QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bagri, A. Articles by Pleasure, S. J. Search for Related Content PubMed PubMed Citation Articles by Bagri, A. Articles by Pleasure, S. J.

The chemokine SDF1 regulates migration of dentate granule cells

Anil Bagri^1,2, Theresa Gurney², Xiaoping He¹, Yong-Rui Zou^3,, Dan R. Littman³, Marc Tessier-Lavigne^2,*, and Samuel J. Pleasure^1,

¹ Neurodevelopmental Disorders Laboratory, Department of Neurology, Program in Neuroscience, University of California, San Francisco, CA 94143-0435, USA
² Departments of Anatomy and of Biochemistry and Biophyics, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143-0452, USA
³ Departments of Pathology and Microbiology, Skirball Institute of Biomolecular Medicine, Howard Hughes Medical Institute, New York University Medical Center, New York, NY 10016, USA
^* Present address: Department of Biological Sciences, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
Present address: Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA

Authors for correspondence (e-mail: samuelp{at}itsa.ucsf.edu and marctl{at}stanford.edu)

Accepted 27 June 2002

The dentate gyrus is the primary afferent pathway into the hippocampus, but there is little information concerning the molecular influences that govern its formation. In particular, the control of migration and cell positioning of dentate granule cells is not clear. We have characterized more fully the timing and route of granule cell migration during embryogenesis using in utero retroviral injections. Using this information, we developed an in vitro assay that faithfully recapitulates important events in dentate gyrus morphogenesis. In searching for candidate ligands that may regulate dentate granule cell migration, we found that SDF1, a chemokine that regulates cerebellar and leukocyte migration, and its receptor CXCR4 are expressed in patterns that suggest a role in dentate granule cell migration. Furthermore, CXCR4 mutant mice have a defect in granule cell position. Ectopic expression of SDF1 in our explant assay showed that it directly regulates dentate granule cell migration. Our study shows that a chemokine is necessary for the normal development of the dentate gyrus, a forebrain structure crucial for learning and memory.

Key words: Hippocampus, Neuronal migration, Chemokine, Dentate granule cell, Mouse

This article has been cited by other articles:

				C. D. Pozniak and S. J. Pleasure A Tale of Two Signals: Wnt and Hedgehog in Dentate Neurogenesis Sci. Signal., January 24, 2006; 2006(319): pe5 - pe5. [Abstract] [Full Text] [PDF]

				S. H. Chalasani, K. A. Sabelko, M. J. Sunshine, D. R. Littman, and J. A. Raper A Chemokine, SDF-1, Reduces the Effectiveness of Multiple Axonal Repellents and Is Required for Normal Axon Pathfinding J. Neurosci., February 15, 2003; 23(4): 1360 - 1371. [Abstract] [Full Text] [PDF]