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First published online 5 November 2003
doi: 10.1242/dev.00874

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The glypican Dally-like is required for Hedgehog signalling in the embryonic epidermis of Drosophila

Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK

^* Author for correspondence (e-mail: bs251{at}mole.bio.cam.ac.uk)

Accepted 2 September 2003

The Drosophila genes dally and dally-like encode glypicans, which are heparan sulphate proteoglycans anchored to the cell membrane by a glycosylphosphatidylinositol link. Genetic studies have implicated Dally and Dally-like in Wingless signalling in embryos and imaginal discs. Here, we test the signalling properties of these molecules in the embryonic epidermis. We demonstrate that RNA interference silencing of dally-like, but not dally, gives a segment polarity phenotype identical to that of null mutations in wingless or hedgehog. Using heterologous expression in embryos, we uncoupled the Hedgehog and Wingless signalling pathways and found that Dally-like and Dally, separately or together, are not necessary for Wingless signalling. Dally-like, however, is strictly necessary for Hedgehog signal transduction. Epistatic experiments show that Dally-like is required for the reception of the Hedgehog signal, upstream or at the level of the Patched receptor.

Key words: Dally, Wingless, heparan sulphate proteoglycan, signal transduction, RNA interference

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