|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Skirball Institute of Biomolecular Medicine and Department of Pharmacology, New York University Medical School, 540 First Avenue, New York, NY 10016, USA
*Author for correspondence (e-mail: sink{at}saturn.med.nyu.edu)
Accepted August 9, 2001
Hibris encodes a protein that is a newly identified member of the immunoglobulin superfamily and has homology to vertebrate Nephrins and Drosophila Sticks-and-Stones. The Hibris protein has eight Ig-like domains, a fibronectin domain and a 160 amino acid cytoplasmic tail. The hibris transcript is expressed in a broad range of tissues and across life stages. In the embryo, hibris transcript is present in the mesectoderm, then in a group of cells at the developing CNS midline and in a subset of glia. In the periphery, hibris is expressed by fusion competent myoblasts and the epidermal muscle attachment site cells. Deletion analyses show that loss of hibris does not visibly affect embryonic CNS or somatic muscle development. However overexpressing hibris in the somatic mesoderm disrupts myoblast fusion. Furthermore, when overexpressed in the epidermis, Hibris causes comprehensive derangement of muscle insertion locations. A similar myoblast fusion defect is observed when the Drosophila homologs of DM-GRASP/BEN/SC1 (irregular chiasm-roughest and dumbfounded) are deleted together. Our S2 cell aggregation assays have revealed a heterotypic interaction between Hibris and Dumbfounded, but not between Hibris and Irregular Chiasm-Roughest. We propose that Hibris is an extracellular partner for Dumbfounded and potentially mediates the response of myoblasts to this attractant.
Key words: Drosophila, hibris, Nephrin, Mesoderm, Myoblast, Myotube, Muscle attachment sites, Mesectoderm, Dumbfounded, DM-GRASP, BEN, SC1
This article has been cited by other articles:
|
|
 |
|
  P. Garg, R. Verma, D. Nihalani, D. B. Johnstone, and L. B. Holzman Neph1 Cooperates with Nephrin To Transduce a Signal That Induces Actin Polymerization Mol. Cell. Biol., December 15, 2007; 27(24): 8698 - 8712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. E. Richardson, K. Beckett, S. J. Nowak, and M. K. Baylies SCAR/WAVE and Arp2/3 are crucial for cytoskeletal remodeling at the site of myoblast fusion Development, December 15, 2007; 134(24): 4357 - 4367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Aaltonen and H. Holthofer The nephrin-based slit diaphragm: new insight into the signalling platform identifies targets for therapy Nephrol. Dial. Transplant., December 1, 2007; 22(12): 3408 - 3410. [Full Text] [PDF]
|
|
|
|
 |
|
 R. J.M. Coward, G. I. Welsh, A. Koziell, S. Hussain, R. Lennon, L. Ni, J. M. Tavare, P. W. Mathieson, and M. A. Saleem Nephrin Is Critical for the Action of Insulin on Human Glomerular Podocytes Diabetes, April 1, 2007; 56(4): 1127 - 1135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Duan and H. T. Nguyen Distinct Posttranscriptional Mechanisms Regulate the Activity of the Zn Finger Transcription Factor Lame duck during Drosophila Myogenesis Mol. Cell. Biol., February 15, 2006; 26(4): 1414 - 1423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Menon, Z. Osman, K. Chenchill, and W. Chia A positive feedback loop between Dumbfounded and Rolling pebbles leads to myotube enlargement in Drosophila J. Cell Biol., June 20, 2005; 169(6): 909 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Gerke, L. Sellin, O. Kretz, D. Petraschka, H. Zentgraf, T. Benzing, and G. Walz NEPH2 Is Located at the Glomerular Slit Diaphragm, Interacts with Nephrin and Is Cleaved from Podocytes by Metalloproteinases J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1693 - 1702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. H. Chen and E. N. Olson Unveiling the Mechanisms of Cell-Cell Fusion Science, April 15, 2005; 308(5720): 369 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Soler, M. Daczewska, J. P. Da Ponte, B. Dastugue, and K. Jagla Coordinated development of muscles and tendons of the Drosophila leg Development, December 15, 2004; 131(24): 6041 - 6051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Benzing Signaling at the Slit Diaphragm J. Am. Soc. Nephrol., June 1, 2004; 15(6): 1382 - 1391. [Full Text] [PDF]
|
|
|
|
|
|
R. Artero, E. E. Furlong, K. Beckett, M. P. Scott, and M. Baylies Notch and Ras signaling pathway effector genes expressed in fusion competent and founder cells during Drosophila myogenesis Development, December 22, 2003; 130(25): 6257 - 6272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-M. Barletta, I. A. Kovari, R. K. Verma, D. Kerjaschki, and L. B. Holzman Nephrin and Neph1 Co-localize at the Podocyte Foot Process Intercellular Junction and Form cis Hetero-oligomers J. Biol. Chem., May 23, 2003; 278(21): 19266 - 19271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Gerke, T. B. Huber, L. Sellin, T. Benzing, and G. Walz Homodimerization and Heterodimerization of the Glomerular Podocyte Proteins Nephrin and NEPH1 J. Am. Soc. Nephrol., April 1, 2003; 14(4): 918 - 926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Solano, B. Mugat, D. Martin, F. Girard, J.-M. Huibant, C. Ferraz, B. Jacq, J. Demaille, and F. Maschat Genome-wide identification of in vivo Drosophila Engrailed-binding DNA fragments and related target genes Development, April 1, 2003; 130(7): 1243 - 1254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mundel and S. J. Shankland Podocyte Biology and Response to Injury J. Am. Soc. Nephrol., December 1, 2002; 13(12): 3005 - 3015. [Full Text] [PDF]
|
|
|
|
|
|
M. R. Pollak Inherited Podocytopathies: FSGS and Nephrotic Syndrome from a Genetic Viewpoint J. Am. Soc. Nephrol., December 1, 2002; 13(12): 3016 - 3023. [Full Text] [PDF]
|
|
|
|
|
|
J. Reiser, G. von Gersdorff, M. Simons, K. Schwarz, C. Faul, L. Giardino, T. Heider, M. Loos, and P. Mundel Novel concepts in understanding and management of glomerular proteinuria Nephrol. Dial. Transplant., June 1, 2002; 17(6): 951 - 955. [Full Text] [PDF]
|
|
