spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online May 11, 2006
doi: 10.1242/10.1242/dev.02345

Development 133, 2105-2113 (2006)
Published by The Company of Biologists 2006
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wildner, H.
Right arrow Articles by Birchmeier, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wildner, H.
Right arrow Articles by Birchmeier, C.

dILA neurons in the dorsal spinal cord are the product of terminal and non-terminal asymmetric progenitor cell divisions, and require Mash1 for their development

Hendrik Wildner1, Thomas Müller1, Seo-Hee Cho2, Dominique Bröhl1, Constance L. Cepko2, Francois Guillemot3 and Carmen Birchmeier1,*

1 Max-Delbrück-Centrum for Molecular Medicine, Robert-Rössle-Strasse 10, 13125 Berlin-Buch, Germany.
2 Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
3 National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.

* Author for correspondence (e-mail: cbirch{at}mdc-berlin.de)

Accepted 3 March 2006

dILA and dILB neurons comprise the major neuronal subtypes generated in the dorsal spinal cord, and arise in a salt-and-pepper pattern from a broad progenitor domain that expresses the bHLH factor Mash1. In this domain, Mash1-positive and Mash1-negative cells intermingle. Using a Mash1GFP allele in mice, we show here that Mash1+ progenitors give rise to dILA and dILB neurons. Using retroviral tracing in the chick, we demonstrate that a single progenitor can give rise to a dILA and a dILB neuron, and that dILA neurons are the product of asymmetric progenitor cell divisions. In Mash1-null mutant mice, the development of dILA, but not of dILB neurons is impaired. We provide evidence that a dual function of Mash1 in neuronal differentiation and specification accounts for the observed changes in the mutant mice. Our data allow us to assign to Mash1 a function in asymmetric cell divisions, and indicate that the factor coordinates cell cycle exit and specification in the one daughter that gives rise to a dILA neuron.

Key words: Asymmetric cell division, Neuronal specification, Spinal cord, Mash1 (Ascl1), Mouse, Chick




This article has been cited by other articles:


Home page
 DevelopmentHome page
Y. Kimura, C. Satou, and S.-i. Higashijima
V2a and V2b neurons are generated by the final divisions of pair-producing progenitors in the zebrafish spinal cord
Development, September 15, 2008; 135(18): 3001 - 3005.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
H. Wildner, M. S. Gierl, M. Strehle, P. Pla, and C. Birchmeier
Insm1 (IA-1) is a crucial component of the transcriptional network that controls differentiation of the sympatho-adrenal lineage
Development, February 1, 2008; 135(3): 473 - 481.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
K. Hori, J. Cholewa-Waclaw, Y. Nakada, S. M. Glasgow, T. Masui, R. M. Henke, H. Wildner, B. Martarelli, T. M. Beres, J. A. Epstein, et al.
A nonclassical bHLH Rbpj transcription factor complex is required for specification of GABAergic neurons independent of Notch signaling
Genes & Dev., January 15, 2008; 22(2): 166 - 178.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Nakatani, Y. Minaki, M. Kumai, and Y. Ono
Helt determines GABAergic over glutamatergic neuronal fate by repressing Ngn genes in the developing mesencephalon
Development, August 1, 2007; 134(15): 2783 - 2793.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. C. Wilcock, J. R. Swedlow, and K. G. Storey
Mitotic spindle orientation distinguishes stem cell and terminal modes of neuron production in the early spinal cord
Development, May 15, 2007; 134(10): 1943 - 1954.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Shin, J. Poling, H.-C. Park, and B. Appel
Notch signaling regulates neural precursor allocation and binary neuronal fate decisions in zebrafish
Development, May 15, 2007; 134(10): 1911 - 1920.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Pillai, A. Mansouri, R. Behringer, H. Westphal, and M. Goulding
Lhx1 and Lhx5 maintain the inhibitory-neurotransmitter status of interneurons in the dorsal spinal cord
Development, January 15, 2007; 134(2): 357 - 366.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Battiste, A. W. Helms, E. J. Kim, T. K. Savage, D. C. Lagace, C. D. Mandyam, A. J. Eisch, G. Miyoshi, and J. E. Johnson
Ascl1 defines sequentially generated lineage-restricted neuronal and oligodendrocyte precursor cells in the spinal cord
Development, January 15, 2007; 134(2): 285 - 293.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
Q. Ma
Transcriptional regulation of neuronal phenotype in mammals
J. Physiol., September 1, 2006; 575(2): 379 - 387.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006