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First published online 16 April 2008
doi: 10.1242/dev.020958

Development 135, 1823-1832 (2008)
Published by The Company of Biologists 2008
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Developmental origin of smooth muscle cells in the descending aorta in mice

Per Wasteson1,2, Bengt R. Johansson3, Tomi Jukkola4, Silke Breuer1,*, Levent M. Akyürek1,2, Juha Partanen4 and Per Lindahl1,2,{dagger}

1 Wallenberg Laboratory, Sahlgrenska University Hospital, University of Gothenburg, Gothenburg, Sweden.
2 Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden.
3 Institute of Biomedicine, Electron Microscopy Unit, University of Gothenburg, Gothenburg, Sweden.
4 Institute of Biotechnology, University of Helsinki, Helsinki, Finland.

{dagger} Author for correspondence (e-mail: per.lindahl{at}wlab.gu.se)

Accepted 17 March 2008

Aortic smooth muscle cells (SMCs) have been proposed to derive from lateral plate mesoderm. It has further been suggested that induction of SMC differentiation is confined to the ventral side of the aorta, and that SMCs later migrate to the dorsal side. In this study, we investigate the origin of SMCs in the descending aorta using recombination-based lineage tracing in mice. Hoxb6-cre transgenic mice were crossed with Rosa 26 reporter mice to track cells of lateral plate mesoderm origin. The contribution of lateral plate mesoderm to SMCs in the descending aorta was determined at different stages of development. SMC differentiation was induced in lateral plate mesoderm-derived cells on the ventral side of the aorta at embryonic day (E) 9.0-9.5, as indicated by expression of the SMC-specific reporter gene SM22{alpha}-lacZ. There was, however, no migration of SMCs from the ventral to the dorsal side of the vessel. Moreover, the lateral plate mesoderm-derived cells in the ventral wall of the aorta were replaced by somite-derived cells at E10.5, as indicated by reporter gene expression in Meox1-cre/Rosa 26 double transgenic mice. Examination of reporter gene expression in adult aortas from Hoxb6-cre/Rosa 26 and Meox1-cre/Rosa 26 double transgenic mice suggested that all SMCs in the adult descending aorta derive from the somites, whereas no contribution was recorded from lateral plate mesoderm.

Key words: Vascular smooth muscle cell, Aorta, Lateral plate mesoderm, Paraxial mesoderm, Cell origin






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