|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online November 3, 2003
doi: 10.1242/10.1242/dev.00834
1 Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77
Stockholm, Sweden
2 Department of Neuroscience, Medical Nobel Institute, Karolinska Institute,
SE-171 77 Stockholm, Sweden
* Author for correspondence (e-mail: Urban.Lendahl{at}cmb.ki.se)
Accepted 28 August 2003
The bone morphogenetic protein (BMP) and Notch signaling pathways are
crucial for cellular differentiation. In many cases, the two pathways act
similarly; for example, to inhibit myogenic differentiation. It is not known
whether this inhibition is caused by distinct mechanisms or by an interplay
between Notch and BMP signaling. Here we demonstrate that functional Notch
signaling is required for BMP4-mediated block of differentiation of muscle
stem cells, i.e. satellite cells and the myogenic cell line C2C12. Addition of
BMP4 during induction of differentiation dramatically reduced the number of
differentiated satellite and C2C12 cells. Differentiation was substantially
restored in BMP4-treated cultures by blocking Notch signaling using either the
-secretase inhibitor L-685,458 or by introduction of a
dominant-negative version of the Notch signal mediator CSL. BMP4 addition to
C2C12 cells increased transcription of two immediate Notch responsive genes,
Hes1 and Hey1, an effect that was abrogated by L-685,458. A
3 kb Hey1-promoter reporter construct was synergistically activated
by the Notch 1 intracellular domain (Notch 1 ICD) and BMP4. The BMP4 mediator
SMAD1 mimicked BMP activation of the Hey1 promoter. A synthetic
Notch-responsive promoter containing no SMAD1 binding sites responded to
SMAD1, indicating that DNA-binding activity of SMAD1 is not required for
activation. Accordingly, Notch 1 ICD and SMAD1 interacted in binding
experiments in vitro. Thus, the data presented here provide evidence for a
direct interaction between the Notch and BMP signaling pathways, and indicate
that Notch has a crucial role in the execution of certain aspects of
BMP-mediated differentiation control.
Key words: SMAD, Delta, Serrate, TGFß, skeletal muscle, -secretase inhibitor
This article has been cited by other articles:
|
|
 |
|
  I. Imayoshi, T. Shimogori, T. Ohtsuka, and R. Kageyama Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline Development, August 1, 2008; 135(15): 2531 - 2541. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Niessen, Y. Fu, L. Chang, P. A. Hoodless, D. McFadden, and A. Karsan Slug is a direct Notch target required for initiation of cardiac cushion cellularization J. Cell Biol., July 28, 2008; 182(2): 315 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jin, E. M. Hansson, S. Tikka, F. Lanner, C. Sahlgren, F. Farnebo, M. Baumann, H. Kalimo, and U. Lendahl Notch Signaling Regulates Platelet-Derived Growth Factor Receptor-{beta} Expression in Vascular Smooth Muscle Cells Circ. Res., June 20, 2008; 102(12): 1483 - 1491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sakamoto, Y. Tamamura, K.-i. Katsube, and A. Yamaguchi Zfp64 participates in Notch signaling and regulates differentiation in mesenchymal cells J. Cell Sci., May 15, 2008; 121(10): 1613 - 1623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Kim, H. Neiswender, E. J. Baik, W. C. Xiong, and L. Mei {beta}-Catenin Interacts with MyoD and Regulates Its Transcription Activity Mol. Cell. Biol., May 1, 2008; 28(9): 2941 - 2951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zheng, S. Linke, J. M. Dias, X. Zheng, K. Gradin, T. P. Wallis, B. R. Hamilton, M. Gustafsson, J. L. Ruas, S. Wilkins, et al. Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways PNAS, March 4, 2008; 105(9): 3368 - 3373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. B. Yu, D. Y. Deng, H. Beppu, C. C. Hong, C. Lai, S. A. Hoyng, N. Kawai, and K. D. Bloch Bone Morphogenetic Protein (BMP) Type II Receptor Is Required for BMP-mediated Growth Arrest and Differentiation in Pulmonary Artery Smooth Muscle Cells J. Biol. Chem., February 15, 2008; 283(7): 3877 - 3888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hayashi, S. Maeda, H. Aburatani, K. Kitamura, H. Miyoshi, K. Miyazono, and T. Imamura Pitx2 Prevents Osteoblastic Transdifferentiation of Myoblasts by Bone Morphogenetic Proteins J. Biol. Chem., January 4, 2008; 283(1): 565 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-i. Fukada, A. Uezumi, M. Ikemoto, S. Masuda, M. Segawa, N. Tanimura, H. Yamamoto, Y. Miyagoe-Suzuki, and S. Takeda Molecular Signature of Quiescent Satellite Cells in Adult Skeletal Muscle Stem Cells, October 1, 2007; 25(10): 2448 - 2459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Geffers, K. Serth, G. Chapman, R. Jaekel, K. Schuster-Gossler, R. Cordes, D. B. Sparrow, E. Kremmer, S. L. Dunwoodie, T. Klein, et al. Divergent functions and distinct localization of the Notch ligands DLL1 and DLL3 in vivo J. Cell Biol., July 24, 2007; 178(3): 465 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pescatori, A. Broccolini, C. Minetti, E. Bertini, C. Bruno, A. D'amico, C. Bernardini, M. Mirabella, G. Silvestri, V. Giglio, et