cell. Biol., March 1, 2002; 22(5): 1317 - 1328.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P. M. Eimon and R. M. Harland
Effects of heterodimerization and proteolytic processing on Derriere and Nodal activity: implications for mesoderm induction in Xenopus
Development, January 7, 2002; 129(13): 3089 - 3103.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. A. Vokes and P. A. Krieg
Endoderm is required for vascular endothelial tube formation, but not for angioblast specification
Development, January 2, 2002; 129(3): 775 - 785.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Schohl and F. Fagotto
{beta}-catenin, MAPK and Smad signaling during early Xenopus development
Development, January 1, 2002; 129(1): 37 - 52.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. F. Wu, H. Nakamura, A. P.-Y. Chan, Y.-H. Zhou, T. Cao, J. Kuang, S.-G. Gong, G. He, and L. D. Etkin
Tumorhead, a Xenopus gene product that inhibits neural differentiation through regulation of proliferation
Development, September 1, 2001; 128(17): 3381 - 3393.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. B. Shappell, R. A. Gupta, S. Manning, R. Whitehead, W. E. Boeglin, C. Schneider, T. Case, J. Price, G. S. Jack, T. M. Wheeler, et al.
15S-Hydroxyeicosatetraenoic Acid Activates Peroxisome Proliferator-activated Receptor {{gamma}} and Inhibits Proliferation in PC3 Prostate Carcinoma Cells
Cancer Res., January 1, 2001; 61(2): 497 - 503.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
J. Xanthos, M Kofron, C Wylie, and J Heasman
Maternal VegT is the initiator of a molecular network specifying endoderm in Xenopus laevis
Development, January 1, 2001; 128(2): 167 - 180.
[Abstract] [PDF]

Home page
 DevelopmentHome page
O. Wessely and E. M. De Robertis
The Xenopus homologue of Bicaudal-C is a localized maternal mRNA that can induce endoderm formation
Development, May 15, 2000; 127(10): 2053 - 2062.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
C. H. Ezal, C. D. Marion, and W. C. Smith
Primary Structure Requirements for Xenopus Nodal-related 3 and a Comparison with Regions Required by Xenopus Nodal-related 2
J. Biol. Chem., May 5, 2000; 275(19): 14124 - 14131.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S Takahashi, C Yokota, K Takano, K Tanegashima, Y Onuma, J Goto, and M Asashima
Two novel nodal-related genes initiate early inductive events in Xenopus Nieuwkoop center
Development, January 12, 2000; 127(24): 5319 - 5329.
[Abstract] [PDF]

Home page
 DevelopmentHome page
H Weber, C. Symes, M. Walmsley, A. Rodaway, and R. Patient
A role for GATA5 in Xenopus endoderm specification
Development, January 10, 2000; 127(20): 4345 - 4360.
[Abstract] [PDF]

Home page
 DevelopmentHome page
S Faure, M. Lee, T Keller, P ten Dijke, and M Whitman
Endogenous patterns of TGFbeta superfamily signaling during early Xenopus development
Development, January 7, 2000; 127(13): 2917 - 2931.
[Abstract] [PDF]

Home page
 DevelopmentHome page
K. Tremblay, P. Hoodless, E. Bikoff, and E. Robertson
Formation of the definitive endoderm in mouse is a Smad2-dependent process
Development, January 7, 2000; 127(14): 3079 - 3090.
[Abstract] [PDF]

Home page
 DevelopmentHome page
S. Osada, Y Saijoh, A Frisch, C. Yeo, H Adachi, M Watanabe, M Whitman, H Hamada, and C. Wright
Activin/nodal responsiveness and asymmetric expression of a Xenopus nodal-related gene converge on a FAST-regulated module in intron 1
Development, January 6, 2000; 127(11): 2503 - 2514.
[Abstract] [PDF]

Home page
 DevelopmentHome page
R Vignali, L Poggi, F Madeddu, and G Barsacchi
HNF1(beta) is required for mesoderm induction in the Xenopus embryo
Development, January 4, 2000; 127(7): 1455 - 1465.
[Abstract] [PDF]

Home page
 DevelopmentHome page
E Agius, M Oelgeschlager, O Wessely, C Kemp, and E. De Robertis
Endodermal Nodal-related signals and mesoderm induction in Xenopus
Development, January 3, 2000; 127(6): 1173 - 1183.
[Abstract] [PDF]

Home page
 DevelopmentHome page
C. Hyde and R. Old
Regulation of the early expression of the Xenopus nodal-related 1 gene, Xnr1
Development, January 3, 2000; 127(6): 1221 - 1229.
[Abstract] [PDF]


© The Company of Biologists Ltd 1999