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Development, Vol 128, Issue 2 253-261, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
B Jungblut and RJ Sommer
Max-Planck Institut fur Entwicklungsbiologie, Abt. Evolutionsbiologie, Spemannstrasse 37-39, Tubingen, Germany.
In free-living nematodes, developmental processes like the formation of the vulva, can be studied at a cellular level. Cell lineage and ablation studies have been carried out in various nematode species and multiple changes in vulval patterning have been identified. In Pristionchus pacificus, vulva formation differs from Caenorhabditis elegans with respect to several autonomous and conditional aspects of cell fate specification. To understand the molecular basis of these evolutionary changes, we have performed a genetic analysis of vulva formation in P. pacificus. Here, we describe two mutants where the vulva is shifted posteriorly, affecting which precursor cells will form vulval tissue in P. pacificus. Mutant animals show a concomitant posterior displacement of the gonadal anchor cell, indicating that the gonad and the vulva are affected in a similar way. We show that mutations in the even-skipped homolog of nematodes, vab-7, cause these posterior displacements. In addition, cell ablation studies in the vab-7 mutant indicate that the altered position of the gonad not only changes the cell fate pattern but also the developmental competence of vulval precursor cells. Investigation of Cel-vab-7 mutant animals showed a similar but weaker vulva defective phenotype to the one described for Ppa-vab-7.
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  B. Yi and R. J. Sommer The pax-3 gene is involved in vulva formation in Pristionchus pacificus and is a target of the Hox gene lin-39 Development, September 1, 2007; 134(17): 3111 - 3119. [Abstract] [Full Text] [PDF]
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 S. Louvet-Vallee, I. Kolotuev, B. Podbilewicz, and M.-A. Felix Control of Vulval Competence and Centering in the Nematode Oscheius sp. 1 CEW1 Genetics, January 1, 2003; 163(1): 133 - 146. [Abstract] [Full Text] [PDF]
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J. Srinivasan, W. Sinz, C. Lanz, A. Brand, R. Nandakumar, G. Raddatz, H. Witte, H. Keller, I. Kipping, A. Pires-daSilva, et al. A Bacterial Artificial Chromosome-Based Genetic Linkage Map of the Nematode Pristionchus pacificus Genetics, September 1, 2002; 162(1): 129 - 134. [Abstract] [Full Text] [PDF]
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B. Jungblut, A. Pires-daSilva, and R. J. Sommer Formation of the egg-laying system in Pristionchus pacificus requires complex interactions between gonadal, mesodermal and epidermal tissues and does not rely on single cell inductions Development, September 15, 2001; 128(18): 3395 - 3404. [Abstract] [Full Text] [PDF]
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