|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
doi: 10.1242/10.1242/dev.00366
1 Howard Hughes Medical Institute, 9 Cambridge Center, Cambridge, MA 02142,
USA
2 Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA
02142, USA
3 Department of Biology, Massachusetts Institute of Technology, Cambridge, MA
02139, USA
4 Institute for Biogenesis Research and Department of Anatomy and Reproductive
Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI
96822, USA
* Author for correspondence (e-mail: jaenisch{at}wi.mit.edu)
Accepted 8 January 2003
The majority of cloned animals derived by nuclear transfer from somatic cell nuclei develop to the blastocyst stage but die after implantation. Mouse embryos that lack an Oct4 gene, which plays an essential role in control of developmental pluripotency, develop to the blastocyst stage and also die after implantation, because they lack pluripotent embryonic cells. Based on this similarity, we posited that cloned embryos derived from differentiated cell nuclei fail to establish a population of truly pluripotent embryonic cells because of faulty reactivation of key embryonic genes such as Oct4. To explore this hypothesis, we used an in silico approach to identify a set of Oct4-related genes whose developmental expression pattern is similar to that of Oct4. When expression of Oct4 and 10 Oct4-related genes was analyzed in individual cumulus cell-derived cloned blastocysts, only 62% correctly expressed all tested genes. In contrast to this incomplete reactivation of Oct4-related genes in somatic clones, ES cell-derived cloned blastocysts and normal control embryos expressed these genes normally. Notably, the contrast between expression patterns of the Oct4-related genes correlated with efficiency of embryonic development of somatic and ES cell-derived cloned blastocysts to term. These observations suggest that failure to reactivate the full spectrum of these Oct4-related genes may contribute to embryonic lethality in somatic-cell clones.
Key words: Nuclear cloning, Gene expression, Developmental pluripotency, Oct4, Embryogenesis
Related articles in Development:
This article has been cited by other articles:
|
|
 |
|
  M. L. Condic and M. Rao Regulatory Issues for Personalized Pluripotent Cells Stem Cells, November 1, 2008; 26(11): 2753 - 2758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Do, D. W. Han, L. Gentile, I. Sobek-Klocke, M. Stehling, and H. R. Scholer Enhanced Reprogramming of Xist by Induced Upregulation of Tsix and Dnmt3a Stem Cells, November 1, 2008; 26(11): 2821 - 2831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kurimoto, Y. Yabuta, Y. Ohinata, M. Shigeta, K. Yamanaka, and M. Saitou Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice Genes & Dev., June 15, 2008; 22(12): 1617 - 1635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Monk, M. Hitchins, and S. Hawes Differential expression of the embryo/cancer gene ECSA(DPPA2), the cancer/testis gene BORIS and the pluripotency structural gene OCT4, in human preimplantation development Mol. Hum. Reprod., June 1, 2008; 14(6): 347 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. John, O. L. Caballero, S. J. Svobodova, A. Kong, R. Chua, J. Browning, S. Fortunato, S. Deb, M. Hsu, C. A. Gedye, et al. ECSA/DPPA2 is an Embryo-Cancer Antigen that Is Coexpressed with Cancer-Testis Antigens in Non-Small Cell Lung Cancer Clin. Cancer Res., June 1, 2008; 14(11): 3291 - 3298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ruau, R. Ensenat-Waser, T. C. Dinger, D. S. Vallabhapurapu, A. Rolletschek, C. Hacker, T. Hieronymus, A. M. Wobus, A. M. Muller, and M. Zenke Pluripotency Associated Genes Are Reactivated by Chromatin-Modifying Agents in Neurosphere Cells Stem Cells, April 1, 2008; 26(4): 920 - 926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Jincho, Y. Sotomaru, M. Kawahara, Y. Ono, H. Ogawa, Y. Obata, and T. Kono Identification of Genes Aberrantly Expressed in Mouse Embryonic Stem Cell-Cloned Blastocysts Biol Reprod, April 1, 2008; 78(4): 568 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hikichi, T. Kohda, S. Wakayama, F. Ishino, and T. Wakayama Nuclear Transfer Alters the DNA Methylation Status of Specific Genes in Fertilized and Parthenogenetically Activated Mouse Embryonic Stem Cells Stem Cells, March 1, 2008; 26(3): 783 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Li, M.