|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
doi: 10.1242/10.1242/dev.00389
1 The Salk Institute for Biological Studies, PO Box 85800, San Diego, CA 92186,
USA
2 Department of Biology, University of California, Riverside, CA 92521,
USA
* Present address: McGill University Centre for Research in Neuroscience, 1650
Cedar Avenue, Montreal, QC H3G 1A4, Canada
Present address: CyThera, San Diego, CA 92121, USA
Author for correspondence (e-mail:
jthomas{at}salk.edu)
Accepted 29 January 2003
LIM-homeodomain transcription factors control a variety of developmental processes, and are assembled into functional complexes with the LIM-binding co-factor Ldb1 (in mouse) or Chip (in Drosophila). We describe the identification and characterization of members of the Ssdp family of proteins, which we show to interact with Ldb1 and Chip. The N terminus of Ssdp is highly conserved among species and binds a highly conserved domain within Ldb1/Chip that is distinct from the domains required for LIM binding and self-dimerization. In Drosophila, Ssdp is expressed in the developing nervous system and imaginal tissues, and it is capable of modifying the in vivo activity of complexes comprised of Chip and the LIM-homeodomain protein Apterous. Null mutations of the ssdp gene are cell-lethal in clones of cells within the developing wing disc. However, clones mutant for a hypomorphic allele give rise to ectopic margins, wing outgrowth and cell identity defects similar to those produced by mutant clones of Chip or apterous. Ssdp and Ldb/Chip each show structural similarity to two Arabidopsis proteins that cooperate with one another to regulate gene expression during flower development, suggesting that the molecular interactions between Ssdp and Ldb/Chip proteins are evolutionarily ancient and supply a fundamental function in the regulated control of transcription.
Key words: LIM domain, Homeodomain, Drosophila, Wing development, Apterous, Chip
This article has been cited by other articles:
|
|
 |
|
  S. Zenvirt, Y. Nevo-Caspi, S. Rencus-Lazar, and D. Segal Drosophila LIM-Only Is a Positive Regulator of Transcription During Thoracic Bristle Development Genetics, August 1, 2008; 179(4): 1989 - 1999. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Azhakanandam, S. Nole-Wilson, F. Bao, and R. G. Franks SEUSS and AINTEGUMENTA Mediate Patterning and Ovule Initiation during Gynoecium Medial Domain Development Plant Physiology, March 1, 2008; 146(3): 1165 - 1181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gungor, N. Taniguchi-Ishigaki, H. Ma, A. Drung, B. Tursun, H. P. Ostendorff, M. Bossenz, C. G. Becker, T. Becker, and I. Bach Proteasomal selection of multiprotein complexes recruited by LIM homeodomain transcription factors PNAS, September 18, 2007; 104(38): 15000 - 15005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. de Navascues and J. Modolell tailup, a LIM-HD gene, and Iro-C cooperate in Drosophila dorsal mesothorax specification Development, May 1, 2007; 134(9): 1779 - 1788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Xu, X. Meng, Y. Cai, H. Liang, L. Nagarajan, and S. J. Brandt Single-stranded DNA-binding proteins regulate the abundance of LIM domain and LIM domain-binding proteins Genes & Dev., April 15, 2007; 21(8): 942 - 955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Neduva and R. B. Russell Proline-Rich Regions in Transcriptional Complexes: Heading in Many Directions Sci. Signal., January 16, 2007; 2007(369): pe1 - pe1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Meier, S. Krpic, P. Rodriguez, J. Strouboulis, M. Monti, J. Krijgsveld, M. Gering, R. Patient, A. Hostert, and F. Grosveld Novel binding partners of Ldb1 are required for haematopoietic development Development, December 15, 2006; 133(24): 4913 - 4923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Enkhmandakh, A. V. Makeyev, and D. Bayarsaihan The role of the proline-rich domain of Ssdp1 in the modular architecture of the vertebrate head organizer PNAS, August 1, 2006; 103(31): 11631 - 11636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. H. Tran, Z. Xu, A. Kato, A. C. Mistry, Y. Goya, M. Taira, S. J. Brandt, and S. Hirose Spliced Isoforms of LIM-Domain-Binding Protein (CLIM/NLI/Ldb) Lacking the LIM-Interaction Domain J. Biochem., July 1, 2006; 140(1): 105 - 119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Cao, S. Lane, P. P. Raniga, Y. Lu, Z. Zhou, K. Ramon, J. Chen, and H. Liu The Flo8 Transcription Factor Is Essential for Hyphal Development and Virulence in Candida albicans Mol. Biol. Cell, January 1, 2006; 17(1): 295 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Schuh, A. J. Tipping, A. J. Clark, I. Hamlett, B. Guyot, F. J. Iborra, P. Rodriguez, J. Strouboulis, T. Enver, P. Vyas, et al. ETO-2 Associates with SCL in Erythroid Cells and Megakaryocytes and Provides Repressor Functions in Erythropoiesis Mol. Cell. Biol., December 1, 2005; 25(23): 10235 - 10250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nishioka, S. Nagano, R. Nakayama, H. Kiyonari, T. Ijiri, K. Taniguchi, W. Shawlot, Y. Hayashizaki, H. Westphal, R. R. Behringer, et al. Ssdp1 regulates head morphogenesis of mouse embryos by activating the Lim1-Ldb1 complex Development, June 1, 2005; 132(11): 2535 - 2546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Certel and S. Thor Specification of Drosophila motoneuron identity by the combinatorial action of POU and LIM-HD factors Development, November 1, 2004; 131(21): 5429 - 5439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. V. Sridhar, A. Surendrarao, D. Gonzalez, R. S. Conlan, and Z. Liu Transcriptional repression of target genes by LEUNIG and SEUSS, two interacting regulatory proteins for Arabidopsis flower development PNAS, August 3, 2004; 101(31): 11494 - 11499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. I. Pueyo and J. P. Couso Chip-mediated partnerships of the homeodomain proteins Bar and Aristaless with the LIM-HOM proteins Apterous and Lim1 regulate distal leg development Development, July 1, 2004; 131(13): 3107 - 3120. [Abstract] [Full Text] [PDF]
|
|
