spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online June 8, 2005
doi: 10.1242/10.1242/dev.01886

Development 132, 3069-3077 (2005)
Published by The Company of Biologists 2005
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Development
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Medioni, C.
Right arrow Articles by Noselli, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Medioni, C.
Right arrow Articles by Noselli, S.

Dynamics of the basement membrane in invasive epithelial clusters in Drosophila

Caroline Medioni and Stéphane Noselli*

Institute of Signaling, Developmental Biology and Cancer, UMR 6543 CNRS, University of Nice Sophia-Antipolis, Parc Valrose, 06108 Nice, cedex 2, France

* Author for correspondence (e-mail: noselli{at}unice.fr)

Accepted 29 April 2005

The basement membrane (BM) represents a barrier to cell migration, which has to be degraded to promote invasion. However, the role and behaviour of the BM during the development of pre-invasive cells is only poorly understood. Drosophila border cells (BCs) provide an attractive genetic model in which to study the cellular mechanisms underlying the migration of mixed cohorts of epithelial cells. BCs are made of two different epithelial cell types appearing sequentially during oogenesis: the polar cells and the outer BCs. Here, we show that the pre-invasive polar cells undergo an unusual and asymmetrical apical capping with major basement membrane proteins, including the two Drosophila Collagen IV {alpha} chains, Laminin A and Perlecan. Capping of polar cells proceeds through a novel, basal-to-apical transcytosis mechanism that involves the small GTPase Drab5. Apical capping is transient and is followed by rapid shedding prior to the initiation of BC migration, suggesting that the apical cap blocks migration. Consistently, non-migratory polar cells remain capped. We further show that JAK/STAT signalling and recruitment of outer BCs are required for correct shedding and migration. The dynamics of the BM represents a marker of migratory BC, revealing a novel developmentally regulated behaviour of BM coupled to epithelial cell invasiveness.

Key words: Basement membrane, Border cells, Migration, JAK/STAT, Transcytosis, Collagen IV


Related articles in Development:

Membrane dynamics and cell invasion

Development 2005 132: e1303. [Full Text]  



This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
A. M. O'Reilly, H.-H. Lee, and M. A. Simon
Integrins control the positioning and proliferation of follicle stem cells in the Drosophila ovary
J. Cell Biol., August 25, 2008; 182(4): 801 - 815.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
O. Devergne, C. Ghiglione, and S. Noselli
The endocytic control of JAK/STAT signalling in Drosophila
J. Cell Sci., October 1, 2007; 120(19): 3457 - 3464.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Srivastava, J. C. Pastor-Pareja, T. Igaki, R. Pagliarini, and T. Xu
Basement membrane remodeling is essential for Drosophila disc eversion and tumor invasion
PNAS, February 20, 2007; 104(8): 2721 - 2726.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005