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doi: 10.1242/10.1242/dev.00418

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Development 130, 1853-1865 (2003)
Copyright © 2003 The Company of Biologists Limited

A homeobox gene, pnx, is involved in the formation of posterior neurons in zebrafish

Young-Ki Bae1,2, Takashi Shimizu1,2, Taijiro Yabe2, Cheol-Hee Kim4, Tsutomu Hirata2, Hideaki Nojima2, Osamu Muraoka1, Toshio Hirano2,3 and Masahiko Hibi1,2,*

1 Laboratory for Vertebrate Axis Formation, Center for Developmental Biology, RIKEN, Kobe, Hyogo 650-0047, Japan
2 Department of Molecular Oncology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan
3 Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan
4 Department of Biology, Chungnam National University, Daejeon 305-764, Korea

* Author for correspondence (e-mail: hibi{at}cdb.riken.go.jp)

Accepted 29 January 2003

A homeobox gene, pnx, is expressed in prospective posterior neurogenic regions and later in primary neurons. pnx expression was regulated by a signal from the non-axial mesendoderm and by Notch signaling. Pnx contains an Eh1 repressor domain, which interacted with Groucho and acted as a transcriptional repressor. Misexpression of pnx increased neural precursor cells and postmitotic neurons, which express neurogenin1 and elavl3/HuC, respectively. Expression of an antimorphic Pnx (VP16Pnx) or inhibition of Pnx by antisense morpholino oligonucleotide led to the reduction in the number of a subset of primary neurons. Misexpression of pnx promoted neurogenesis independent of Notch signaling. Epistatic analyses showed that Pnx also functions downstream of the Notch signal. These data indicate that pnx is a novel repressor-type homeobox gene that regulates posterior neurogenesis.

Key words: Homeobox, Proneural gene, Neurogenic gene, Zebrafish




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© The Company of Biologists Ltd 2003