-T. Pu, R. Hirasawa, B.-Z. Li, Y.-N. Huang, R. Zeng, N.-H. Jing, T. Chen, E. Li, H. Sasaki, et al. Synergistic Function of DNA Methyltransferases Dnmt3a and Dnmt3b in the Methylation of Oct4 and Nanog Mol. Cell. Biol., December 15, 2007; 27(24): 8748 - 8759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zhao, S. A Amiel, M. R Christie, P. Muiesan, P. Srinivasan, W. Littlejohn, M. Rela, M. Arno, N. Heaton, and G. C. Huang Evidence for the presence of stem cell-like progenitor cells in human adult pancreas J. Endocrinol., December 1, 2007; 195(3): 407 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Facucho-Oliveira, J. Alderson, E. C. Spikings, S. Egginton, and J. C. St. John Mitochondrial DNA replication during differentiation of murine embryonic stem cells J. Cell Sci., November 15, 2007; 120(22): 4025 - 4034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Masaki, T. Nishida, S. Kitajima, K. Asahina, and H. Teraoka Developmental Pluripotency-associated 4 (DPPA4) Localized in Active Chromatin Inhibits Mouse Embryonic Stem Cell Differentiation into a Primitive Ectoderm Lineage J. Biol. Chem., November 9, 2007; 282(45): 33034 - 33042. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Bowles, J.-H. Lee, R. Alberio, R. E. I. Lloyd, D. Stekel, K. H. S. Campbell, and J. C. St. John Contrasting Effects of in Vitro Fertilization and Nuclear Transfer on the Expression of mtDNA Replication Factors Genetics, July 1, 2007; 176(3): 1511 - 1526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wuensch, F. A. Habermann, S. Kurosaka, R. Klose, V. Zakhartchenko, H.-D. Reichenbach, F. Sinowatz, K. J. McLaughlin, and E. Wolf Quantitative Monitoring of Pluripotency Gene Activation after Somatic Cloning in Cattle Biol Reprod, June 1, 2007; 76(6): 983 - 991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kaji, J. Nichols, and B. Hendrich Mbd3, a component of the NuRD co-repressor complex, is required for development of pluripotent cells Development, March 15, 2007; 134(6): 1123 - 1132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Misica-Turner, F. C. Oback, M. Eichenlaub, D. N. Wells, and B. Oback Aggregating Embryonic but Not Somatic Nuclear Transfer Embryos Increases Cloning Efficiency in Cattle Biol Reprod, February 1, 2007; 76(2): 268 - 278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Smith, D. Berg, S. Beaumont, N. T Standley, D. N Wells, and P. L Pfeffer Simultaneous gene quantitation of multiple genes in individual bovine nuclear transfer blastocysts Reproduction, January 1, 2007; 133(1): 231 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Maldonado-Saldivia, J. van den Bergen, M. Krouskos, M. Gilchrist, C. Lee, R. Li, A. H. Sinclair, M. A. Surani, and P. S. Western Dppa2 and Dppa4 Are Closely Linked SAP Motif Genes Restricted to Pluripotent Cells and the Germ Line Stem Cells, January 1, 2007; 25(1): 19 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Alberio, K. H Campbell, and A. D Johnson Reprogramming somatic cells into stem cells. Reproduction, November 1, 2006; 132(5): 709 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wu, Q. C.-K. Cheung, L. Wen, and J. Li A Growth-Maturation System That Enhances the Meiotic and Developmental Competence of Porcine Oocytes Isolated from Small Follicles Biol Reprod, October 1, 2006; 75(4): 547 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Blelloch, Z. Wang, A. Meissner, S. Pollard, A. Smith, and R. Jaenisch Reprogramming Efficiency Following Somatic Cell Nuclear Transfer Is Influenced by the Differentiation and Methylation State of the Donor Nucleus Stem Cells, September 1, 2006; 24(9): 2007 - 2013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ono and T. Kono Irreversible Barrier to the Reprogramming of Donor Cells in Cloning with Mouse Embryos and Embryonic Stem Cells Biol Reprod, August 1, 2006; 75(2): 210 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Somers, C. Smith, M. Donnison, D. N Wells, H. Henderson, L. McLeay, and P L Pfeffer Gene expression profiling of individual bovine nuclear transfer blastocysts. Reproduction, June 1, 2006; 131(6): 1073 - 1084. