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First published online 16 September 2003
doi: 10.1242/dev.00758
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1 Max Planck Institute of Molecular Cell Biology and Genetics,
Pfotenhauerstrasse 108, 01307 Dresden, Germany
2 University College London, Department of Anatomy and Developmental Biology,
Gower Street, London WC1E 6BT, UK
3 Programa de Morfologia, Instituto de Ciencias Biomedicas, Facultad de
Medicina, Universidad de Chile, PO Box 70079, Santiago de Chile, Chile
4 University of Iowa, Department of Biological Sciences, Iowa City, IA 52242,
USA
5 Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35 / III,
72076 Tübingen, Germany
6 University of Cambridge, Department of Anatomy, Downing Street, Cambridge CB2
3DY, UK
Author for correspondence (e-mail:
heisenberg{at}mpi-cbg.de)
Accepted 21 July 2003
During vertebrate gastrulation, highly coordinated cellular rearrangements lead to the formation of the three germ layers, ectoderm, mesoderm and endoderm. In zebrafish, silberblick (slb)/wnt11 regulates normal gastrulation movements by activating a signalling pathway similar to the Frizzled-signalling pathway, which establishes epithelial planar cell polarity (PCP) in Drosophila. However, the cellular mechanisms by which slb/wnt11 functions during zebrafish gastrulation are still unclear. Using high-resolution two-photon confocal imaging followed by computer-assisted reconstruction and motion analysis, we have analysed the movement and morphology of individual cells in three dimensions during the course of gastrulation. We show that in slb-mutant embryos, hypoblast cells within the forming germ ring have slower, less directed migratory movements at the onset of gastrulation. These aberrant cell movements are accompanied by defects in the orientation of cellular processes along the individual movement directions of these cells. We conclude that slb/wnt11-mediated orientation of cellular processes plays a role in facilitating and stabilising movements of hypoblast cells in the germ ring, thereby pointing at a novel function of the slb/wnt11 signalling pathway for the regulation of migratory cell movements at early stages of gastrulation.
Key words: Wnt signalling, Cell migration, Gastrulation, Zebrafish
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