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First published online 2 December 2004
doi: 10.1242/dev.01564

Development 132, 49-60 (2005)
Published by The Company of Biologists 2005
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Sequential roles of Hedgehog and Wnt signaling in osteoblast development

Hongliang Hu1,*, Matthew J. Hilton1,*, Xiaolin Tu1, Kai Yu2, David M. Ornitz2 and Fanxin Long1,2,{dagger}

1 Department of Medicine, Washington University Medical School, St. Louis, MO 63110, USA
2 Department of Molecular Biology and Pharmacology, Washington University Medical School, St Louis, MO 63110, USA

{dagger} Author for correspondence (e-mail: flong{at}wustl.edu)

Accepted 3 November 2004

Signals that govern development of the osteoblast lineage are not well understood. Indian hedgehog (Ihh), a member of the hedgehog (Hh) family of proteins, is essential for osteogenesis in the endochondral skeleton during embryogenesis. The canonical pathway of Wnt signaling has been implicated by studies of Lrp5, a co-receptor for Wnt proteins, in postnatal bone mass homeostasis. In the present study we demonstrate that ß-catenin, a central player in the canonical Wnt pathway, is indispensable for osteoblast differentiation in the mouse embryo. Moreover, we present evidence that Wnt signaling functions downstream of Ihh in development of the osteoblast lineage. Finally Wnt7b is identified as a potential endogenous ligand regulating osteogenesis. These data support a model that integrates Hh and Wnt signaling in the regulation of osteoblast development.

Key words: Hh, Wnt, Osteoblast, Mouse




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