QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Giorgini, F. Articles by Braun, R. E. Search for Related Content PubMed PubMed Citation Articles by Giorgini, F. Articles by Braun, R. E.

Translational repression by MSY4 inhibits spermatid differentiation in mice

Flaviano Giorgini, Holly G. Davies^* and Robert E. Braun

Department of Genome Sciences, University of Washington, Seattle, WA, USA
^* Present address: National Institutes of Health (NIH), National Institute of Diabetes and Digestive Kidney Disorders (NIDDK), Laboratory of Cellular and Developmental Biology (LCDB), 50 South Drive, MSC-8028, Bethesda, MD 20892-8028, USA

Author for correspondence (e-mail: braun{at}washington.edu)

Accepted 24 April 2002

In developing male germ cells, newly synthesized protamine mRNAs are stored for up to 7 days before translational activation. Translational repression of protamine 1 (Prm1) mRNA requires sequences present in its 3' untranslated region (UTR) and substantial evidence suggests a role for the murine Y-box protein MSY4 in this process. To determine if MSY4 can mediate translational repression in vivo, we generated transgenic mice in which the temporal window of MSY4 expression was extended during spermatogenesis. Expression of MSY4 disrupted the normal completion of spermatogenesis and caused dominant sterility. Immunocytochemical analysis of several markers, including the protamines, indicated that MSY4 prevented normal activation of translation. mRNAs whose translation was inhibited contained at least one MSY4 RNA recognition site, suggesting sequence-dependent translational repression. Altered translational activation resulted in defective processing of protamine 2 and severe defects in sperm morphogenesis. These results suggest that MSY4 plays an active role in translational repression of several mRNAs in differentiating spermatids.

Key words: Translational control, mRNPs, Protamine, Spermatogenesis, RNA-binding, Mouse

This article has been cited by other articles:

				C. C. Baker and M. T. Fuller Translational control of meiotic cell cycle progression and spermatid differentiation in male germ cells by a novel eIF4G homolog Development, August 1, 2007; 134(15): 2863 - 2869. [Abstract] [Full Text] [PDF]

				C. Ravel, S. Chantot-Bastaraud, B. El Houate, I. Berthaut, L. Verstraete, V. De Larouziere, D. Lourenco, A. Dumaine, J.M. Antoine, J. Mandelbaum, et al. Mutations in the protamine 1 gene associated with male infertility Mol. Hum. Reprod., July 1, 2007; 13(7): 461 - 464. [Abstract] [Full Text] [PDF]

				E. Aivatiadou, E. Mattei, M. Ceriani, L. Tilia, and G. Berruti Impaired Fertility and Spermiogenetic Disorders with Loss of Cell Adhesion in Male Mice Expressing an Interfering Rap1 Mutant Mol. Biol. Cell, April 1, 2007; 18(4): 1530 - 1542. [Abstract] [Full Text] [PDF]

				Z. H. Lu, J. T. Books, and T. J. Ley Cold Shock Domain Family Members YB-1 and MSY4 Share Essential Functions during Murine Embryogenesis Mol. Cell. Biol., November 15, 2006; 26(22): 8410 - 8417. [Abstract] [Full Text] [PDF]

				Z. H. Lu, J. T. Books, and T. J. Ley YB-1 Is Important for Late-Stage Embryonic Development, Optimal Cellular Stress Responses, and the Prevention of Premature Senescence Mol. Cell. Biol., June 1, 2005; 25(11): 4625 - 4637. [Abstract] [Full Text] [PDF]

				J. Yang, S. Medvedev, J. Yu, L. C. Tang, J. E. Agno, M. M. Matzuk, R. M. Schultz, and N. B. Hecht Absence of the DNA-/RNA-binding protein MSY2 results in male and female infertility PNAS, April 19, 2005; 102(16): 5755 - 5760. [Abstract] [Full Text] [PDF]

				J. Yang, S. Medvedev, P. P. Reddi, R. M. Schultz, and N. B. Hecht The DNA/RNA-binding protein MSY2 marks specific transcripts for cytoplasmic storage in mouse male germ cells PNAS, February 1, 2005; 102(5): 1513 - 1518. [Abstract] [Full Text] [PDF]

				C. R. Shirley, S. Hayashi, S. Mounsey, R. Yanagimachi, and M. L. Meistrich Abnormalities and Reduced Reproductive Potential of Sperm from Tnp1- and Tnp2-Null Double Mutant Mice Biol Reprod, October 1, 2004; 71(4): 1220 - 1229. [Abstract] [Full Text] [PDF]

				H.-J. Shi, A. Z. Wu, M. Santos, Z.-M. Feng, L. Huang, Y.-M. Chen, K. Zhu, and C.-L. C. Chen Cloning and Characterization of Rat Spermatid Protein SSP411: A Thioredoxin-Like Protein J Androl, July 1, 2004; 25(4): 479 - 493. [Abstract] [Full Text] [PDF]

				J. E. Shima, D. J. McLean, J. R. McCarrey, and M. D. Griswold The Murine Testicular Transcriptome: Characterizing Gene Expression in the Testis During the Progression of Spermatogenesis Biol Reprod, July 1, 2004; 71(1): 319 - 330. [Abstract] [Full Text] [PDF]

				R. W. Holdcraft and R. E. Braun Androgen receptor function is required in Sertoli cells for the terminal differentiation of haploid spermatids Development, January 15, 2004; 131(2): 459 - 467. [Abstract] [Full Text] [PDF]

				V. Chennathukuzhi, C. R. Morales, M. El-Alfy, and N. B. Hecht The kinesin KIF17b and RNA-binding protein TB-RBP transport specific cAMP-responsive element modulator-regulated mRNAs in male germ cells PNAS, December 23, 2003; 100(26): 15566 - 15571. [Abstract] [Full Text] [PDF]

				C. Cho, H. Jung-Ha, W. D. Willis, E. H. Goulding, P. Stein, Z. Xu, R. M. Schultz, N. B. Hecht, and E. M. Eddy Protamine 2 Deficiency Leads to Sperm DNA Damage and Embryo Death in Mice Biol Reprod, July 1, 2003; 69(1): 211 - 217. [Abstract] [Full Text] [PDF]