|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 127, Issue 10 2089-2098, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
A Majumdar, K Lun, M Brand and IA Drummond
Renal Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA.
Pax genes are important developmental regulators and function at multiple stages of vertebrate kidney organogenesis. In this report, we have used the zebrafish pax2.1 mutant no isthmus to investigate the role for pax2.1 in development of the pronephros. We demonstrate a requirement for pax2.1 in multiple aspects of pronephric development including tubule and duct epithelial differentiation and cloaca morphogenesis. Morphological analysis demonstrates that noi(- )larvae specifically lack pronephric tubules while glomerular cell differentiation is unaffected. In addition, pax2.1 expression in the lateral cells of the pronephric primordium is required to restrict the domains of Wilms' tumor suppressor (wt1) and vascular endothelial growth factor (VEGF) gene expression to medial podocyte progenitors. Ectopic podocyte-specific marker expression in pronephric duct cells correlates with loss of expression of the pronephric tubule and duct-specific markers mAb 3G8 and a Na(+)/K(+) ATPase (&agr;)1 subunit. The results suggest that the failure in pronephric tubule differentiation in noi arises from a patterning defect during differentiation of the pronephric primordium and that mutually inhibitory regulatory interactions play an important role in defining the boundary between glomerular and tubule progenitors in the forming nephron.
This article has been cited by other articles:
|
|
 |
|
  S. P. Mudumana, D. Hentschel, Y. Liu, A. Vasilyev, and I. A. Drummond odd skipped related1 reveals a novel role for endoderm in regulating kidney versus vascular cell fate Development, October 15, 2008; 135(20): 3355 - 3367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. J. Murawski, D. B. Myburgh, J. Favor, and I. R. Gupta Vesico-ureteric reflux and urinary tract development in the Pax21Neu+/ mouse Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1736 - F1745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, N. Pathak, A. Kramer-Zucker, and I. A. Drummond Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros Development, March 15, 2007; 134(6): 1111 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhao, Z. Yang, J. K. Phelan, D. A. Wheeler, S. Lin, and E. R. B. McCabe Zebrafish dax1 Is Required for Development of the Interrenal Organ, the Adrenal Cortex Equivalent Mol. Endocrinol., November 1, 2006; 20(11): 2630 - 2640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Gibert, A. Gajewski, A. Meyer, and G. Begemann Induction and prepatterning of the zebrafish pectoral fin bud requires axial retinoic acid signaling Development, July 15, 2006; 133(14): 2649 - 2659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. E. Shmukler, C. E. Kurschat, G. E. Ackermann, L. Jiang, Y. Zhou, B. Barut, A. K. Stuart-Tilley, J. Zhao, L. I. Zon, I. A. Drummond, et al. Zebrafish slc4a2/ae2 anion exchanger: cDNA cloning, mapping, functional characterization, and localization Am J Physiol Renal Physiol, October 1, 2005; 289(4): F835 - F849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Igarashi Overview: Nonmammalian Organisms for Studies of Kidney Development and Disease J. Am. Soc. Nephrol., February 1, 2005; 16(2): 296 - 298. [Full Text] [PDF]
|
|
|
|
|
|
D. Ribes, E. Fischer, A. Calmont, and J. Rossert Transcriptional Control of Epithelial Differentiation during Kidney Development J. Am. Soc. Nephrol., June 1, 2003; 14(90001): S9 - 15. [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Spitsbergen and M. L. Kent The State of the Art of the Zebrafish Model for Toxicology and Toxicologic Pathology Research--Advantages and Current Limitations Toxicol Pathol, January 1, 2003; 31(1_suppl): 62 - 87. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Bouchard, A. Souabni, M. Mandler, A. Neubuser, and M. Busslinger Nephric lineage specification by Pax2 and Pax8 Genes & Dev., November 15, 2002; 16(22): 2958 - 2970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wendl, K. Lun, M. Mione, J. Favor, M. Brand, S. W. Wilson, and K. B. Rohr pax2.1 is required for the development of thyroid follicles in zebrafish Development, August 1, 2002; 129(15): 3751 - 3760. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Briggs The zebrafish: a new model organism for integrative physiology Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2002; 282(1): R3 - R9. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Sun and N. Hopkins vhnf1, the MODY5 and familial GCKD-associated gene, regulates regional specification of the zebrafish gut, pronephros, and hindbrain Genes & Dev., December 1, 2001; 15(23): 3217 - 3229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. C. Serluca and M. C. Fishman Pre-pattern in the pronephric kidney field of zebrafish Development, June 15, 2001; 128(12): 2233 - 2241. [Abstract] [Full Text] [PDF]
|
|
