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doi: 10.1242/10.1242/dev.00561
1 Department of Molecular Biology, University of Wyoming, PO Box 3944, Laramie,
WY 82071-3944, USA
2 Howard Hughes Medical Institute and Department of Molecular, Cellular, and
Developmental Biology, University of Colorado, Boulder, CO 80309-0347,
USA
* Author for correspondence (e-mail: davidfay{at}uwyo.edu)
Accepted 24 April 2003
The retinoblastoma gene product has been implicated in the regulation of multiple cellular and developmental processes, including a well-defined role in the control of cell cycle progression. The Caenorhabditis elegans retinoblastoma protein homolog, LIN-35, is also a key regulator of cell cycle entry and, as shown by studies of synthetic multivulval genes, plays an important role in the determination of vulval cell fates. We demonstrate an additional and unexpected function for lin-35 in organ morphogenesis. Using a genetic approach to isolate lin-35 synthetic-lethal mutations, we have identified redundant roles for lin-35 and ubc-18, a gene that encodes an E2 ubiquitin-conjugating enzyme closely related to human UBCH7. lin-35 and ubc-18 cooperate to control one or more steps during pharyngeal morphogenesis. Based on genetic and phenotypic analyses, this role for lin-35 in pharyngeal morphogenesis appears to be distinct from its cell cycle-related functions. lin-35 and ubc-18 may act in concert to regulate the levels of one or more critical targets during C. elegans development.
Key words: lin-35, Retinoblastoma, ubc-18, Ubiquitin, C. elegans, Pharynx
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