|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online August 4, 2003
doi: 10.1242/10.1242/dev.00618
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
Author for correspondence (e-mail:
jacksond{at}cshl.org)
Accepted 20 May 2003
Intercellular communication delivers critical information for
position-dependent specification of cell fate. In plants, a novel mechanism
for cell-to-cell communication involves the intercellular trafficking of
regulatory proteins and mRNAs. The maize KNOTTED1 (KN1) gene
acts non cell-autonomously in the maize leaf, and KN1 was the first plant
protein shown to traffic cell-to-cell, presumably through plasmodesmata. We
have compared the intercellular trafficking of green fluorescent protein (GFP)
fusions of KN1 and Arabidopsis KN1-related homeobox proteins to that
of the viral movement protein from turnip vein clearing tobamovirus. We show
that there is specific developmental regulation of GFP
KN1 trafficking.
GFPKN1 was able to traffic from the inner layers of the leaf to the
epidermis, but not in the opposite direction, from epidermis to mesophyll.
However, GFP or the GFPmovement protein fusion moved readily out of the
epidermis. GFPKN1 was however able to traffic out of the epidermal (L1)
layer in the shoot apical meristem, indicating that KN1 movement out of the L1
was developmentally regulated. GFPKNAT1/BREVIPEDICELLUS and
GFPSHOOTMERISTEMLESS fusions could also traffic from the L1 to the L2/L3
layers of the meristem. In a test for the functional significance of
trafficking, we showed that L1-specific expression of KN1 or of KNAT1 was able
to partially complement the strong shootmeristemless-11
(stm-11) mutant. However, a cell-autonomous GUS fusion to KN1 showed
neither trafficking ability nor complementation of stm-11 when
expressed in the L1. These results suggest that the activity of KN1 and
related homeobox proteins is maintained following intercellular trafficking,
and that trafficking may be required for their normal developmental
function.
Key words: Homeodomain, KNOX, Shoot meristem, knotted1, GFP, Plasmodesmata, Protein trafficking, Arabidopsis thaliana
This article has been cited by other articles:
|
|
 |
|
  C. A. Cullis, B. J. Vorster, C. Van Der Vyver, and K. J. Kunert Transfer of Genetic Material Between the Chloroplast and Nucleus: How is it Related to Stress in Plants? Ann. Bot., September 18, 2008; (2008) mcn173v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Bolduc, S. Hake, and D. Jackson Dual Functions of the KNOTTED1 Homeodomain: Sequence-Specific DNA Binding and Regulation of Cell-to-Cell Transport Sci. Signal., June 10, 2008; 1(23): pe28 - pe28. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-W. Wang, R. Schwab, B. Czech, E. Mica, and D. Weigel Dual Effects of miR156-Targeted SPL Genes and CYP78A5/KLUH on Plastochron Length and Organ Size in Arabidopsis thaliana PLANT CELL, May 1, 2008; 20(5): 1231 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Winter, G. Kollwig, S. Zhang, and F. Kragler MPB2C, a Microtubule-Associated Protein, Regulates Non-Cell-Autonomy of the Homeodomain Protein KNOTTED1 PLANT CELL, October 1, 2007; 19(10): 3001 - 3018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kumar, K. Kushalappa, D. Godt, M. S. Pidkowich, S. Pastorelli, S. R. Hepworth, and G. W. Haughn The Arabidopsis BEL1-LIKE HOMEODOMAIN Proteins SAW1 and SAW2 Act Redundantly to Regulate KNOX Expression Spatially in Leaf Margins PLANT CELL, September 1, 2007; 19(9): 2719 - 2735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kessler, B. Townsley, and N. Sinha L1 Division and Differentiation Patterns Influence Shoot Apical Meristem Maintenance Plant Physiology, August 1, 2006; 141(4): 1349 - 1362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cole, C. Nolte, and W. Werr Nuclear import of the transcription factor SHOOT MERISTEMLESS depends on heterodimerization with BLH proteins expressed in discrete sub-domains of the shoot apical meristem of Arabidopsis thaliana Nucleic Acids Res., March 2, 2006; 34(4): 1281 - 1292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kurata, T. Ishida, C. Kawabata-Awai, M. Noguchi, S. Hattori, R. Sano, R. Nagasaka, R. Tominaga, Y. Koshino-Kimura, T. Kato, et al. Cell-to-cell movement of the CAPRICE protein in Arabidopsis root epidermal cell differentiation Development, December 15, 2005; 132(24): 5387 - 5398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. I. Prokhnevsky, V. V. Peremyslov, and V. V. Dolja Actin Cytoskeleton Is Involved in Targeting of a Viral Hsp70 Homolog to the Cell Periphery J. Virol., November 15, 2005; 79(22): 14421 - 14428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Lee, K.