QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 5 November 2003
doi: 10.1242/dev.00868

This Article Figures Only Full Text Full Text (PDF) Supplemental Data All Versions of this Article: dev.00868v1 130/25/6221    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liu, R. Articles by Qiu, M. Search for Related Content PubMed PubMed Citation Articles by Liu, R. Articles by Qiu, M.

Region-specific and stage-dependent regulation of Olig gene expression and oligodendrogenesis by Nkx6.1 homeodomain transcription factor

Rugao Liu^1,*, Jun Cai^1,*, Xuemei Hu^1,*, Min Tan¹, Yingchuan Qi¹, Michael German², John Rubenstein³, Maike Sander⁴ and Mengsheng Qiu^1,

¹ Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, KY 40292, USA
² Department of Medicine, University of California, San Francisco, CA 94143, USA
³ Department of Psychiatry, University of California, San Francisco, CA 94143, USA
⁴ Department of Developmental and Cell Biology, University of California at Irvine, 4228 McGaugh Hall, Irvine CA 92697-2300, USA

Author for correspondence (e-mail: m0qiu001{at}louisville.edu)

Accepted 10 September 2003

During early neural development, the Nkx6.1 homeodomain neural progenitor gene is specifically expressed in the ventral neural tube, and its activity is required for motoneuron generation in the spinal cord. We report that Nkx6.1 also controls oligodendrocyte development in the developing spinal cord, possibly by regulating Olig gene expression in the ventral neuroepithelium. In Nkx6.1 mutant spinal cords, expression of Olig2 in the motoneuron progenitor domain is diminished, and the generation and differentiation of oligodendrocytes are significantly delayed and reduced. The regulation of Olig gene expression by Nkx6.1 is stage dependent, as ectopic expression of Nkx6.1 in embryonic chicken spinal cord results in an induction of Olig2 expression at early stages, but an inhibition at later stages. Moreover, the regulation of Olig gene expression and oligodendrogenesis by Nkx6.1 also appears to be region specific. In the hindbrain, unlike in the spinal cord, Olig1 and Olig2 can be expressed both inside and outside the Nkx6.1-expressing domains and oligodendrogenesis in this region is not dependent on Nkx6.1 activity.

Key words: Oligodendrocyte development, Nkx6.1 mutation, In ovo electroporation, Spinal cord, Olig2, Nkx2.2

This article has been cited by other articles:

				S. Yoshimura, J. I. Murray, Y. Lu, R. H. Waterston, and S. Shaham mls-2 and vab-3 control glia development, hlh-17/Olig expression and glia-dependent neurite extension in C. elegans Development, July 1, 2008; 135(13): 2263 - 2275. [Abstract] [Full Text] [PDF]

				M. Sugimori, M. Nagao, C. M. Parras, H. Nakatani, M. Lebel, F. Guillemot, and M. Nakafuku Ascl1 is required for oligodendrocyte development in the spinal cord Development, April 1, 2008; 135(7): 1271 - 1281. [Abstract] [Full Text] [PDF]

				M. Sugimori, M. Nagao, N. Bertrand, C. M. Parras, F. Guillemot, and M. Nakafuku Combinatorial actions of patterning and HLH transcription factors in the spatiotemporal control of neurogenesis and gliogenesis in the developing spinal cord Development, April 15, 2007; 134(8): 1617 - 1629. [Abstract] [Full Text] [PDF]

				L. Chi, Y. Ke, C. Luo, B. Li, D. Gozal, B. Kalyanaraman, and R. Liu Motor Neuron Degeneration Promotes Neural Progenitor Cell Proliferation, Migration, and Neurogenesis in the Spinal Cords of Amyotrophic Lateral Sclerosis Mice Stem Cells, January 1, 2006; 24(1): 34 - 43. [Abstract] [Full Text] [PDF]