|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
doi: 10.1242/10.1242/dev.00515
1 Molecular Medicine Program, Ottawa Health Research Institute, 501 Smyth Road,
Ottawa, ON K1H 8L6, Canada
2 Department of Molecular and Cellular Biology, The Biolabs, Harvard University,
16 Divinity Avenue, Cambridge, MA 02138, USA
3 Eye Institute, Center for Neuromuscular Disease, and Department of
Biochemistry, Microbiology and Immunology, University of Ottawa, 451 Smyth
Road, Ottawa, ON K1H 8M5, Canada
* Author for correspondence (e-mail: vwallace{at}ohri.ca)
Accepted 31 March 2003
The development of optic stalk neuroepithelial cells depends on Hedgehog (Hh) signaling, yet the source(s) of Hh protein in the optic stalk is unknown. We provide genetic evidence that sonic hedgehog (Shh) from retinal ganglion cells (RGCs) promotes the development of optic disc and stalk neuroepithelial cells. We demonstrate that RGCs express Shh soon after differentiation, and cells at the optic disc in close proximity to the Shh-expressing RGCs upregulate Hh target genes, which suggests they are responding to RGC-derived Shh signaling. Conditional ablation of Shh in RGCs caused a complete loss of optic disc astrocyte precursor cells, resulting in defective axon guidance in the retina, as well as conversion of the neuroepithelial cells in the optic stalk to pigmented cells. We further show that Shh signaling modulates the size of the Pax2+ astrocyte precursor cell population at the optic disc in vitro. Together, these data provide a novel insight into the source of Hh that promotes neuroepithelial cell development in the mammalian optic disc and stalk.
Key words: Retinal ganglion cells, Shh, Ihh, Pax2, Optic disc/stalk, Neuroepithelial cells, Astrocyte precursor cells, Development, Mouse
This article has been cited by other articles:
|
|
 |
|
  C. Sanchez-Camacho and P. Bovolenta Autonomous and non-autonomous Shh signalling mediate the in vivo growth and guidance of mouse retinal ganglion cell axons Development, November 1, 2008; 135(21): 3531 - 3541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Liu and J. Nathans An essential role for frizzled 5 in mammalian ocular development Development, November 1, 2008; 135(21): 3567 - 3576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Pan, C. Carbe, A. Powers, E. E. Zhang, J. D. Esko, K. Grobe, G.-S. Feng, and X. Zhang Bud specific N-sulfation of heparan sulfate regulates Shp2-dependent FGF signaling during lacrimal gland induction Development, January 15, 2008; 135(2): 301 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Murakami, D. Umetsu, Y. Maeyama, M. Sato, S. Yoshida, and T. Tabata Focal adhesion kinase controls morphogenesis of the Drosophila optic stalk Development, April 15, 2007; 134(8): 1539 - 1548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Harada, C. Harada, and L. F. Parada Molecular regulation of visual system development: more than meets the eye Genes & Dev., February 15, 2007; 21(4): 367 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Pan, A. Woodbury, J. D. Esko, K. Grobe, and X. Zhang Heparan sulfate biosynthetic gene Ndst1 is required for FGF signaling in early lens development Development, December 15, 2006; 133(24): 4933 - 4944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Liu, S. Thurig, O. Mohamed, D. Dufort, and V. A. Wallace Mapping Canonical Wnt Signaling in the Developing and Adult Retina Invest. Ophthalmol. Vis. Sci., November 1, 2006; 47(11): 5088 - 5097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Morcillo, J. R. Martinez-Morales, F. Trousse, Y. Fermin, J. C. Sowden, and P. Bovolenta Proper patterning of the optic fissure requires the sequential activity of BMP7 and SHH Development, August 15, 2006; 133(16): 3179 - 3190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Koide, T. Hayata, and K. W. Y. Cho Negative regulation of Hedgehog signaling by the cholesterogenic enzyme 7-dehydrocholesterol reductase Development, June 15, 2006; 133(12): 2395 - 2405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, G. D. Dakubo, S. Thurig, C. J. Mazerolle, and V. A. Wallace Retinal ganglion cell-derived sonic hedgehog locally controls proliferation and the timing of RGC development in the embryonic mouse retina Development, November 15, 2005; 132(22): 5103 - 5113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Close, B. Gumuscu, and T. A. Reh Retinal neurons regulate proliferation of postnatal progenitors and Muller glia in the rat retina via TGF{beta} signaling Development, July 1, 2005; 132(13): 3015 - 3026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. Kay, B. A. Link, and H. Baier Staggered cell-intrinsic timing of ath5 expression underlies the wave of ganglion cell neurogenesis in the zebrafish retina Development, June 1, 2005; 132(11): 2573 - 2585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. West, W. D. Richardson, and M. Fruttiger Stabilization of the retinal vascular network by reciprocal feedback between blood vessels and astrocytes Development, April 15, 2005; 132(8): 1855 - 1862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kolpak, J. Zhang, and Z.-Z. Bao Sonic Hedgehog Has a Dual Effect on the Growth of Retinal Ganglion Axons Depending on Its Concentration J. Neurosci., March 30, 2005; 25(13): 3432 - 3441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Li, K. Shirabe, C. Thisse, B. Thisse, H. Okamoto, I. Masai, and J. Y. Kuwada Chemokine Signaling Guides Axons within the Retina in Zebrafish J. Neurosci., February 16, 2005; 25(7): 1711 - 1717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pan, Z. Yang, L. Feng, and L. Gan Functional equivalence of Brn3 POU-domain transcription factors in mouse retinal neurogenesis Development, February 15, 2005; 132(4): 703 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Miyawaki, A. Uemura, M. Dezawa, R. T. Yu, C. Ide, S. Nishikawa, Y. Honda, Y. Tanabe, and T. Tanabe Tlx, an Orphan Nuclear Receptor, Regulates Cell Numbers and Astrocyte Development in the Developing Retina J. Neurosci., September 15, 2004; 24(37): 8124 - 8134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Spence, M. Madhavan, J. D. Ewing, D. K. Jones, B. M. Lehman, and K. Del Rio-Tsonis The hedgehog pathway is a modulator of retina regeneration Development, September 15, 2004; 131(18): 4607 - 4621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, Z. Jin, and Z.-Z. Bao Disruption of gradient expression of Zic3 resulted in abnormal intra-retinal axon projection Development, April 1, 2004; 131(7): 1553 - 1562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Mu, P. D. Beremand, S. Zhao, R. Pershad, H. Sun, A. Scarpa, S. Liang, T. L. Thomas, and W. H. Klein Discrete gene sets depend on POU domain transcription factor Brn3b/Brn-3.2/POU4f2 for their expression in the mouse embryonic retina Development, March 15, 2004; 131(6): 1197 - 1210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C.M. Black, C. J. Mazerolle, Y. Wang, K. D. Campsall, D. Petrin, B. C. Leonard, K. F. Damji, D. G. Evans, D. McLeod, and V. A. Wallace Abnormalities of the vitreoretinal interface caused by dysregulated Hedgehog signaling during retinal development Hum. Mol. Genet., December 15, 2003; 12(24): 3269 - 3276. [Abstract] [Full Text] [PDF]
|
|
