|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 127, Issue 1 197-207, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
I Siddiqi, G Ganesh, U Grossniklaus and V Subbiah
Centre for Cellular and Molecular Biology, Hyderabad, India. imran@ccmb.ap.nic.in
In higher plants the gametophyte consists of a gamete in association with a small number of haploid cells, specialized for sexual reproduction. The female gametophyte or embryo sac, is contained within the ovule and develops from a single cell, the megaspore which is formed by meiosis of the megaspore mother cell. The dyad mutant of Arabidopsis, described herein, represents a novel class among female sterile mutants in plants. dyad ovules contain two large cells in place of an embryo sac. The two cells represent the products of a single division of the megaspore mother cell followed by an arrest in further development of the megaspore. We addressed the question of whether the division of the megaspore mother cell in the mutant was meiotic or mitotic by examining the expression of two markers that are normally expressed in the megaspore mother cell during meiosis. Our observations indicate that in dyad, the megaspore mother cell enters but fails to complete meiosis, arresting at the end of meiosis 1 in the majority of ovules. This was corroborated by a direct observation of chromosome segregation during division of the megaspore mother cell, showing that the division is a reductional and not an equational one. In a minority of dyad ovules, the megaspore mother cell does not divide. Pollen development and male fertility in the mutant is normal, as is the rest of the ovule that surrounds the female gametophyte. The embryo sac is also shown to have an influence on the nucellus in wild type. The dyad mutation therefore specifically affects a function that is required in the female germ cell precursor for meiosis. The identification and analysis of mutants specifically affecting female meiosis is an initial step in understanding the molecular mechanisms underlying early events in the pathway of female reproductive development.
This article has been cited by other articles:
|
|
 |
|
  K. A. Boateng, X. Yang, F. Dong, H. A. Owen, and C. A. Makaroff SWI1 Is Required for Meiotic Chromosome Remodeling Events Mol Plant, July 1, 2008; 1(4): 620 - 633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Liu and L.-J. Qu Meiotic and Mitotic Cell Cycle Mutants Involved in Gametophyte Development in Arabidopsis Mol Plant, July 1, 2008; 1(4): 564 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Portereiko, A. Lloyd, J. G. Steffen, J. A. Punwani, D. Otsuga, and G. N. Drews AGL80 Is Required for Central Cell and Endosperm Development in Arabidopsis PLANT CELL, August 1, 2006; 18(8): 1862 - 1872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kaur, J. Sebastian, and I. Siddiqi The Arabidopsis-mei2-Like Genes Play a Role in Meiosis and Vegetative Growth in Arabidopsis PLANT CELL, March 1, 2006; 18(3): 545 - 559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. A Wilson and C. Yang Plant gametogenesis: conservation and contrasts in development Reproduction, November 1, 2004; 128(5): 483 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Acosta-Garcia and J.-P. Vielle-Calzada A Classical Arabinogalactan Protein Is Essential for the Initiation of Female Gametogenesis in Arabidopsis PLANT CELL, October 1, 2004; 16(10): 2614 - 2628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. van Dijk and J. M. T. Bakx-Schotman Formation of Unreduced Megaspores (Diplospory) in Apomictic Dandelions (Taraxacum officinale, s.l.) Is Controlled by a Sex-Specific Dominant Locus Genetics, January 1, 2004; 166(1): 483 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. V. Reddy, J. Kaur, B. Agashe, V. Sundaresan, and I. Siddiqi The DUET gene is necessary for chromosome organization and progression during male meiosis in Arabidopsis and encodes a PHD finger protein Development, December 15, 2003; 130(24): 5975 - 5987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S.A. Al-Hammadi, Y. Sreelakshmi, S. Negi, I. Siddiqi, and R. Sharma The polycotyledon Mutant of Tomato Shows Enhanced Polar Auxin Transport Plant Physiology, September 1, 2003; 133(1): 113 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Yang, C. A. Makaroff, and H. Ma The Arabidopsis MALE MEIOCYTE DEATH1 Gene Encodes a PHD-Finger Protein That Is Required for Male Meiosis PLANT CELL, June 1, 2003; 15(6): 1281 - 1295. [Abstract] [Full Text]
|
|
|
|
|
|
B. Agashe, C. K. Prasad, and I. Siddiqi Identification and analysis of DYAD: a gene required for meiotic chromosome organisation and female meiotic progression in Arabidopsis Development, March 10, 2003; 129(16): 3935 - 3943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Caryl, G. H. Jones, and F. C. H. Franklin Dissecting plant meiosis using Arabidopsis thaliana mutants J. Exp. Bot., January 1, 2003; 54(380): 25 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Gro{beta}-Hardt, M. Lenhard, and T. Laux WUSCHEL signaling functions in interregional communication during Arabidopsis ovule development Genes & Dev., May 1, 2002; 16(9): 1129 - 1138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Magnard, M. Yang, Y.-C. S. Chen, M. Leary, and S. McCormick The Arabidopsis Gene Tardy Asynchronous Meiosis Is Required for the Normal Pace and Synchrony of Cell Division during Male Meiosis Plant Physiology, November 1, 2001; 127(3): 1157 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V Raz, J. Bergervoet, and M Koornneef Sequential steps for developmental arrest in Arabidopsis seeds Development, January 1, 2001; 128(2): 243 - 252. [Abstract] [PDF]
|
|
