spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nguyen, A. V.
Right arrow Articles by Pollard, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nguyen, A. V.
Right arrow Articles by Pollard, J. W.

Development, Vol 127, Issue 14 3107-3118, Copyright © 2000 by Company of Biologists

JOURNAL ARTICLES

Transforming growth factor beta3 induces cell death during the first stage of mammary gland involution

AV Nguyen and JW Pollard
Department of Developmental and Molecular Biology, and Department of Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine, Bronx, New York, USA.

Involution of the mammary gland following weaning is divided into two distinct phases. Initially, milk stasis results in the induction of local factors that cause apoptosis in the alveolar epithelium. Secondly after a prolonged absence of suckling, the consequent decline in circulating lactogenic hormone concentrations initiates remodeling of the mammary gland to the virgin-like state. We have shown that immediately following weaning TGFbeta3 mRNA and protein is rapidly induced in the mammary epithelium and that this precedes the onset of apoptosis. Unilateral inhibition of suckling and hormonal reconstitution experiments showed that TGFbeta3 induction is regulated by milk stasis and not by the circulating hormonal concentration. Directed expression of TGFbeta3 in the alveolar epithelium of lactating mice using a beta-lactoglobulin promoter mobilized SMAD4 translocation to the nucleus and caused apoptosis of these cells, but not tissue remodeling. Transplantation of neonatal mammary tissue derived from TGFbeta3 null mutant mice into syngenic hosts resulted in a significant inhibition of cell death compared to wild-type mice upon milk stasis. These results provide direct evidence that TGFbeta3 is a local mammary factor induced by milk stasis that causes apoptosis in the mammary gland epithelium during involution.