-i. Taoka, B.-C. Yoo, G. Ben-Nissan, D.-J. Kim, and W. J. Lucas Plasmodesmal-Associated Protein Kinase in Tobacco and Arabidopsis Recognizes a Subset of Non-Cell-Autonomous Proteins PLANT CELL, October 1, 2005; 17(10): 2817 - 2831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-H. Chen, G.-W. Tian, Y. Gafni, and V. Citovsky Effects of Calreticulin on Viral Cell-to-Cell Movement Plant Physiology, August 1, 2005; 138(4): 1866 - 1876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, A. Krichevsky, M. Vaidya, T. Tzfira, and V. Citovsky Uncoupling of the functions of the Arabidopsis VIP1 protein in transient and stable plant genetic transformation by Agrobacterium PNAS, April 19, 2005; 102(16): 5733 - 5738. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Kim, Y. Rim, J. Wang, and D. Jackson A novel cell-to-cell trafficking assay indicates that the KNOX homeodomain is necessary and sufficient for intercellular protein and mRNA trafficking Genes & Dev., April 1, 2005; 19(7): 788 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hackbusch, K. Richter, J. Muller, F. Salamini, and J. F. Uhrig A central role of Arabidopsis thaliana ovate family proteins in networking and subcellular localization of 3-aa loop extension homeodomain proteins PNAS, March 29, 2005; 102(13): 4908 - 4912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kim, E. Cho, K. Crawford, F. D. Hempel, and P. C. Zambryski Cell-to-cell movement of GFP during embryogenesis and early seedling development in Arabidopsis PNAS, February 8, 2005; 102(6): 2227 - 2231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Gallagher and P. N. Benfey Not just another hole in the wall: understanding intercellular protein trafficking Genes & Dev., January 15, 2005; 19(2): 189 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Champagne and N. Sinha Compound leaves: equal to the sum of their parts? Development, September 15, 2004; 131(18): 4401 - 4412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Kimbrough, R. Salinas-Mondragon, W. F. Boss, C. S. Brown, and H. W. Sederoff The Fast and Transient Transcriptional Network of Gravity and Mechanical Stimulation in the Arabidopsis Root Apex Plant Physiology, September 1, 2004; 136(1): 2790 - 2805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-C. Yoo, F. Kragler, E. Varkonyi-Gasic, V. Haywood, S. Archer-Evans, Y. M. Lee, T. J. Lough, and W. J. Lucas A Systemic Small RNA Signaling System in Plants PLANT CELL, August 1, 2004; 16(8): 1979 - 2000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Sena, J. W. Jung, and P. N. Benfey A broad competence to respond to SHORT ROOT revealed by tissue-specific ectopic expression Development, June 15, 2004; 131(12): 2817 - 2826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Engstrom, A. Izhaki, and J. L. Bowman Promoter Bashing, microRNAs, and Knox Genes. New Insights, Regulators, and Targets-of-Regulation in the Establishment of Lateral Organ Polarity in Arabidopsis Plant Physiology, June 1, 2004; 135(2): 685 - 694. [Full Text] [PDF]
|
|
|
|
|
|
G.-W. Tian, A. Mohanty, S. N. Chary, S. Li, B. Paap, G. Drakakaki, C. D. Kopec, J. Li, D. Ehrhardt, D. Jackson, et al. High-Throughput Fluorescent Tagging of Full-Length Arabidopsis Gene Products in Planta Plant Physiology, May 1, 2004; 135(1): 25 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Zambryski Cell-to-cell transport of proteins and fluorescent tracers via plasmodesmata during plant development J. Cell Biol., January 19, 2004; 164(2): 165 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-H. Cha, K. S. Cho, J. H. Lee, M. Kim, E. Kim, J. Park, S. B. Lee, and J. Chung Discrete Functions of TRAF1 and TRAF2 in Drosophila melanogaster Mediated by c-Jun N-Terminal Kinase and NF-{kappa}B-Dependent Signaling Pathways Mol. Cell. Biol., November 15, 2003; 23(22): 7982 - 7991. [Abstract] [Full Text] [PDF]
|
|
