|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online November 17, 2003
doi: 10.1242/10.1242/dev.00881
1 Center for Molecular Neurobiology Hamburg, University of Hamburg, Falkenried
94, D-20251 Hamburg, Germany
2 Instituto de Biología y Genética Molecular, Universidad de
Valladolid y Consejo Superior de Investigaciones Cientificas, Departamento de
Bioquímica, Biología Molecular y Fisiología, Facultad de
Medicina, E-47005 Valladolid, Spain
3 Institute for Animal Developmental and Molecular Biology,
Heinrich-Heine-University, D-40225 Düsseldorf, Germany
4 Institute of Molecular and Cellular Biosciences, University of Tokyo,
Bunkyo-Ku, Tokyo 113, Japan
5 School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG,
UK
Author for correspondence (e-mail:
schimman{at}epos.zmnh.uni-hamburg.de)
Accepted 23 September 2003
Members of the fibroblast growth factor (FGF) gene family control formation of the body plan and organogenesis in vertebrates. FGF3 is expressed in the developing hindbrain and has been shown to be involved in inner ear development of different vertebrate species, including zebrafish, Xenopus, chick and mouse. In the mouse, insertion of a neomycin resistance gene into the Fgf3 gene via homologous recombination results in severe developmental defects during differentiation of the otic vesicle. We have addressed the precise roles of FGF3 and other FGF family members during formation of the murine inner ear using both loss- and gain-of-function experiments. We generated a new mutant allele lacking the entire FGF3-coding region but surprisingly found no evidence for severe defects either during inner ear development or in the mature sensory organ, suggesting the functional involvement of other FGF family members during its formation. Ectopic expression of FGF10 in the developing hindbrain of transgenic mice leads to the formation of ectopic vesicles, expressing some otic marker genes and thus indicating a role for FGF10 during otic vesicle formation. Expression analysis of FGF10 during mouse embryogenesis reveals a highly dynamic pattern of expression in the developing hindbrain, partially overlapping with FGF3 expression and coinciding with formation of the inner ear. However, FGF10 mutant mice have been reported to display only mild defects during inner ear differentiation. We thus created double mutant mice for FGF3 and FGF10, which form severely reduced otic vesicles, suggesting redundant roles of these FGFs, acting in combination as neural signals for otic vesicle formation.
Key words: Fibroblast growth factor, Otic vesicle, Hindbrain, Mouse
This article has been cited by other articles:
|
|
 |
|
  S. Freter, Y. Muta, S.-S. Mak, S. Rinkwitz, and R. K. Ladher Progressive restriction of otic fate: the role of FGF and Wnt in resolving inner ear potential Development, October 15, 2008; 135(20): 3415 - 3424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hayashi, C. A. Ray, and O. Bermingham-McDonogh Fgf20 Is Required for Sensory Epithelial Specification in the Developing Cochlea J. Neurosci., June 4, 2008; 28(23): 5991 - 5999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. P. Hatch, C. A. Noyes, X. Wang, T. J. Wright, and S. L. Mansour Fgf3 is required for dorsal patterning and morphogenesis of the inner ear epithelium Development, October 15, 2007; 134(20): 3615 - 3625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hans and M. Westerfield Changes in retinoic acid signaling alter otic patterning Development, July 1, 2007; 134(13): 2449 - 2458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. S. Aggarwal, J. Liao, A. Bondarev, T. Schimmang, M. Lewandoski, J. Locker, A. Shanske, M. Campione, and B. E. Morrow Dissection of Tbx1 and Fgf interactions in mouse models of 22q11DS suggests functional redundancy Hum. Mol. Genet., November 1, 2006; 15(21): 3219 - 3228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Marguerie, F. Bajolle, S. Zaffran, N. A. Brown, C. Dickson, M. E. Buckingham, and R. G. Kelly Congenital heart defects in Fgfr2-IIIb and Fgf10 mutant mice Cardiovasc Res, July 1, 2006; 71(1): 50 - 60. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ohyama, O. A. Mohamed, M. M. Taketo, D. Dufort, and A. K. Groves Wnt signals mediate a fate decision between otic placode and epidermis Development, March 1, 2006; 133(5): 865 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nechiporuk, T. Linbo, and D. W. Raible Endoderm-derived Fgf3 is necessary and sufficient for inducing neurogenesis in the epibranchial placodes in zebrafish Development, August 15, 2005; 132(16): 3717 - 3730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Riccomagno, S. Takada, and D. J. Epstein Wnt-dependent regulation of inner ear morphogenesis is balanced by the opposing and supporting roles of Shh Genes & Dev., July 1, 2005; 19(13): 1612 - 1623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Lin, R. Cantos, M. Patente, and D. K. Wu Gbx2 is required for the morphogenesis of the mouse inner ear: a downstream candidate of hindbrain signaling Development, May 15, 2005; 132(10): 2309 - 2318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Ladher, T. J. Wright, A. M. Moon, S. L. Mansour, and G. C. Schoenwolf FGF8 initiates inner ear induction in chick and mouse Genes & Dev., March 1, 2005; 19(5): 603 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Mackereth, S.-J. Kwak, A. Fritz, and B. B. Riley Zebrafish pax8 is required for otic placode induction and plays a redundant role with Pax2 genes in the maintenance of the otic placode Development, January 15, 2005; 132(2): 371 - 382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hans, D. Liu, and M. Westerfield Pax8 and Pax2a function synergistically in otic specification, downstream of the Foxi1 and Dlx3b transcription factors Development, October 15, 2004; 131(20): 5091 - 5102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. F. Barald and M. W. Kelley From placode to polarization: new tunes in inner ear development Development, September 1, 2004; 131(17): 4119 - 4130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Chang, J. V. Brigande, D. M. Fekete, and D. K. Wu The development of semicircular canals in the inner ear: role of FGFs in sensory cristae Development, September 1, 2004; 131(17): 4201 - 4211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Saint-Germain, Y.-H. Lee, Y. Zhang, T. D. Sargent, and J.-P. Saint-Jeannet Specification of the otic placode depends on Sox9 function in Xenopus Development, April 15, 2004; 131(8): 1755 - 1763. [Abstract] [Full Text] [PDF]
|
|
