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First published online 16 August 2006
doi: 10.1242/dev.02510

Development 133, 3619-3628 (2006)
Published by The Company of Biologists 2006
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Fetal spleen stroma drives macrophage commitment

Julien Y. Bertrand1,2,*, Guillaume E. Desanti1,*, Richard Lo-Man3, Claude Leclerc3, Ana Cumano1 and Rachel Golub1,{dagger}

1 Unité du Développement des Lymphocytes, INSERM U668, Institut Pasteur, 25, Rue du Dr Roux, 75724 Paris cedex 15, France.
2 University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0380, USA.
3 Unité de Biologie des Regulations Immunitaires, INSERM E352, Institut Pasteur, 25, Rue du Dr Roux, 75724 Paris cedex 15, France.

{dagger} Author for correspondence (e-mail: rgolub{at}pasteur.fr)

Accepted 28 June 2006

The role of the fetal spleen in hematopoeisis remains largely unknown. In this particular environment, we show that hematopoietic stem cells do not proliferate, but that they lose multipotency and differentiate exclusively into mature macrophages. B lymphocytes in the spleen derive from committed B cell precursors that are likely to have immigrated from the fetal liver. We developed fetal spleen stromal cell lines that are unique in their capacity to expand myeloid precursors, resulting in large numbers of mature macrophages. These lines secrete high levels of anti-inflammatory molecules. By phenotype, fetal splenic macrophages are reminiscent of their adult counterparts found in the red pulp. We postulate that F4/80+ splenic macrophages participate in fetal erythropoiesis, as well as in the formation of the splenic architecture.

Key words: Hematopoiesis, Myeloid differentiation, Organogenesis




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