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yu, D. Fang, S. M. Kumar, L. Li, T. K. Nguyen, G. Acs, M. Herlyn, and X. Xu Isolation of a Novel Population of Multipotent Adult Stem Cells from Human Hair Follicles Am. J. Pathol., June 1, 2006; 168(6): 1879 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rybouchkin, Y. Kato, and Y. Tsunoda Role of Histone Acetylation in Reprogramming of Somatic Nuclei Following Nuclear Transfer Biol Reprod, June 1, 2006; 74(6): 1083 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Skrzyszowska, Z. Smorag, R. Slomski, L. Katska-Ksiazkiewicz, R. Kalak, E. Michalak, K. Wielgus, J. Lehmann, D. Lipinski, M. Szalata, et al. Generation of Transgenic Rabbits by the Novel Technique of Chimeric Somatic Cell Cloning Biol Reprod, June 1, 2006; 74(6): 1114 - 1120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pritsker, N. R. Ford, H. T. Jenq, and I. R. Lemischka Genomewide gain-of-function genetic screen identifies functionally active genes in mouse embryonic stem cells PNAS, May 2, 2006; 103(18): 6946 - 6951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N Cobbe Why the apparent haste to clone humans? J. Med. Ethics, May 1, 2006; 32(5): 298 - 302. [Full Text] [PDF]
|
|
|
|
|
|
N. Van Thuan, S. Wakayama, S. Kishigami, H. Ohta, T. Hikichi, E. Mizutani, H.-T. Bui, and T. Wakayama Injection of Somatic Cell Cytoplasm into Oocytes Before Intracytoplasmic Sperm Injection Impairs Full-Term Development and Increases Placental Weight in Mice Biol Reprod, May 1, 2006; 74(5): 865 - 873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jouneau, Q. Zhou, A. Camus, V. Brochard, L. Maulny, J. Collignon, and J.-P. Renard Developmental abnormalities of NT mouse embryos appear early after implantation. Development, April 1, 2006; 133(8): 1597 - 1607. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Master Embryonic stem-cell gametes: the new frontier in human reproduction Hum. Reprod., April 1, 2006; 21(4): 857 - 863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Brambrink, K. Hochedlinger, G. Bell, and R. Jaenisch ES cells derived from cloned and fertilized blastocysts are transcriptionally and functionally indistinguishable PNAS, January 24, 2006; 103(4): 933 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Elliman, I. Wu, and D. M. Kemp Adult Tissue-specific Expression of a Dppa3-derived Retrogene Represents a Postnatal Transcript of Pluripotent Cell Origin J. Biol. Chem., January 6, 2006; 281(1): 16 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yu, M. A. Vodyanik, P. He, I. I. Slukvin, and J. A. Thomson Human Embryonic Stem Cells Reprogram Myeloid Precursors Following Cell-Cell Fusion Stem Cells, January 1, 2006; 24(1): 168 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Juric, S. Sale, R. A. Hromas, R. Yu, Y. Wang, G. E. Duran, R. Tibshirani, L. H. Einhorn, and B. I. Sikic Gene expression profiling differentiates germ cell tumors from other cancers and defines subtype-specific signatures PNAS, December 6, 2005; 102(49): 17763 - 17768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kohda, K. Inoue, N. Ogonuki, H. Miki, M. Naruse, T. Kaneko-Ishino, A. Ogura, and F. Ishino Variation in Gene Expression and Aberrantly Regulated Chromosome Regions in Cloned Mice Biol Reprod, December 1, 2005; 73(6): 1302 - 1311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Western, J. Maldonado-Saldivia, J. van den Bergen, P. Hajkova, M. Saitou, S. Barton, and M. A. Surani Analysis of Esg1 Expression in Pluripotent Cells and the Germline Reveals Similarities with Oct4 and Sox2 and Differences Between Human Pluripotent Cell Lines Stem Cells, October 1, 2005; 23(10): 1436 - 1442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Boiani, L. Gentile, V. V. Gambles, F. Cavaleri, C. A. Redi, and H. R. Scholer Variable Reprogramming of the Pluripotent Stem Cell Marker Oct4 in Mouse Clones: Distinct Developmental Potentials in Different Culture Environments Stem Cells, September 1, 2005; 23(8): 1089 - 1104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yamazaki, E. W. Low, Y. Marikawa, K. Iwahashi, M. S. Bartolomei, J. R. McCarrey, and R. Yanagimachi Adult mice cloned from migrating primordial germ cells PNAS, August 9, 2005; 102(32): 11361 - 11366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Eckardt, N A. Leu, S. Kurosaka, and K J. McLaughlin Differential reprogramming of somatic cell nuclei after transfer into mouse cleavage stage blastomeres Reproduction, May 1, 2005; 129(5): 547 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. D. Morgan, F. Santos, K. Green, W. Dean, and W. Reik Epigenetic reprogramming in mammals Hum. Mol. Genet., April 15, 2005; 14(suppl_1): R47 - R58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-K. Kim, M. R. Suh, H. S. Yoon, J. B. Lee, S. K. Oh, S. Y. Moon, S.