|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Development, Vol 127, Issue 16 3407-3417, Copyright © 2000 by Company of Biologists
JOURNAL ARTICLES |
L Saude, K Woolley, P Martin, W Driever and DL Stemple
Division of Developmental Biology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
We have investigated axis-inducing activities and cellular fates of the zebrafish organizer using a new method of transplantation that allows the transfer of both deep and superficial organizer tissues. Previous studies have demonstrated that the zebrafish embryonic shield possesses classically defined dorsal organizer activity. When we remove the morphologically defined embryonic shield, embryos recover and are completely normal by 24 hours post-fertilization. We find that removal of the morphological shield does not remove all goosecoid- and floating head-expressing cells, suggesting that the morphological shield does not comprise the entire organizer region. Complete removal of the embryonic shield and adjacent marginal tissue, however, leads to a loss of both prechordal plate and notochord. In addition, these embryos are cyclopean, show a significant loss of floor plate and primary motorneurons and display disrupted somite patterning. Motivated by apparent discrepancies in the literature we sought to test the axis-inducing activity of the embryonic shield. A previous study suggested that the shield is capable of only partial axis induction, specifically being unable to induce the most anterior neural tissues. Contrary to this study, we find shields can induce complete secondary axes when transplanted into host ventral germ-ring. In induced secondary axes donor tissue contributes to notochord, prechordal plate and floor plate. When explanted shields are divided into deep and superficial fragments and separately transplanted we find that deep tissue is able to induce the formation of ectopic axes with heads but lacking posterior tissues. We conclude that the deep tissue included in our transplants is important for proper head formation.
This article has been cited by other articles:
|
|
 |
|
  R. Esterberg, J.-M. Delalande, and A. Fritz Tailbud-derived Bmp4 drives proliferation and inhibits maturation of zebrafish chordamesoderm Development, December 1, 2008; 135(23): 3891 - 3901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chang and R. M. Harland Neural induction requires continued suppression of both Smad1 and Smad2 signals during gastrulation Development, November 1, 2007; 134(21): 3861 - 3872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Reim and M. Brand Maternal control of vertebrate dorsoventral axis formation and epiboly by the POU domain protein Spg/Pou2/Oct4 Development, July 15, 2006; 133(14): 2757 - 2770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Burns, D. Traver, E. Mayhall, J. L. Shepard, and L. I. Zon Hematopoietic stem cell fate is established by the Notch-Runx pathway Genes & Dev., October 1, 2005; 19(19): 2331 - 2342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Stemple Structure and function of the notochord: an essential organ for chordate development Development, June 1, 2005; 132(11): 2503 - 2512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rhinn, K. Lun, M. Luz, M. Werner, and M. Brand Positioning of the midbrain-hindbrain boundary organizer through global posteriorization of the neuroectoderm mediated by Wnt8 signaling Development, March 15, 2005; 132(6): 1261 - 1272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Zoltewicz, N. J. Stewart, R. Leung, and A. S. Peterson Atrophin 2 recruits histone deacetylase and is required for the function of multiple signaling centers during mouse embryogenesis Development, January 1, 2004; 131(1): 3 - 14. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Parsons, S. M. Pollard, L. Saude, B. Feldman, P. Coutinho, E. M. A. Hirst, and D. L. Stemple Zebrafish mutants identify an essential role for laminins in notochord formation Development, January 7, 2002; 129(13): 3137 - 3146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Erter, T. P. Wilm, N. Basler, C. V. E. Wright, and L. Solnica-Krezel Wnt8 is required in lateral mesendodermal precursors for neural posteriorization in vivo Development, September 15, 2001; 128(18): 3571 - 3583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-P. Heisenberg, C. Houart, M. Take-uchi, G.-J. Rauch, N. Young, P. Coutinho, I. Masai, L. Caneparo, M. L. Concha, R. Geisler, et al. A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon Genes & Dev., June 1, 2001; 15(11): 1427 - 1434. [Abstract] [Full Text] [PDF]
|
|
