QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Artero, R. D. Articles by Baylies, M. K. Search for Related Content PubMed PubMed Citation Articles by Artero, R. D. Articles by Baylies, M. K.

The immunoglobulin-like protein Hibris functions as a dose-dependent regulator of myoblast fusion and is differentially controlled by Ras and Notch signaling

Molecular Biology Program, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA

*Author for correspondence (e-mail: m-baylies{at}ski.mskcc.org)

Accepted August 14, 2001

Hibris (Hbs) is a transmembrane immunoglobulin-like protein that shows extensive homology to Drosophila Sticks and stones (Sns) and human kidney protein Nephrin. Hbs is expressed in embryonic visceral, somatic and pharyngeal mesoderm among other tissues. In the somatic mesoderm, Hbs is restricted to fusion competent myoblasts and is regulated by Notch and Ras signaling pathways. Embryos that lack or overexpress hbs show a partial block of myoblast fusion, followed by abnormal muscle morphogenesis. Abnormalities in visceral mesoderm are also observed. In vivo mapping of functional domains suggests that the intracellular domain mediates Hbs activity. Hbs and its paralog, Sns, co-localize at the cell membrane of fusion-competent myoblasts. The two proteins act antagonistically: loss of sns dominantly suppresses the hbs myoblast fusion and visceral mesoderm phenotypes, and enhances Hbs overexpression phenotypes. Data from a P-homed enhancer reporter into hbs and co-localization studies with Sns suggest that hbs is not continuously expressed in all fusion-competent myoblasts during the fusion process. We propose that the temporal pattern of hbs expression within fusion-competent myoblasts may reflect previously undescribed functional differences within this myoblast population.

Key words: Myoblast fusion, Mesoderm, Morphogenesis, Sticks and stones, Hbs, Drosophila

This article has been cited by other articles:

				A. M. Waters, M. Y.J. Wu, T. Onay, J. Scutaru, J. Liu, C. G. Lobe, S. E. Quaggin, and T. D. Piscione Ectopic Notch Activation in Developing Podocytes Causes Glomerulosclerosis J. Am. Soc. Nephrol., June 1, 2008; 19(6): 1139 - 1157. [Full Text] [PDF]

				K. S. Kocherlakota, J.-m. Wu, J. McDermott, and S. M. Abmayr Analysis of the Cell Adhesion Molecule Sticks-and-Stones Reveals Multiple Redundant Functional Domains, Protein-Interaction Motifs and Phosphorylated Tyrosines That Direct Myoblast Fusion in Drosophila melanogaster Genetics, March 1, 2008; 178(3): 1371 - 1383. [Abstract] [Full Text] [PDF]

				B. E. Richardson, K. Beckett, S. J. Nowak, and M. K. Baylies SCAR/WAVE and Arp2/3 are crucial for cytoskeletal remodeling at the site of myoblast fusion Development, December 15, 2007; 134(24): 4357 - 4367. [Abstract] [Full Text] [PDF]

				P. Aaltonen and H. Holthofer The nephrin-based slit diaphragm: new insight into the signalling platform identifies targets for therapy Nephrol. Dial. Transplant., December 1, 2007; 22(12): 3408 - 3410. [Full Text] [PDF]

				H. Duan and H. T. Nguyen Distinct Posttranscriptional Mechanisms Regulate the Activity of the Zn Finger Transcription Factor Lame duck during Drosophila Myogenesis Mol. Cell. Biol., February 15, 2006; 26(4): 1414 - 1423. [Abstract] [Full Text] [PDF]

				S. D. Menon, Z. Osman, K. Chenchill, and W. Chia A positive feedback loop between Dumbfounded and Rolling pebbles leads to myotube enlargement in Drosophila J. Cell Biol., June 20, 2005; 169(6): 909 - 920. [Abstract] [Full Text] [PDF]

				E. H. Chen and E. N. Olson Unveiling the Mechanisms of Cell-Cell Fusion Science, April 15, 2005; 308(5720): 369 - 373. [Abstract] [Full Text] [PDF]

				T. Benzing Signaling at the Slit Diaphragm J. Am. Soc. Nephrol., June 1, 2004; 15(6): 1382 - 1391. [Full Text] [PDF]

				L. M. Escudero, S.-Y. Wei, W.-H. Chiu, J. Modolell, and J.-C. Hsu Echinoid synergizes with the Notch signaling pathway in Drosophila mesothorax bristle patterning Development, December 22, 2003; 130(25): 6305 - 6316. [Abstract] [Full Text] [PDF]

				R. Artero, E. E. Furlong, K. Beckett, M. P. Scott, and M. Baylies Notch and Ras signaling pathway effector genes expressed in fusion competent and founder cells during Drosophila myogenesis Development, December 22, 2003; 130(25): 6257 - 6272. [Abstract] [Full Text] [PDF]

				P. J. Solano, B. Mugat, D. Martin, F. Girard, J.-M. Huibant, C. Ferraz, B. Jacq, J. Demaille, and F. Maschat Genome-wide identification of in vivo Drosophila Engrailed-binding DNA fragments and related target genes Development, April 1, 2003; 130(7): 1243 - 1254. [Abstract] [Full Text] [PDF]

				P. Mundel and S. J. Shankland Podocyte Biology and Response to Injury J. Am. Soc. Nephrol., December 1, 2002; 13(12): 3005 - 3015. [Full Text] [PDF]

				M. R. Pollak Inherited Podocytopathies: FSGS and Nephrotic Syndrome from a Genetic Viewpoint J. Am. Soc. Nephrol., December 1, 2002; 13(12): 3016 - 3023. [Full Text] [PDF]

				J. Reiser, G. von Gersdorff, M. Simons, K. Schwarz, C. Faul, L. Giardino, T. Heider, M. Loos, and P. Mundel Novel concepts in understanding and management of glomerular proteinuria Nephrol. Dial. Transplant., June 1, 2002; 17(6): 951 - 955. [Full Text] [PDF]