-H. Moon, J. Y. Lee, J. H. Hwang, W. J. Cho, et al. Identification of Developmental Pluripotency Associated 5 Expression in Human Pluripotent Stem Cells Stem Cells, April 1, 2005; 23(4): 458 - 462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wakayama, H. Ohta, S. Kishigami, N. Van Thuan, T. Hikichi, E. Mizutani, M. Miyake, and T. Wakayama Establishment of Male and Female Nuclear Transfer Embryonic Stem Cell Lines from Different Mouse Strains and Tissues Biol Reprod, April 1, 2005; 72(4): 932 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Ng and J. B. Gurdon Epigenetic memory of active gene transcription is inherited through somatic cell nuclear transfer PNAS, February 8, 2005; 102(6): 1957 - 1962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wakayama, S. Kishigami, N. Van Thuan, H. Ohta, T. Hikichi, E. Mizutani, R. Yanagimachi, and T. Wakayama From The Cover: Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology PNAS, January 4, 2005; 102(1): 29 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Prather, P. Sutovsky, and J. A. Green Nuclear Remodeling and Reprogramming in Transgenic Pig Production Experimental Biology and Medicine, December 1, 2004; 229(11): 1120 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Blelloch, K. Hochedlinger, Y. Yamada, C. Brennan, M. Kim, B. Mintz, L. Chin, and R. Jaenisch Inaugural Article: Nuclear cloning of embryonal carcinoma cells PNAS, September 28, 2004; 101(39): 13985 - 13990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kimura, M. Tada, N. Nakatsuji, and T. Tada Histone Code Modifications on Pluripotential Nuclei of Reprogrammed Somatic Cells Mol. Cell. Biol., July 1, 2004; 24(13): 5710 - 5720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C St John, R. E I Lloyd, E. J Bowles, E. C Thomas, and S. El Shourbagy The consequences of nuclear transfer for mammalian foetal development and offspring survival. A mitochondrial DNA perspective Reproduction, June 1, 2004; 127(6): 631 - 641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-C. Ng, N. Chen, W.-Y. Yip, S.-L. Liow, G.-Q. Tong, B. Martelli, L. G. Tan, and P. Martelli The first cell cycle after transfer of somatic cell nuclei in a non-human primate Development, May 15, 2004; 131(10): 2475 - 2484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. De Felici, M.L. Scaldaferri, M. Lobascio, S. Iona, V. Nazzicone, F.G. Klinger, and D. Farini Experimental approaches to the study of primordial germ cell lineage and proliferation Hum. Reprod. Update, May 1, 2004; 10(3): 197 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Koh and A. Atala Tissue Engineering, Stem Cells, and Cloning: Opportunities for Regenerative Medicine J. Am. Soc. Nephrol., May 1, 2004; 15(5): 1113 - 1125. [Full Text] [PDF]
|
|
|
|
 |
|
 R. JAENISCH, K. HOCHEDLINGER, R. BLELLOCH, Y. YAMADA, K. BALDWIN, and K. EGGAN Nuclear Cloning, Epigenetic Reprogramming, and Cellular Differentiation Cold Spring Harb Symp Quant Biol, January 1, 2004; 69(0): 19 - 28. [Abstract] [PDF]
|
|
|
|
 |
|
 K. G. Sylvester and M. T. Longaker Stem Cells: Review and Update Arch Surg, January 1, 2004; 139(1): 93 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Mitalipov, H.-C. Kuo, J. D. Hennebold, and D. P. Wolf Oct-4 Expression in Pluripotent Cells of the Rhesus Monkey Biol Reprod, December 1, 2003; 69(6): 1785 - 1792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R.W. Mann, Y. G. Chung, L. D. Nolen, R. I. Verona, K. E. Latham, and M. S. Bartolomei Disruption of Imprinted Gene Methylation and Expression in Cloned Preimplantation Stage Mouse Embryos Biol Reprod, September 1, 2003; 69(3): 902 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hochedlinger and R. Jaenisch Nuclear Transplantation, Embryonic Stem Cells, and the Potential for Cell Therapy N. Engl. J. Med., July 17, 2003; 349(3): 275 - 286. [Full Text] [PDF]
|
|
|
|
|
|
J. B. Gurdon and J. A. Byrne The first half-century of nuclear transplantation PNAS, July 8, 2003; 100(14): 8048 - 8052. [Abstract] [Full Text] [PDF]
|
|
