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Development 128, 3485-3495 (2001)
© 2001 The Company of Biologists Limited

Hedgehog signaling is required for primary motoneuron induction in zebrafish

Katharine E. Lewis and Judith S. Eisen*

Institute of Neuroscience, 1254 University of Oregon, Eugene, OR 97403, USA

*Author for correspondence (e-mail: eisen{at}uoneuro.uoregon.edu)

Accepted June 29, 2001

Sonic hedgehog (Shh) is crucial for motoneuron development in chick and mouse. However, zebrafish embryos homozygous for a deletion of the shh locus have normal numbers of motoneurons, raising the possibility that zebrafish motoneurons may be specified differently. Unlike other vertebrates, zebrafish express three hh genes in the embryonic midline: shh, echidna hedgehog (ehh) and tiggywinkle hedgehog (twhh). Therefore, it is possible that Twhh and Ehh are sufficient for motoneuron formation in the absence of Shh. To test this hypothesis we have eliminated, or severely reduced, all three Hh signals using mutations that directly or indirectly reduce Hh signaling and antisense morpholinos. Our analysis shows that Hh signals are required for zebrafish motoneuron induction. However, each of the three zebrafish Hhs is individually dispensable for motoneuron development because the other two can compensate for its loss. Our results also suggest that Twhh and Shh are more important for motoneuron development than Ehh.

Key words: Hh, Smoothened, Motoneuron, syu, smu, cyc, flh, shh, ehh, twhh, Morpholino, Ventral neural tube, Floor plate, Spinal cord patterning




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© The Company of Biologists Ltd 2001