This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
K. Singh, S. R. Davis, J. M. Dobson, A. J. Molenaar, T. T. Wheeler, C. G. Prosser, V. C. Farr, K. Oden, K. M. Swanson, C. V. C. Phyn, et al.
cDNA Microarray Analysis Reveals that Antioxidant and Immune Genes Are Upregulated During Involution of the Bovine Mammary Gland
J Dairy Sci, June 1, 2008; 91(6): 2236 - 2246.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Cocolakis, M. Dai, L. Drevet, J. Ho, E. Haines, S. Ali, and J.-J. Lebrun
Smad Signaling Antagonizes STAT5-mediated Gene Transcription and Mammary Epithelial Cell Differentiation
J. Biol. Chem., January 18, 2008; 283(3): 1293 - 1307.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Fischer, H. Stuckas, M. Gluth, T. D. Russell, M. C. Rudolph, N. E. Beeman, S. Bachmann, S. Umemura, Y. Ohashi, M. C. Neville, et al.
Impaired tight junction sealing and precocious involution in mammary glands of PKN1 transgenic mice
J. Cell Sci., July 1, 2007; 120(13): 2272 - 2283.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
R Serra and M R Crowley
Mouse models of transforming growth factor {beta} impact in breast development and cancer
Endocr. Relat. Cancer, December 1, 2005; 12(4): 749 - 760.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Thangaraju, M. Rudelius, B. Bierie, M. Raffeld, S. Sharan, L. Hennighausen, A-M. Huang, and E. Sterneck
C/EBP{delta} is a crucial regulator of pro-apoptotic gene expression during mammary gland involution
Development, November 1, 2005; 132(21): 4675 - 4685.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. M. Blakely, L. Sintasath, C. M. D'Cruz, K. T. Hahn, K. D. Dugan, G. K. Belka, and L. A. Chodosh
Developmental stage determines the effects of MYC in the mammary epithelium
Development, March 1, 2005; 132(5): 1147 - 1160.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. A. Green, M. J. Naylor, E. T. Lowe, P. Wang, E. Marshman, and C. H. Streuli
Caspase-mediated Cleavage of Insulin Receptor Substrate
J. Biol. Chem., June 11, 2004; 279(24): 25149 - 25156.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. P. Bailey, K. M. Nieport, M. P. Herbst, S. Srivastava, R. A. Serra, and N. D. Horseman
Prolactin and Transforming Growth Factor-{beta} Signaling Exert Opposing Effects on Mammary Gland Morphogenesis, Involution, and the Akt-Forkhead Pathway
Mol. Endocrinol., May 1, 2004; 18(5): 1171 - 1184.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. G. Kim, H.-S. Jong, T.-Y. Kim, J. W. Lee, N. K. Kim, S. H. Hong, and Y.-J. Bang
Transforming Growth Factor-{beta}1 Induces Apoptosis through Fas Ligand-independent Activation of the Fas Death Pathway in Human Gastric SNU-620 Carcinoma Cells
Mol. Biol. Cell, February 1, 2004; 15(2): 420 - 434.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Cuervo and L. Covarrubias
Death is the major fate of medial edge epithelial cells and the cause of basal lamina degradation during palatogenesis
Development, January 1, 2004; 131(1): 15 - 24.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
W. Li, W. Qiao, L. Chen, X. Xu, X. Yang, D. Li, C. Li, S. G. Brodie, M. M. Meguid, L. Hennighausen, et al.
Squamous cell carcinoma and mammary abscess formation through squamous metaplasia in Smad4/Dpc4 conditional knockout mice
Development, December 15, 2003; 130(24): 6143 - 6153.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. E. Gorska, R. A. Jensen, Y. Shyr, M. E. Aakre, N. A. Bhowmick, and H. L. Moses
Transgenic Mice Expressing a Dominant-Negative Mutant Type II Transforming Growth Factor-{beta} Receptor Exhibit Impaired Mammary Development and Enhanced Mammary Tumor Formation
Am. J. Pathol., October 1, 2003; 163(4): 1539 - 1549.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
E. A. Kritikou, A. Sharkey, K. Abell, P. J. Came, E. Anderson, R. W. E. Clarkson, and C. J. Watson
A dual, non-redundant, role for LIF as a regulator of development and STAT3-mediated cell death in mammary gland
Development, August 1, 2003; 130(15): 3459 - 3468.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. S. Jeruss, C. D. Sturgis, A. W. Rademaker, and T. K. Woodruff
Down-Regulation of Activin, Activin Receptors, and Smads in High-Grade Breast Cancer
Cancer Res., July 1, 2003; 63(13): 3783 - 3790.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
I. Vetharaniam, S. R. Davis, T. K. Soboleva, P. R. Shorten, and G. C. Wake
Modeling the Interaction of Milking Frequency and Nutrition on Mammary Gland Growth and Lactation
J Dairy Sci, June 1, 2003; 86(6): 1987 - 1996.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. U. Mysorekar, R. G. Lorenz, and J. I. Gordon
A Gnotobiotic Transgenic Mouse Model for Studying Interactions between Small Intestinal Enterocytes and Intraepithelial Lymphocytes
J. Biol. Chem., September 27, 2002; 277(40): 37811 - 37819.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
C. M. D'Cruz, S. E. Moody, S. R. Master, J. L. Hartman, E. A. Keiper, M. B. Imielinski, J. D. Cox, J. Y. Wang, S. I. Ha, B. A. Keister, et al.
Persistent Parity-Induced Changes in Growth Factors, TGF-{beta}3, and Differentiation in the Rodent Mammary Gland
Mol. Endocrinol., September 1, 2002; 16(9): 2034 - 2051.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
K. B. Ewan, G. Shyamala, S. A. Ravani, Y. Tang, R. Akhurst, L. Wakefield, and M. H. Barcellos-Hoff
Latent Transforming Growth Factor-{beta} Activation in Mammary Gland : Regulation by Ovarian Hormones Affects Ductal and Alveolar Proliferation
Am. J. Pathol., June 1, 2002; 160(6): 2081 - 2093.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
B. S. Wiseman and Z. Werb
Stromal Effects on Mammary Gland Development and Breast Cancer
Science, May 10, 2002; 296(5570): 1046 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 Cell Growth Differ.Home page
Y.-a. Yang, B. Tang, G. Robinson, L. Hennighausen, S. G. Brodie, C.-X. Deng, and L. M. Wakefield
Smad3 in the Mammary Epithelium Has a Nonredundant Role in the Induction of Apoptosis, but not in the Regulation of Proliferation or Differentiation by Transforming Growth Factor-{beta}
Cell Growth Differ., March 1, 2002; 13(3): 123 - 130.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C.-R. Chen, Y. Kang, and J. Massague
Inaugural Article: Defective repression of c-myc in breast cancer cells: A loss at the core of the transforming growth factor beta growth arrest program
PNAS, January 30, 2001; 98(3): 992 - 999.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. L. Segev, Y. Hoshiya, A. E. Stephen, M. Hoshiya, T. T. Tran, D. T. MacLaughlin, P. K. Donahoe, and S. Maheswaran
Mullerian Inhibiting Substance Regulates NFkappa B Signaling and Growth of Mammary Epithelial Cells in Vivo
J. Biol. Chem., July 13, 2001; 276(29): 26799 - 26806.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2000