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First published online 29 September 2004
doi: 10.1242/dev.01412

This Article Figures Only Full Text Full Text (PDF) Supplementary Material All Versions of this Article: dev.01412v1 131/21/5243    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pinheiro, E. M. Articles by Montell, D. J. Search for Related Content PubMed PubMed Citation Articles by Pinheiro, E. M. Articles by Montell, D. J.

Requirement for Par-6 and Bazooka in Drosophila border cell migration

Department of Biological Chemistry, Johns Hopkins School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205-2185, USA

^* Author for correspondence (e-mail: dmontell{at}jhmi.edu)

Accepted 20 August 2004

Polarized epithelial cells convert into migratory invasive cells during a number of developmental processes, as well as when tumors metastasize. Much has been learned recently concerning the molecules and mechanisms that are responsible for generating and maintaining epithelial cell polarity. However, less is known about what becomes of epithelial polarity proteins when various cell types become migratory and invasive. Here, we report the localization of several apical epithelial proteins, Par-6, Par-3/Bazooka and aPKC, during border cell migration in the Drosophila ovary. All of these proteins remained asymmetrically distributed throughout migration. Moreover, depletion of either Par-6 or Par-3/Bazooka by RNAi resulted in disorganization of the border cell cluster and impaired migration. The distributions of several transmembrane proteins required for migration were abnormal following Par-6 or Par-3/Bazooka downregulation, possibly accounting for the migration defects. Taken together, these results indicate that cells need not lose apical/basal polarity in order to invade neighboring tissues and in some cases even require such polarity for proper motility.

Key words: Par-6, Bazooka, Border cells, Cell migration, Drosophila

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