spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 18 May 2005
doi: 10.1242/dev.01856

Development 132, 2759-2770 (2005)
Published by The Company of Biologists 2005
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
dev.01856v1
132/12/2759    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kubo, F.
Right arrow Articles by Nakagawa, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kubo, F.
Right arrow Articles by Nakagawa, S.

Wnt2b inhibits differentiation of retinal progenitor cells in the absence of Notch activity by downregulating the expression of proneural genes

Fumi Kubo1,2,*, Masatoshi Takeichi1,2 and Shinichi Nakagawa1,*,{dagger}

1 RIKEN Center for Developmental Biology, 2-2-3 Minatojima Minamimachi, Chuo-ku, Kobe 650-0047, Japan
2 Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan

{dagger} Author for correspondence (e-mail: nakagawas{at}riken.jp)

Accepted 7 April 2005

During the development of the central nervous system, cell proliferation and differentiation are precisely regulated. In the vertebrate eye, progenitor cells located in the marginal-most region of the neural retina continue to proliferate for a much longer period compared to the ones in the central retina, thus showing stem-cell-like properties. Wnt2b is expressed in the anterior rim of the optic vesicles, and has been shown to control differentiation of the progenitor cells in the marginal retina. In this paper, we show that stable overexpression of Wnt2b in retinal explants inhibited cellular differentiation and induced continuous growth of the tissue. Notably, Wnt2b maintained the undifferentiated progenitor cells in the explants even under the conditions where Notch signaling was blocked. Wnt2b downregulated the expression of multiple proneural bHLH genes as well as Notch. In addition, expression of Cath5 under the control of an exogenous promoter suppressed the negative effect of Wnt2b on neuronal differentiation. Importantly, Wnt2b inhibited neuronal differentiation independently of cell cycle progression. We propose that Wnt2b maintains the naive state of marginal progenitor cells by attenuating the expression of both proneural and neurogenic genes, thus preventing those cells from launching out into the differentiation cascade regulated by proneural genes and Notch.

Key words: Wnt, retina, progenitor cells, stem cells, proneural gene, Notch, Delta, differentiation, cell cycle




This article has been cited by other articles:


Home page
 Stem CellsHome page
T. Denayer, M. Locker, C. Borday, T. Deroo, S. Janssens, A. Hecht, F. van Roy, M. Perron, and K. Vleminckx
Canonical Wnt Signaling Controls Proliferation of Retinal Stem/Progenitor Cells in Postembryonic Xenopus Eyes
Stem Cells, August 1, 2008; 26(8): 2063 - 2074.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
X. Zhao, A. V. Das, S. Bhattacharya, W. B. Thoreson, J. R. Sierra, K. B. Mallya, and I. Ahmad
Derivation of Neurons with Functional Properties from Adult Limbal Epithelium: Implications in Autologous Cell Therapy for Photoreceptor Degeneration
Stem Cells, April 1, 2008; 26(4): 939 - 949.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P. Hayward, T. Kalmar, and A. Martinez Arias
Wnt/Notch signalling and information processing during development
Development, February 1, 2008; 135(3): 411 - 424.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Y. Sokol and K. A. Wharton Jr
WNTers in La Jolla
Development, October 1, 2007; 134(19): 3393 - 3399.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. P. Jadhav, S.-H. Cho, and C. L. Cepko
Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property
PNAS, December 12, 2006; 103(50): 18998 - 19003.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
H. Liu, S. Thurig, O. Mohamed, D. Dufort, and V. A. Wallace
Mapping Canonical Wnt Signaling in the Developing and Adult Retina
Invest. Ophthalmol. Vis. Sci., November 1, 2006; 47(11): 5088 - 5097.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. Tanabe, Y. Takahashi, Y. Sato, K. Kawakami, M. Takeichi, and S. Nakagawa
Cadherin is required for dendritic morphogenesis and synaptic terminal organization of retinal horizontal cells
Development, October 15, 2006; 133(20): 4085 - 4096.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S.-H. Cho and C. L. Cepko
Wnt2b/{beta}-catenin-mediated canonical Wnt signaling determines the peripheral fates of the chick eye
Development, August 15, 2006; 133(16): 3167 - 3177.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Bossing and A. H. Brand
Determination of cell fate along the anteroposterior axis of the Drosophila ventral midline
Development, March 15, 2006; 133(6): 1001 - 1012.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. P. Jadhav, H. A. Mason, and C. L. Cepko
Notch 1 inhibits photoreceptor production in the developing mammalian retina
Development, March 1, 2006; 133(5): 913 - 923.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005