al. Gene expression profiling in the early phases of DMD: a constant molecular signature characterizes DMD muscle from early postnatal life throughout disease progression FASEB J, April 1, 2007; 21(4): 1210 - 1226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. L. Aranguren, A. Luttun, C. Clavel, C. Moreno, G. Abizanda, M. A. Barajas, B. Pelacho, M. Uriz, M. Arana, A. Echavarri, et al. In vitro and in vivo arterial differentiation of human multipotent adult progenitor cells Blood, March 15, 2007; 109(6): 2634 - 2642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Dyczynska, D. Sun, H. Yi, A. Sehara-Fujisawa, C. P. Blobel, and A. Zolkiewska Proteolytic Processing of Delta-like 1 by ADAM Proteases J. Biol. Chem., January 5, 2007; 282(1): 436 - 444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Carey, M. M. Farnfield, S. D. Tarquinio, and D. Cameron-Smith Impaired Expression of Notch Signaling Genes in Aged Human Skeletal Muscle J. Gerontol. A Biol. Sci. Med. Sci., January 1, 2007; 62(1): 9 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chapman, L. Liu, C. Sahlgren, C. Dahlqvist, and U. Lendahl High levels of Notch signaling down-regulate Numb and Numblike J. Cell Biol., November 20, 2006; 175(4): 535 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Y. Frank, A. T. Kho, T. Schatton, G. F. Murphy, M. J. Molloy, Q. Zhan, M. F. Ramoni, M. H. Frank, I. S. Kohane, and E. Gussoni Regulation of myogenic progenitor proliferation in human fetal skeletal muscle by BMP4 and its antagonist Gremlin J. Cell Biol., October 9, 2006; 175(1): 99 - 110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Sinha-Hikim, M. Cornford, H. Gaytan, M. L. Lee, and S. Bhasin Effects of Testosterone Supplementation on Skeletal Muscle Fiber Hypertrophy and Satellite Cells in Community-Dwelling Older Men J. Clin. Endocrinol. Metab., August 1, 2006; 91(8): 3024 - 3033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ta, Y. Choi, F. Atouf, C. H. Park, and N. Lumelsky The Defined Combination of Growth Factors Controls Generation of Long-Term-Replicating Islet Progenitor-Like Cells from Cultures of Adult Mouse Pancreas Stem Cells, July 1, 2006; 24(7): 1738 - 1749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Moriyama, M. Osawa, S.-S. Mak, T. Ohtsuka, N. Yamamoto, H. Han, V. Delmas, R. Kageyama, F. Beermann, L. Larue, et al. Notch signaling via Hes1 transcription factor maintains survival of melanoblasts and melanocyte stem cells J. Cell Biol., May 8, 2006; 173(3): 333 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Watanabe, H. Kokubo, S. Miyagawa-Tomita, M. Endo, K. Igarashi, K. i. Aisaki, J. Kanno, and Y. Saga Activation of Notch1 signaling in cardiogenic mesoderm induces abnormal heart morphogenesis in mouse Development, May 1, 2006; 133(9): 1625 - 1634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Doi, T. Iso, M. Yamazaki, H. Akiyama, H. Kanai, H. Sato, K. Kawai-Kowase, T. Tanaka, T. Maeno, E.-i. Okamoto, et al. HERP1 Inhibits Myocardin-Induced Vascular Smooth Muscle Cell Differentiation by Interfering With SRF Binding to CArG Box Arterioscler. Thromb. Vasc. Biol., November 1, 2005; 25(11): 2328 - 2334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kislinger, A. O. Gramolini, Y. Pan, K. Rahman, D. H. MacLennan, and A. Emili Proteome Dynamics during C2C12 Myoblast Differentiation Mol. Cell. Proteomics, July 1, 2005; 4(7): 887 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Matsuda, Y. Wakamatsu, J. Kohyama, H. Okano, K. Fukuda, and S. Yasugi Notch signaling functions as a binary switch for the determination of glandular and luminal fates of endodermal epithelium during chicken stomach development Development, June 15, 2005; 132(12): 2783 - 2793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nobta, T. Tsukazaki, Y. Shibata, C. Xin, T. Moriishi, S. Sakano, H. Shindo, and A. Yamaguchi Critical Regulation of Bone Morphogenetic Protein-induced Osteoblastic Differentiation by Delta1/Jagged1-activated Notch1 Signaling J. Biol. Chem., April 22, 2005; 280(16): 15842 - 15848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fuke, N. Sasagawa, and S. Ishiura Identification and Characterization of the Hesr1/Hey1 as a Candidate trans-Acting Factor on Gene Expression through the 3' Non-Coding Polymorphic Region of the Human Dopamine Transporter (DAT1) Gene J. Biochem., February 1, 2005; 137(2): 205 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Zamurovic, D. Cappellen, D. Rohner, and M. Susa Coordinated Activation of Notch, Wnt, and Transforming Growth Factor-{beta} Signaling Pathways in Bone Morphogenic Protein 2-induced Osteogenesis: Notch TARGET GENE Hey1 INHIBITS MINERALIZATION AND Runx2 TRANSCRIPTIONAL ACTIVITY J. Biol. Chem., September 3, 2004; 279(36): 37704 - 37715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Miller and C. P. Emerson Jr. Does the Road to Muscle Rejuvenation Go Through Notch? Sci. Aging Knowl. Environ., December 3, 2003; 2003(48): pe34 - 34. [Abstract] [Full Text]
|
|
