|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
First published online 5 January 2005
doi: 10.1242/dev.01593
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Medical Research Council, National Institute for Medical Research, The
Ridgeway, Mill Hill, London, NW7 1AA, UK
2 Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MI
64110, USA
3 Institut de Génétique et de Biologie Moléculaire et
Cellulaire (IGBMC), CNRS/INSERM/ULP/Collège de France, BP10142, 67404
ILLKIRCH Cedex, France
4 Department of Pathology and Laboratory Medicine, Kansas University Medical
Center, Kansas City, KS 66160, USA
5 Department of Anatomy and Cell Biology, Kansas University Medical Center,
Kansas City, KS 66160, USA
Author for correspondence (e-mail:
agould{at}nimr.mrc.ac.uk)
Accepted 23 November 2004
During anteroposterior (AP) patterning of the developing hindbrain, the expression borders of many transcription factors are aligned at interfaces between neural segments called rhombomeres (r). Mechanisms regulating segmental expression have been identified for Hox genes, but for other classes of AP patterning genes there is only limited information. We have analysed the murine retinoic acid receptor ß gene (Rarb) and show that it is induced prior to segmentation, by retinoic-acid (RA) signalling from the mesoderm. Induction establishes a diffuse expression border that regresses until, at later stages, it is stably maintained at the r6/r7 boundary by inputs from Hoxb4 and Hoxd4. Separate RA- and Hox-responsive enhancers mediate the two phases of Rarb expression: a regulatory mechanism remarkably similar to that of Hoxb4. By showing that Rarb is a direct transcriptional target of Hoxb4, this study identifies a new molecular link, completing a feedback circuit between Rarb, Hoxb4 and Hoxd4. We propose that the function of this circuit is to align the initially incongruent expression of multiple RA-induced genes at a single segment boundary.
Key words: Segmentation, Rhombomere, Hindbrain, Hox, Retinoic acid receptor
This article has been cited by other articles:
|
|
 |
|
  E. Kutejova, B. Engist, M. Self, G. Oliver, P. Kirilenko, and N. Bobola Six2 functions redundantly immediately downstream of Hoxa2 Development, April 15, 2008; 135(8): 1463 - 1470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Johansen, P. E. Hoyer, L. A. Larsen, P. A. Price, and K. Mollgard YKL-40 Protein Expression in the Early Developing Human Musculoskeletal System J. Histochem. Cytochem., December 1, 2007; 55(12): 1213 - 1228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. S. Daftary and H. S. Taylor Endocrine Regulation of HOX Genes Endocr. Rev., June 1, 2006; 27(4): 331 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Ashe and J. Briscoe The interpretation of morphogen gradients Development, February 1, 2006; 133(3): 385 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ferretti, F. Cambronero, S. Tumpel, E. Longobardi, L. M. Wiedemann, F. Blasi, and R. Krumlauf Hoxb1 Enhancer and Control of Rhombomere 4 Expression: Complex Interplay between PREP1-PBX1-HOXB1 Binding Sites Mol. Cell. Biol., October 1, 2005; 25(19): 8541 - 8552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Vermot, B. Schuhbaur, H. L. Mouellic, P. McCaffery, J.-M. Garnier, D. Hentsch, P. Brulet, K. Niederreither, P. Chambon, P. Dolle, et al. Retinaldehyde dehydrogenase 2 and Hoxc8 are required in the murine brachial spinal cord for the specification of Lim1+ motoneurons and the correct distribution of Islet1+ motoneurons Development, April 1, 2005; 132(7): 1611 - 1621. [Abstract] [Full Text] [PDF]
|
|
